A quantitative method to study co-adhesion of microorganisms in a parallel plate flow chamber: basic principles of the analysis

Vandermei, Hc; Meinders, Jm; Busscher, Hj; Bos, R.

doi:10.1016/0167-7012(94)90053-1

Cited by 59 publications

(49 citation statements)

References 24 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The clustering patterns observed were also observed in previous studies [73][74][75], but the positive and strong correlation between clustering and shear is novel and the level of turbulence in the flow developed to induce such high shear flows is unique in biofilm colonisation studies. The mechanisms suggested to be important for the clustering patterns are co-adhesion [76], which occurs on the substratum and describes the propensity for bacteria to attach in proximity to those that have already attached, and coaggregation [77], which is the coming-together of bacteria in clumps in the bulk water prior to adhesion onto the surface.…”

Section: Discussionmentioning

confidence: 99%

A Keystone Methylobacterium Strain in Biofilm Formation in Drinking Water

Tsagkari

Keating

Couto

et al. 2017

Water

View full text Add to dashboard Cite

Abstract:The structure of biofilms in drinking water systems is influenced by the interplay between biological and physical processes. Bacterial aggregates in bulk fluid are important in seeding biofilm formation on surfaces. In simple pure and co-cultures, certain bacteria, including Methylobacterium, are implicated in the formation of aggregates. However, it is unclear whether they help to form aggregates in complex mixed bacterial communities. Furthermore, different flow regimes could affect the formation and destination of aggregates. In this study, real drinking water mixed microbial communities were inoculated with the Methylobacterium strain DSM 18358. The propensity of Methylobacterium to promote aggregation was monitored under both stagnant and flow conditions. Under stagnant conditions, Methylobacterium enhanced bacterial aggregation even when it was inoculated in drinking water at 1% relative abundance. Laminar and turbulent flows were developed in a rotating annular reactor. Methylobacterium was found to promote a higher degree of aggregation in turbulent than laminar flow. Finally, fluorescence in situ hybridisation images revealed that Methylobacterium aggregates had distinct spatial structures under the different flow conditions. Overall, Methylobacterium was found to be a key strain in the formation of aggregates in bulk water and subsequently in the formation of biofilms on surfaces.

show abstract

Section: Discussionmentioning

confidence: 99%

A Keystone Methylobacterium Strain in Biofilm Formation in Drinking Water

Tsagkari

Keating

Couto

et al. 2017

Water

View full text Add to dashboard Cite

show abstract

“…This recognition between genetically distinct cells in suspension and resultant clumping is called coaggregation (72). Recognition between a suspended cell type and one already attached to a substratum is termed coadhesion (9). These interactions often appear to be mediated by complementary protein-adhesin and saccharide-receptor components on the two cell types (22,27,28,143,154).…”

Section: Introductionmentioning

confidence: 99%

Communication among Oral Bacteria

Kolenbrander

Andersen

Blehert

et al. 2002

Microbiol Mol Biol Rev

855

745

View full text Add to dashboard Cite

Human oral bacteria interact with their environment by attaching to surfaces and establishing mixed-species communities. As each bacterial cell attaches, it forms a new surface to which other cells can adhere. Adherence and community development are spatiotemporal; such order requires communication. The discovery of soluble signals, such as autoinducer-2, that may be exchanged within multispecies communities to convey information between organisms has emerged as a new research direction. Direct-contact signals, such as adhesins and receptors, that elicit changes in gene expression after cell-cell contact and biofilm growth are also an active research area. Considering that the majority of oral bacteria are organized in dense three-dimensional biofilms on teeth, confocal microscopy and fluorescently labeled probes provide valuable approaches for investigating the architecture of these organized communities in situ. Oral biofilms are readily accessible to microbiologists and are excellent model systems for studies of microbial communication. One attractive model system is a saliva-coated flowcell with oral bacterial biofilms growing on saliva as the sole nutrient source; an intergeneric mutualism is discussed. Several oral bacterial species are amenable to genetic manipulation for molecular characterization of communication both among bacteria and between bacteria and the host. A successful search for genes critical for mixed-species community organization will be accomplished only when it is conducted with mixed-species communities

show abstract

“…Two forms of physical interactions thought to play an important role in biofilm formation are coaggregation and coadhesion (2,5,6,9). Coaggregation is the cell-cell recognition between genetically distinct bacteria in a planktonic suspension (12), whereas coadhesion refers to the recognition between a planktonic cell and a surface-attached cell (3). Coaggregation and coadhesion are mediated by the same kinds of cell-cell interactions (5,35).…”

mentioning

confidence: 99%

Development of a Multispecies Oral Bacterial Community in a Saliva-Conditioned Flow Cell

Foster

Kolenbrander

2004

Appl Environ Microbiol

195

146

View full text Add to dashboard Cite

Microbial communities within the human oral cavity are dynamic associations of more than 500 bacterial species that form biofilms on the soft and hard tissues of the mouth. Understanding the development and spatial organization of oral biofilms has been facilitated by the use of in vitro models. We used a salivaconditioned flow cell, with saliva as the sole nutritional source, as a model to examine the development of multispecies biofilm communities from an inoculum containing the coaggregation partners Streptococcus gordonii, Actinomyces naeslundii, Veillonella atypica, and Fusobacterium nucleatum. Biofilms inoculated with individual species in a sequential order were compared with biofilms inoculated with coaggregates of the four species. Our results indicated that flow cells inoculated sequentially produced biofilms with larger biovolumes compared to those biofilms inoculated with coaggregates. Individual-species biovolumes within the four-species communities also differed between the two modes of inoculation. Fluorescence in situ hybridization with genusand species-specific probes revealed that the majority of cells in both sequentially and coaggregate-inoculated biofilms were S. gordonii, regardless of the inoculation order. However, the representation of A. naeslundii and V. atypica was significantly higher in biofilms inoculated with coaggregates compared to sequentially inoculated biofilms. Thus, these results indicate that the development of multispecies biofilm communities is influenced by coaggregations preformed in planktonic phase. Coaggregating bacteria such as certain streptococci are especially adapted to primary colonization of saliva-conditioned surfaces independent of the mode of inoculation and order of addition in the multispecies inoculum. Preformed coaggregations favor other bacterial strains and may facilitate symbiotic relationships.

show abstract

A quantitative method to study co-adhesion of microorganisms in a parallel plate flow chamber: basic principles of the analysis

Cited by 59 publications

References 24 publications

A Keystone Methylobacterium Strain in Biofilm Formation in Drinking Water

A Keystone Methylobacterium Strain in Biofilm Formation in Drinking Water

Communication among Oral Bacteria

Development of a Multispecies Oral Bacterial Community in a Saliva-Conditioned Flow Cell

Contact Info

Product

Resources

About