A quantitative model for lateral flow assays

Kler, Pablo A.

doi:10.1007/s10404-016-1771-9

Cited by 41 publications

(25 citation statements)

References 29 publications

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“…In other words, the binding of antibodies to the analyte-protein conjugate should be somewhat worse than with the native analyte. The influence of the characteristics of immunoreagents on the sensitivity of analysis is considered in detail in works devoted to the mathematical modeling of LFIA [25][26][27][28][29][30].…”

Section: Proper Receptor For Lfiamentioning

confidence: 99%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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This chapter considers factors influencing sensitivity of lateral flow immunoassay and modern developments that are focused on reaching lower detection limits. The existing variety of proposed approaches is classified in accordance with the "big five rules" for these assays, including proper sample, receptor, interaction, response, and output. The solutions for rapid extraction of target analytes and preventing negative influence of extractants are considered. Role to antibodies affinity and specificity is characterized. Potential of alternate bioreceptor molecules is discussed. Immunoreactants' compositions, concentrations, and locations on the test strip are characterized as factors determining assay parameters. The existing variety of labels is compared in terms of their optical and alternate registration. Tools to modulate a sequence of analytical reactions and to form aggregates of the detected labels are considered. The discussed approaches are illustrated through developments of test strips for detection of mycotoxins, veterinary drugs, and other analytes.The overall design of the immunochromatographic test strip is shown in Figure 1. It is a composite of several membranes of different structures and porosities, fixed on a support. The bundling of the test strip can vary, so it makes sense to consider its design based on what analytical tasks are being performed on its different sites.A. Typically, the lower portion of the test strip contains a sample pad. It ensures the absorption of sample components, in which the presence of the target analyte is checked.B. The following is a section with immunoreagents that are washed out during the analysis and move upward along with the components of the sample. As a rule, a conjugate pad forms this zone. It contains a conjugate of antibodies against the target analyte with a nanodispersed label-particles of colored latex, colloidal gold, and so on.The next two sections are located on the main working membrane of the test strip. C.First, there is a zone along which the movement of the absorbed components of the sample and the washed immunoreagents continues. During this movement, immune reactions occur, and specific intermolecular complexes are formed. D.Next, a mixture of reacted and unreacted molecules enters the binding area with immobilized immunoreagents. Depending on whether the target analyte was present in the sample and in what amount, binding of labeled immune complexes occurs in certain areas (in the traditional case, with the formation of narrow colored lines). Usually, additional reagents are located here to control the functionality of the test system. E. The upper part of the test strip with the final pad, usually structurally similar to the sample pad, ensures the further movement of the reaction mixture under the action of capillary forces and the washing of unreacted components from the underlying areas. These processes allow the label's binding to be evaluated correctly.Membrane components of the test strip are fixed on a plastic support and partia...

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Section: Proper Receptor For Lfiamentioning

confidence: 99%

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Zherdev¹,

Dzantiev²

2018

Rapid Test - Advances in Design, Format and Diagnostic Applications

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“…There are also some models for lateral flow assays that include not only the capillary flow in porous medium but also the advection of the analyte and the reactions taking place in the detection zone. 75 Earlier models include capillary conduction of liquids derived using Darcy's law and diffusion. 76 Darcy's law-based models are also used for the wicking in paper.…”

Section: Theoretical and Computational Approachesmentioning

confidence: 99%

A review on wax printed microfluidic paper-based devices for international health

2017

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Paper-based microfluidics has attracted attention for the last ten years due to its advantages such as low sample volume requirement, ease of use, portability, high sensitivity, and no necessity to well-equipped laboratory equipment and well-trained manpower. These characteristics have made paper platforms a promising alternative for a variety of applications such as clinical diagnosis and quantitative analysis of chemical and biological substances. Among the wide range of fabrication methods for microfluidic paper-based analytical devices (PADs), the wax printing method is suitable for high throughput production and requires only a commercial printer and a heating source to fabricate complex two or three-dimensional structures for multipurpose systems. PADs can be used by anyone for diagnosis and analysis; therefore, wax printed PADs are promising especially in resource limited environments where people cannot get sensitive and fast diagnosis of their serious health problems and where food, water, and related products are not able to be screened for toxic elements. This review paper is focused on the applications of paper-based microfluidic devices fabricated by the wax printing technique and used for international health. Besides presenting the current limitations and advantages, the future directions of this technology including the commercial aspects are discussed. As a conclusion, the wax printing technology continues to overcome the current limitations and to be one of the promising fabrication techniques. In the near future, with the increase of the current interest of the industrial companies on the paper-based technology, the wax-printed paper-based platforms are expected to take place especially in the healthcare industry.

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“…Desde luego existen modelos numéricos rigurosos para abordar los problemas de transporte de especies con reacción química en geometrías arbitrarias (58-60), pero su imple-mentación requiere cálculo computacional y usuarios expertos. En este sentido, hemos trabajado en la elaboración de un modelo matemático relativamente simple, de solución analítica, el cual es capaz de describir cuantitativamente los procesos de transporte y reacción química en dispositivos de análisis basados en flujo lateral (61).…”

Section: Trasporte De Fluidosunclassified

“…Allí el analito ingresa a la concentración C 0 y reacciona en sitios de captura fijos en la membrana, donde tiene lugar una reacción de tipo antígeno-anticuerpo con constante de equilibrio K. La formulación incluye el flujo capilar de la muestra, cuya velocidad en la línea de captura es u L = c/x L (c ∼ de 1 mm 2 /s para agua en nitrocelulosa). Las predicciones se validaron con simulaciones numéricas del problema completo (61). A manera de ilustración, la Fig.…”

Section: Trasporte De Fluidosunclassified

Dispositivos de microfluídica basados en papel: orígenes, presente y perspectivas

Berli

2017

FABICIB

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Los dispositivos de microfluídica denominados laboratorios en chips se han consolidado como la nueva tecnología para ensayos de laboratorio y sus aplicaciones continúan expandiéndose. En este ámbito de permanente innovación, el papel ha emergido recientemente como un sustrato de gran interés, particularmente para fabricar dispositivos portables, autónomos y de bajo costo, con aplicaciones en diagnóstico médico, cuidado personal, control de calidad de alimentos y monitoreo ambiental. Los microdispositivos se acoplan además a teléfonos móviles para realizar el análisis y la transmisión de datos, lo cual potencia enormemente su desempeño. En este trabajo se realiza una breve revisión el tema, destacando algunos avances recientes en la investigación, y se discuten las perspectivas para desarrollar la tecnología en nuestra región.

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A quantitative model for lateral flow assays

Cited by 41 publications

References 29 publications

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

Ways to Reach Lower Detection Limits of Lateral Flow Immunoassays

A review on wax printed microfluidic paper-based devices for international health

Dispositivos de microfluídica basados en papel: orígenes, presente y perspectivas

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