A Quantitative One-Stage Method for the Assay of Prothrombin

Owren, P. A.

doi:10.3109/00365514909065629

Cited by 166 publications

(34 citation statements)

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“…Owren's method (15) for the assay of prothrombin was used. Rabbit brain thromboplastin (Factor III) was prepared from the gray substance after an exhaustive carotid-jugular perfusion of the cranial vessels of freshly sacrificed animals.…”

Section: Methodsmentioning

confidence: 99%

In vivo effects of Agkistrodon rhodostoma venom: studies with fibrinogen-131I.

Regoeczi¹,

Gergely²,

McFarlane³

1966

J. Clin. Invest.

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Section: Methodsmentioning

confidence: 99%

In vivo effects of Agkistrodon rhodostoma venom: studies with fibrinogen-131I.

Regoeczi¹,

Gergely²,

McFarlane³

1966

J. Clin. Invest.

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“…Tissue factor wvas a saline extract of human brain tissue (7) centrifuged twice at 12,000 g to remove large particulate material. It clotted recalcified normal plasma in 17 s. A reference standard plasma assumed to contain 1 U/ml activity of all clotting factors was prepared by pooling plasma from 12 healthy males and was stored at -20°C.…”

Section: Reagentsmentioning

confidence: 99%

Activation of Human Factor VII in Plasma and in Purified Systems

Seligsohn¹,

Østerud²,

Brown³

et al. 1979

J. Clin. Invest.

158

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A B S T R A C T Factor VII can be activated, to a molecule giving shorter clotting times with tissue factor, by incubating plasma with kaolin or by clotting plasma. The mechanisms of activation differ. With kaolin, activated Factor XII (XIIa) was the apparent principal activator. Thus, Factor VII was not activated in Factor XII-deficient plasma, was partially activated in prekallikrein and high-molecular weight kininogen (HMW kininogen)-deficient plasmas, but was activated in other deficient plasmas. After clotting, activated Factor IX (IXa) was the apparent principal activator. Thus, Factor VII was not activated in Factor XII-, HMW kininogen-, XI-, and IX-deficient plasmas, but was activated in Factor VIII-, X-, and V-deficient plasmas. In further studies, purified small-fragment Factor XIIa (,8-XIIa), kallikrein, and Factor IXa were added to partially purified Factor VII and to plasma.

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“…Only 9 of 35 patients with severe and moderate haemophilia B investigated by Orstavik (14) had f.IX: Ag below the concentration demonstrable by the method (2 U/ dl). In the other studies on record (1,7,9,16,21) it was not possible to detect f.IX:Ag levels below 7-10 U/dl).…”

Section: Ljung and Holmbergmentioning

confidence: 99%

“…after it had become possible to determine factor IX antigen (f.IX: Prothrombin and Proconvertin was measured to lhe Ag) using different assays (1,7,9,11,14,15,16,17,19,20,21, method of o w e n (IS), which measures the sum of the vitamin K-22). cross reacting material negative (CRM-) variants have no dependent coagulation factors, i.e., prothrombin, factor VII and demonstrable f.IX:Ag; CRM+ variants have normal amounts, and factor X.…”

Section: Patientsmentioning

confidence: 99%