A quantitative real-time RT-PCR assay for salmon IGF-I mRNA, and its application in the study of GH regulation of IGF-I gene expression in primary culture of salmon hepatocytes

Pierce, Andrew L.; Dickey, J. F.; Larsen, Donald A.; Fukada, Haruhisa; Swanson, Penny; Dickhoff, Walton W.

doi:10.1016/j.ygcen.2003.10.010

Cited by 94 publications

(65 citation statements)

References 39 publications

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“…The standard stimulatory concentration of 5 10 9 M sGH was based on previous sGH concentration-response studies (Pierce et al 2004a). The survey of hormonal effects (Figs 1 and 5) was performed on cells cultured separately from three female fish, 149·7 4·1 g (mean S.E.M.)…”

Section: Methodsmentioning

confidence: 99%

“…Hepatocytes were isolated and cultured by the procedure of Mommsen and co-workers, as previously described (Pierce et al 2004a). Fish were fasted for 1 day before isolation of hepatocytes.…”

Section: Hepatocyte Culture and Rna Isolationmentioning

confidence: 99%

“…Cultures were stopped 18 h later by adding Tri-Reagent (MRC, Cincinnati, OH, USA) to wells. The 18-h time point was selected on the basis of a previous time-course experiment (Pierce et al 2004a). RNA was harvested according to the MRC protocol, with bromochloropropane as the phase separation reagent and two 70% ethanol washes.…”

Section: Hepatocyte Culture and Rna Isolationmentioning

confidence: 99%

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Metabolic hormones modulate the effect of growth hormone (GH) on insulin-like growth factor-I (IGF-I) mRNA level in primary culture of salmon hepatocytes

Pierce

Fukada²,

Dickhoff³

2005

Journal of Endocrinology

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Liver production of insulin-like growth factor-I (IGF-I) is a major point of control in the growth hormone (GH)/IGF axis, the endocrine system regulating body growth in fishes and other vertebrates. Pituitary GH stimulates hepatocyte production of IGF-I; however, in catabolic states, hepatocyte GH resistance results in decreases in liver IGF-I production. To investigate endocrine mechanisms leading to the development of hepatocyte GH resistance, we examined the regulation of IGF-I mRNA level by GH and metabolic hormones in primary culture of salmon hepatocytes. Cells were cultured in RPMI medium, and exposed to insulin (Ins, 10 6

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Section: Methodsmentioning

confidence: 99%

Section: Hepatocyte Culture and Rna Isolationmentioning

confidence: 99%

Section: Hepatocyte Culture and Rna Isolationmentioning

confidence: 99%

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Metabolic hormones modulate the effect of growth hormone (GH) on insulin-like growth factor-I (IGF-I) mRNA level in primary culture of salmon hepatocytes

Pierce

Fukada²,

Dickhoff³

2005

Journal of Endocrinology

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“…This standard was assigned an arbitrary value and then diluted serially in tenfold increments to generate the standard curve used to extrapolate gene copy numbers in the samples. The expression levels of each immune-response gene were normalized against the expression level of a housekeeping gene, acidic ribosomal phosphoprotein P0 (ARP) (Pierce et al, 2004;Purcell et al, 2004). Analysis and calculation of fold change relative to mock-infected control groups has been previously described (Purcell et al, 2004).…”

Section: Viral Load Data Were Calculated As Gene Copies (Mg Rna)mentioning

confidence: 99%

Differential virulence mechanisms of infectious hematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss) include host entry and virus replication kinetics

Peñaranda

Purcell

Kurath

2009

Journal of General Virology

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Host specificity is a phenomenon exhibited by all viruses. For the fish rhabdovirus infectious hematopoietic necrosis virus (IHNV), differential specificity of virus strains from the U and M genogroups has been established both in the field and in experimental challenges. In rainbow trout (Oncorhynchus mykiss), M IHNV strains are consistently more prevalent and more virulent than U IHNV. The basis of the differential ability of these two IHNV genogroups to cause disease in rainbow trout was investigated in live infection challenges with representative U and M IHNV strains. When IHNV was delivered by intraperitoneal injection, the mortality caused by U IHNV increased, indicating that the low virulence of U IHNV is partly due to inefficiency in entering the trout host. Analyses of in vivo replication showed that U IHNV consistently had lower prevalence and lower viral load than M IHNV during the course of infection. In analyses of the host immune response, M IHNV-infected fish consistently had higher and longer expression of innate immunerelated genes such as Mx-1. This suggests that the higher virulence of M IHNV is not due to suppression of the immune response in rainbow trout. Taken together, the results support a kinetics hypothesis wherein faster replication enables M IHNV to rapidly achieve a threshold level of virus necessary to override the strong host innate immune response.

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“…The number of insulin and IGF receptors in fish is regulated by the nutritional status so that it can be altered according to the physiological need (Planas et al, 2000). Reports are available on the levels of serum IGF-1 in different fish species such as chinook salmon Oncorhynchus tshawytscha (Beckman et al, 2004a;b), coho salmon O. kisutch (Pierce et al, 2004;Beckman et al, 2004a;b), Atlantic salmon Salmo salar (Dyer et al, 2004;Shimizu et al, 2006), tilapia Oreochromis mossambicus (Uchida et al, 2003), gilthead seabream Sparus aurata (Pé rez-Sá nchez et al, 1995;Mingarro et al, 2002;Gómez-Requeni et al, 2004;2005), channel catfish Ictalurus punctatus (Silverstein et al, 2000;Li et al, 2004) and Atlantic cod Gadus morhua (Davie et al, 2007). Li et al (2014) used transgenic crucian carp, Carassius auratus as the first teleost model to study the IGF-1 over expression in-vivo.…”

Section: Introductionmentioning

confidence: 99%

Effect of Culture Conditions on the Levels of Serum Insulin-like Growth Factor-1 in Indian Major Carps

Abraham

Hoque²,

Das³

et al. 2017

ija

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The insulin-like growth factor-1 (IGF-1) plays an important role in the regulation of development and growth of fish.Aquaculture researchers consider it as a potential growth rate indicator. Reports on the IGF-1 of carps are limited. This study was undertaken to determine the serum IGF-1 levels of Indian major carps (IMCs) cultured in the normal pond, sewage-fed pond and captive conditions. Labeo rohita of the normal pond recorded the highest serum IGF-1 level (2.10±0.14 ng/ml), followed by Catla catla (1.99±0.17 ng/ml) and Cirrhinus mrigala (1.82±0.12 ng/ml). Captive held C. catla recorded the highest serum IGF-1 (2.10±0.19 ng/ml) compared to L. rohita (2.04±0.08 ng/ml) and C. mrigala (1.90±0.09 ng/ml). In the sewage-fed pond, C. catla, L. rohita and C. mrigala recorded the serum IGF-1 levels of 2.38±0.36 ng/ml, 2.06 ±0.03 ng/ml and 1.07±0.06 ng/ml, respectively. Significant differences existed among the serum IGF-1 levels of C. mrigala reared in the normal and sewage-fed pond as well as the captive and sewage-fed pond (P < 0.05). It appears from the results that C. catla and L. rohita are the ideal cultivable species under the sewage-fed aquaculture. The serum IGF-1 level may be an effective indicator of the differences in growth of carps.

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A quantitative real-time RT-PCR assay for salmon IGF-I mRNA, and its application in the study of GH regulation of IGF-I gene expression in primary culture of salmon hepatocytes

Cited by 94 publications

References 39 publications

Metabolic hormones modulate the effect of growth hormone (GH) on insulin-like growth factor-I (IGF-I) mRNA level in primary culture of salmon hepatocytes

Metabolic hormones modulate the effect of growth hormone (GH) on insulin-like growth factor-I (IGF-I) mRNA level in primary culture of salmon hepatocytes

Differential virulence mechanisms of infectious hematopoietic necrosis virus in rainbow trout (Oncorhynchus mykiss) include host entry and virus replication kinetics

Effect of Culture Conditions on the Levels of Serum Insulin-like Growth Factor-1 in Indian Major Carps

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