A quantitative, semi-automated and computer-assisted test for lupus anticoagulant

Schjetlein, Rune; Sletnes, Kari E.; Wisløff, Finn

doi:10.1016/0049-3848(93)90049-t

Cited by 37 publications

(35 citation statements)

References 12 publications

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“…[19][20][21] For each of the 3 tests, in-house reagents with dilutions of crude cephalin from porcine brain (a generous gift from Axis-Shield PoC AS, Oslo, Norway) were used. In the APTT-based LR test, crude cephalin diluted 1:50 and 1:800 in Owren buffer was mixed 1:1 with ellagic acid as activator (final concentration 6 mg/L).…”

Section: Measurement Of Lasmentioning

confidence: 99%

Clinical significance of different antiphospholipid antibodies in the WAPS (warfarin in the antiphospholipid syndrome) study

Galli

Borrelli

Jacobsen³

et al. 2007

Blood

161

143

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To assess the clinical significance of lupus anticoagulants (LAs) and antiphospholipid antibodies (aPLs) toward thrombosis and abortions, we measured them in 112 patients whose samples were available at enrollment in the warfarin in the antiphospholipid syndrome (WAPS) study. Enzyme-linked immunosorbent assay (ELISA) and coagulation test values in the highest and lowest tertiles were compared. When considered separately, IgG antibodies to ␤2-glycoprotein I (a␤2GPI) and prothrombin (aPT) were associated with anamnestic arterial and venous thrombosis, respectively, and those to annexin AV (aAnAV) with abortions. IgM antibodies to protein S and the lupus ratio of the dilute prothrombin time were associated with prospective thrombosis. No other association for IgM antibodies was seen. LA-positive patients who carried a␤2GPI antibodies were at risk of anamnestic arterial and total thrombosis and aPT antibodies to that of anamnestic venous and total thrombosis. LA-positive patients who carried IgG a␤2GPI and aAnAV antibodies were at risk for both anamnestic abortion and prospective thrombosis. Overall, these data support the inclusion of a␤2GPI antibodies in and suggest the removal of anticardiolipin antibodies from the laboratory criteria of the antiphospholipid syndrome. They also suggest that the measurement of aPT and aAnAV antibodies is useful in some selected situations and that there is little role for IgM antibody detection. (Blood.

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Section: Measurement Of Lasmentioning

confidence: 99%

Clinical significance of different antiphospholipid antibodies in the WAPS (warfarin in the antiphospholipid syndrome) study

Galli

Borrelli

Jacobsen³

et al. 2007

Blood

161

143

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“…A number of integrated systems are now available for incorporating a screening assay, mixing study and con®rmatory procedure into a single battery [9,18,19].…”

Section: Discussionmentioning

confidence: 99%

Recurrent antiphospholipid-related deep vein thrombosis as presenting manifestation of systemic lupus erythematosus

Gattorno¹,

Molinari

Buoncompagni³

et al. 2000

European Journal of Pediatrics

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“…Therefore assays with high interlaboratory agreement are urgently needed. The APTT-based LR test has integrated screening, mixing and confirmatory procedures into one assay (8) and attains high interlaboratory agreement regarding the presence or absence of LA as well as the semiquantification of this inhibitor (9). Our assay system was based on this previously described LR test and can be performed with commercially available reagents.…”

Section: Specificity and Sensitivitymentioning

confidence: 99%

“…The APTT-based lupus ratio (LR) test has integrated screening, mixing, and confirmatory procedures into one assay (8) and attains high interlaboratory agreement regarding the presence or absence of LA as well as the semi-quantification of this inhibitor (9). Based on the test-principle of the LR test, we developed an automated, sensitive aPTT-based assay (MIXCON-LA) for detection of lupus anticoagulant with good dicrimination from oral anticoagulants and heparin.…”

mentioning

confidence: 99%

MIXCON-LA: A Precise, Sensitive and Specific aPTT-Based Assay for Detection of Lupus Anticoagulant

Lindhoff-Last

Humpich²,

Schmitt³

et al. 2002

Clin Appl Thromb Hemost

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Lupus anticoagulants (LA) are associated with an increased risk of thrombosis and laboratory detection is of major importance. Multiple tests are available for screening and confirmation, but they differ in sensitivity and specificity, frequently lacking the ability to discriminate between the presence of LA, heparin, and oral anticoagulants. Based on the test-principle of the Lupus Ratio-test, an automated, sensitive APTT-based assay, using mixtures of a lupussensitive and a lupusinsensitive APTT-reagent with normal plasma for detection of lupus anticoagulants was developed. Ninety-nine healthy volunteers, ten patients treated with unfractionated heparin intravenously, 19 patients taking stable oral anticoagulation, five patients with hemophilia A, and 15 patients with antiphospholipid-antibody-syndrome (APS) were investigated. In all patients, two APTTs were performed, one with each reagent, on 1:1 mixtures of test plasma and normal plasma (MIXCON-LA assay). The ratio between the two clotting times was divided by the corresponding ratio for the normal plasma. This final lupus ratio (LR) was used for evaluation. The within-series imprecision and the between-series imprecision were excellent with coefficients of variation between 1.5% and 1.9%. The mean +/- 2 SD of the LR of the 99 healthy volunteers was used as reference range (LR: 0.95-1.07). All patients treated either with heparin or oral anticoagulants remained negative in the MLXCON-LA assay (specificity, 100%), while one of five patients with hemophilia A, in whom a factor VIII-inhibitor developed, showed a false-positive result. In 13 of 15 patients with APS, an increased ratio was observed (sensitivity, 87%). This assay system allows precise, specific, and sensitive detection of lupus anticoagulants.

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A quantitative, semi-automated and computer-assisted test for lupus anticoagulant

Cited by 37 publications

References 12 publications

Clinical significance of different antiphospholipid antibodies in the WAPS (warfarin in the antiphospholipid syndrome) study

Clinical significance of different antiphospholipid antibodies in the WAPS (warfarin in the antiphospholipid syndrome) study

Recurrent antiphospholipid-related deep vein thrombosis as presenting manifestation of systemic lupus erythematosus

MIXCON-LA: A Precise, Sensitive and Specific aPTT-Based Assay for Detection of Lupus Anticoagulant

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