Background:Based on previous in vitro studies, it has been commonly believed that during anterior cruciate ligament (ACL) reconstruction with hamstring tendon, the grafted tendon is shifted anteriorly in the tunnel permanently after the graft is anchored to the tunnel wall. However, this has not been proven by in vivo studies.Hypothesis:At 1 year after anatomic double-bundle ACL reconstruction, the grafted tendons may not be shifted anteriorly in the femoral tunnel but anchored to the bony wall at the center of the tunnel.Study Design:Case series; Level of evidence, 4.Methods:Participants consisted of 40 patients who underwent anatomic double-bundle ACL reconstruction. The grafted tendons located in the femoral tunnel were examined 1 year after surgery using 2 different magnetic resonance imaging (MRI) protocols. In the first substudy, with 20 patients, the grafted tendon location was evaluated on an inclined sagittal multiplanar reconstruction (MPR) image taken using a standard T2-weighted protocol. In the second substudy with the remaining 20 patients, tendon location was evaluated on a pure axial MPR image taken using a VISTA (volume isotropic turbo spin echo acquisition) protocol.Results:On the inclined sagittal T2-weighted images of the anteromedial (AM) graft, the anterior width of the newly formed fibrous tissue, which surrounded the tendon graft, was significantly greater than the posterior width (P = .001). The center of the grafted tendon was slightly (mean, 2.5% of the tunnel diameter) but significantly (P = .0310) shifted posteriorly from the tunnel center. On the axial T2-VISTA images, the center of the AM graft was slightly but significantly shifted posteriorly (3.9%; P = .022) and medially (5.5%; P = .002) from the tunnel center. The center of the posterolateral (PL) graft was not significantly shifted to any direction from the center of the tunnel.Conclusion:The grafted tendons were not shifted anteriorly in the femoral tunnel 1 year after anatomic double-bundle ACL reconstruction. The PL graft was located approximately at the center of the tunnel outlet, while the AM graft was slightly but significantly shifted posteriorly and proximally.