A quick and inexpensive method to determine 2,4-dichlorophenoxyacetic acid residues in water samples by HPLC

Orooji, Naghmeh; Takdastan, Afshin; Yengejeh, Reza Jalilzadeh; Jorfi, Sahand; Davami, Amir Hossein

doi:10.5004/dwt.2021.26905

Cited by 9 publications

(4 citation statements)

References 30 publications

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“…One of the most important parameters that can affect the extraction is the pH of the sample. 40 Hence, the pH variations between the different media are expected to influence the final extraction yield. Overall, the extraction efficiencies were found to be in the same order of magnitude, irrespective of the matrix, and indicate proximity to the values estimated from the calibration curve.…”

Section: Resultsmentioning

confidence: 99%

“…BSM and FM22 media are observed to have a lower EE, which indicates that the extraction is impacted by the presence of various salts present in the media. One of the most important parameters that can affect the extraction is the pH of the sample . Hence, the pH variations between the different media are expected to influence the final extraction yield.…”

Section: Resultsmentioning

confidence: 99%

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Processing Method for the Quantification of Methanol and Ethanol from Bioreactor Samples Using Gas Chromatography–Flame Ionization Detection

2022

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Methanol, a simple polar solvent, has been widely identified as an attractive carbon source to produce chemicals and fuels in bioprocesses. Specifically, to achieve recombinant protein production from methylotrophic yeasts, such as Pichia pastoris , this organic solvent can be used as a sole carbon source for growth and maintenance as well as an inducer for protein expression. However, if methanol feeding is not controlled well in such a fermentation process, accumulation of the solvent in the growth media will have a detrimental effect on the cells. Hence, monitoring the levels of methanol in these fermentation processes is a crucial step to ensure a healthy culture and maximum protein production. There are various techniques elaborated in the literature for monitoring methanol in cell cultures, but often, they appear to be expensive methods that are less affordable for many laboratories. This is because, in addition to the sophisticated equipment that is required for the analysis, the complexity of the samples retrieved from the bioprocesses necessitates laborious processing steps often involving expensive tools. In this study, a fast, simple, and sensitive method is developed to process biological samples by using the salting-out-assisted liquid–liquid extraction technique to quantify the concentration of methanol and ethanol using gas chromatography. On comparing the combinations of widely available salts and solvents, it was noticed that salting out using potassium carbonate followed by the liquid–liquid extraction of the analyte using ethyl acetate showed the best recovery. Followed by this, a validation test for the developed method was performed, which resulted in good peak resolution, linearity, and limit of detection for the quantitation of methanol and ethanol. By further assessing the tested combination, it was confirmed that its application could be extended to other matrices. Such an approach facilitates the possibility to monitor and control the methanol levels in fermentation and aids in bioprocess optimization.

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Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

Processing Method for the Quantification of Methanol and Ethanol from Bioreactor Samples Using Gas Chromatography–Flame Ionization Detection

2022

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“…A direct comparison of the detecting platform developed herein with those reported in the literature is provided in Table S1. † As we can see, the recovery of the PPDA@Au SERS platform was better than that of the previously reported methods, including by HPLC-MS. [42][43][44][45]…”

Section: Analytical Methods Papermentioning

confidence: 99%

High-efficiency SERS platform based on 3D porous PPDA@Au NPs as a substrate for the detection of pesticides on vegetables

Zeng,

Yang,

Cao

et al. 2023

Anal. Methods

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“…To date, various methods for detecting 2,4-D have been established, such as high performance liquid chromatography (HPLC) [3,4], liquid chromatography mass spectrometry (LC-MS) [5,6], gas chromatography (GC) [7], and GC-MS [8,9]. However, there are some drawbacks to these techniques.…”

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confidence: 99%

A magnetic molecular imprinting‐based fluorescence probe for sensitive and selective detection of 2,4‐D herbicide

Chu,

Yu,

Meng

et al. 2023

Luminescence

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A new magnetic molecular imprinting‐based turn‐on fluorescence probe (Fe3O4NPs@SiO2@NBD@MIPs) has been synthesized via a facile sol–gel polymerization for the detection of 2,4‐dichlorophenoxyacetic acid (2,4‐D). Based on the photoinduced electron transfer (PET) of nitrobenzoxadiazole (NBD), 2,4‐D can be recognized by enhancement of NBD fluorescence. With the presence of Fe3O4 in the core of the probe, this sensor can also be reused many times using magnetic aggregation methods. After the addition of various concentrations of 2,4‐D, the fluorescence peak at 530 nm (excitation of 468 nm) increased linearly ranging from 0.1 to 3 μM with a detection limit of 0.023 μM. This sensing system is believed to be available for detecting 2,4‐D in real samples, with high recovery rates ranging from 94% to 108% for three spike levels of 2,4‐D with precisions below 5%.

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A quick and inexpensive method to determine 2,4-dichlorophenoxyacetic acid residues in water samples by HPLC

Cited by 9 publications

References 30 publications

Processing Method for the Quantification of Methanol and Ethanol from Bioreactor Samples Using Gas Chromatography–Flame Ionization Detection

Processing Method for the Quantification of Methanol and Ethanol from Bioreactor Samples Using Gas Chromatography–Flame Ionization Detection

High-efficiency SERS platform based on 3D porous PPDA@Au NPs as a substrate for the detection of pesticides on vegetables

A magnetic molecular imprinting‐based fluorescence probe for sensitive and selective detection of 2,4‐D herbicide

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