A quick and sensitive diagnostic tool for detection of Maize streak virus

Tembo, Mathias; Adediji, Adedapo Olutola; Bouvaine, Sophie; Chikoti, Patrick Chiza; Seal, Susan; Silva, Gonçalo

doi:10.1038/s41598-020-76612-2

Cited by 13 publications

(3 citation statements)

References 39 publications

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“…Previous studies have shown that RT-LAMP is at least 100 times more sensitive than RT-PCR [ 23 , 46 , 47 ]. Nkere et al [ 23 ] reported a sensitivity limit of 1000 fg/µL–100 fg/µL and 0.1 ng/µL–0.01 ng/µL for YMV LAMP primers, and YMV-F3x and YMV-B3x PCR primers, respectively.…”

Section: Discussionmentioning

confidence: 99%

Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the Rapid and Sensitive Detection of Yam mosaic virus

Festus

Seal

Prempeh

et al. 2023

Viruses

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Yam (Dioscorea spp.) productivity is constrained significantly by the lack of a formal seed system. Vegetative propagation, through tuber setts as ‘seed’ yams, encourages the recycling of virus-infected planting materials, contributing to high virus incidence and yield losses. Efforts are ongoing to increase the production of high-quality seed yams in a formal seed system to reduce virus-induced yield losses and enhance the crop’s productivity and food security. Specific and sensitive diagnostic tests are imperative to prevent the multiplication of virus-infected materials contributing to a sustainable seed yam certification system. During routine indexing of yam accessions, discrepancies were observed between the results obtained from the reverse transcription loop-mediated isothermal amplification (RT-LAMP) test and those from reverse transcription polymerase chain reaction (RT-PCR); RT-LAMP failed to detect Yam mosaic virus (YMV) in some samples that tested positive by RT-PCR. This prompted the design of a new set of LAMP primers, YMV1-OPT primers. These primers detected as little as 0.1 fg/µL of purified RNA obtained from a YMV-infected plant, a sensitivity equivalent to that obtained with RT-PCR. RT-LAMP using YMV1-OPT primers is recommended for all future virus-indexing of seed yams for YMV, offering a rapid, sensitive, and cost-effective approach.

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Section: Discussionmentioning

confidence: 99%

Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the Rapid and Sensitive Detection of Yam mosaic virus

Festus

Seal

Prempeh

et al. 2023

Viruses

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“…In future, the use of quick and sensitive diagnostic tool for detection of MSV directly in rice field, like loop-mediated isothermal amplification (LAMP) assays (Tembo et al 2020), will help to unraveled the association between MSV infection and symptoms in rice, and will contribute to better know the MSV epidemiology in rice fields from Africa.…”

Section: Discussionmentioning

confidence: 99%

Old foe, new host: epidemiology, genetic diversity and pathogenic characterization of maize streak virus in rice fields from Burkina Faso

Fouad,

Barro,

Bangratz

et al. 2023

Preprint

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Rice is of critical significance regarding food security worldwide including in Africa. Only two viruses impacting rice production in Africa have been deeply investigated for decades: the rice yellow mottle virus (Solemoviridae) and the rice stripe necrosis virus (Benyviridae). Using viral metagenomics, we aimed at exploring the diversity of viruses circulating in Burkina Faso rice fields. We performed an epidemiological survey in this country between 2016 and 2019 involving 57 small farmer’s rice fields under two production systems (rainfed lowlands and irrigated areas). More than 2700 rice samples were collected withouta priori(not based on symptom observation) following a regular scheme. In addition, wild and cultivated (maize and sugarcane)Poaceaegrowing nearby rice fields were also collected. Unexpectedly, metagenomics detected maize streak virus (MSV,Geminiviridae) in analyzed rice samples. Further molecular analyses using RCA-PCR showed that MSV is widely distributed and highly prevalent in both rainfed lowlands and irrigated rice areas. MSV-A and MSV-G strains were identified. MSV-G, exclusively identified so far in wild grasses, was the most prevalent strain while MSV-A, known to cause severe symptoms in maize, was sporadically identified. No genetic differentiation was detected between MSV isolates either infecting wild or cultivated plant species. Using infectious clones in experimental conditions, we confirmed the pathogenicity of both MSV strains on rice. Thus, in addition to contribute to the epidemiological surveillance of rice production in Africa, our results illuminate new epidemiological and pathogenic aspects of one of the most studied plant viruses with significant economic consequences in Africa.FundingFrench National Research Agency «Investissements d’avenir» program (ANR-10-LABX-001-01), Agropolis Fondation (ANR-16-IDEX-006), French National Research Agency “young researchers” program (ANR-20-CE35-0008-01), CGIAR Research Program on Rice Agri-food Systems (RICE), Cooperation and cultural action department of the French Embassy (SCAC) in Burkina Faso.

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“…With the help of two different loop primers, more DNA products could be synthesized, and the resulting products can be visualized by agarose gel electrophoresis and/or a naked-eye system. LAMP has been used to detect TYLCV, maize streak virus, and several other begomoviruses, such as squash leaf curl virus, tomato leaf curl Bangalore virus, and tomato leaf curl New Delhi virus (Fukuta et al 2003;Kuan et al 2010;Tembo et al 2020). The major limitation of the LAMP method is primer design.…”

Section: Nucleic Acid-based Detection Of Tylcvmentioning

confidence: 99%

Occurrence, distribution, and management of tomato yellow leaf curl virus in China

Qiao

Yang

et al. 2022

Phytopathol Res

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Tomato yellow leaf curl virus (TYLCV), belonging to the genus Begomovirus in the family Geminiviridae, is the most damaging virus for global tomato production. This virus has become one of the most studied plant viral pathogens because of its huge economic importance. Since it was firstly identified in Shanghai, China, in 2006, it has been spread to most parts of the country. The widespread occurrence, rapid spread to new regions, and enormous damage to tomato production, makes it an important agricultural pathogen in China. TYLCV has been characterized extensively at the molecular level. This review is focused on the occurrence and distribution of all TYLCV isolates in China, providing valuable information for further epidemiological studies. In addition, management strategies for TYLCV are also proposed, with the ultimate goal to prevent and control the further occurrence of this viral disease.

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A quick and sensitive diagnostic tool for detection of Maize streak virus

Cited by 13 publications

References 39 publications

Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the Rapid and Sensitive Detection of Yam mosaic virus

Improved Reverse Transcription Loop-Mediated Isothermal Amplification (RT-LAMP) for the Rapid and Sensitive Detection of Yam mosaic virus

Old foe, new host: epidemiology, genetic diversity and pathogenic characterization of maize streak virus in rice fields from Burkina Faso

Occurrence, distribution, and management of tomato yellow leaf curl virus in China

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