A quorum-sensing inhibitor blocks
            <i>Pseudomonas aeruginosa</i>
            virulence and biofilm formation

O’Loughlin, Colleen T.; Miller, Laura C.; Siryaporn, Albert; Drescher, Knut; Semmelhack, M. F.; Bassler, Bonnie L.

doi:10.1073/pnas.1316981110

Cited by 659 publications

(598 citation statements)

References 36 publications

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“…Thus, alternatives to conventional treatments for P. aeruginosa infection are urgently needed. QS inhibitors reduce P. aeruginosa virulence (44). Similarly, phage therapy targeting clinical P. aeruginosa isolates enhances survival of mice (45).…”

Section: Significancementioning

confidence: 99%

Quorum sensing controls the Pseudomonas aeruginosa CRISPR-Cas adaptive immune system

Høyland‐Kroghsbo

Paczkowski

Mukherjee

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

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264

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CRISPR-Cas are prokaryotic adaptive immune systems that provide protection against bacteriophage (phage) and other parasites. Little is known about how CRISPR-Cas systems are regulated, preventing prediction of phage dynamics in nature and manipulation of phage resistance in clinical settings. Here, we show that the bacterium Pseudomonas aeruginosa PA14 uses the cell-cell communication process, called quorum sensing, to activate cas gene expression, to increase CRISPR-Cas targeting of foreign DNA, and to promote CRISPR adaptation, all at high cell density. This regulatory mechanism ensures maximum CRISPR-Cas function when bacterial populations are at highest risk for phage infection. We demonstrate that CRISPR-Cas activity and acquisition of resistance can be modulated by administration of proand antiquorum-sensing compounds. We propose that quorum-sensing inhibitors could be used to suppress the CRISPR-Cas adaptive immune system to enhance medical applications, including phage therapies.quorum sensing | CRISPR | immunity | phage | phage defense

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Section: Significancementioning

confidence: 99%

Quorum sensing controls the Pseudomonas aeruginosa CRISPR-Cas adaptive immune system

Høyland‐Kroghsbo

Paczkowski

Mukherjee

et al. 2016

Proc. Natl. Acad. Sci. U.S.A.

Self Cite

264

223

View full text Add to dashboard Cite

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“…For example, bacterial biofilm formation is essential in wastewater treatment and food processing, and, in the context of the microbiome, commensal bacteria play roles in fending off invading pathogens, presumably by relying on quorum-sensing-controlled traits 12,13 . Indeed, anti- and pro-quorum-sensing compounds have been developed and show promise in relevant model systems 14–17 .…”

Section: Introductionmentioning

confidence: 99%

Surface-attached molecules control Staphylococcus aureus quorum sensing and biofilm development

et al. 2017

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Bacteria use a process called quorum sensing to communicate and orchestrate collective behaviors including virulence factor secretion and biofilm formation. Quorum sensing relies on production, release, accumulation, and population-wide detection of signal molecules called autoinducers. Here, we develop concepts to coat surfaces with quorum-sensing-manipulation molecules as a method to control collective behaviors. We probe this strategy using Staphylococcus aureus. Pro- and anti-quorum-sensing molecules can be covalently attached to surfaces using click chemistry, where they retain their abilities to influence bacterial behaviors. We investigate key features of the compounds, linkers, and surfaces necessary to appropriately position molecules to interact with cognate receptors, and the ability of modified surfaces to resist long-term storage, repeated infections, host plasma components, and flow-generated stresses. Our studies highlight how this surface approach can be used to make colonization-resistant materials against S. aureus and other pathogens and how the approach can be adapted to promote beneficial behaviors of bacteria on surfaces.

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“…Sentetik QS inhibitörleri de üzerine pek çok çalışmanın yapıldığı ve farklı moleküllerin geliştirildiği önemli tedavi seçenekleridir. Sentetik furanonlar ve metabromo-tiolakton P. aeruginosa'da in vivo etkinliği gösterilmiş moleküllerdir (68,69). Pnömokokların sebep olduğu koklear implant biyofilmi yapısına etkisi gösterilen "yd 47" yeni bir sentetik inhibitördür (70).…”

Section: Diyabetik Ayak Yaralarında Biyofilm Etkileriunclassified

Biofilm and Diabetic Foot Infections

Vatan¹,

Saltoğlu²

2017

Klimik Dergisi

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Biofilm is a well known issue with increasing importance, especially in the field of medicine. The biofilm formation is usually observed in foreign body infections as well as chronic wound infections. The diabetic foot ulcer is suitable for biofilm formation due to its rich wound exudate and debris content and impaired host immune response. The diabetic foot infection has emerged as one of the most important causes of mortality and morbidity in diabetes mellitus which needs special efforts to eradicate infection due to high rate of biofilm formation. In this review, we discuss the characteristic features of biofilms and the importance of the biofilm formation in diabetic foot infections. We also highlight the diagnosis and management of the biofilm formation.

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A quorum-sensing inhibitor blocks Pseudomonas aeruginosa virulence and biofilm formation

Cited by 659 publications

References 36 publications

Quorum sensing controls the Pseudomonas aeruginosa CRISPR-Cas adaptive immune system

Quorum sensing controls the Pseudomonas aeruginosa CRISPR-Cas adaptive immune system

Surface-attached molecules control Staphylococcus aureus quorum sensing and biofilm development

Biofilm and Diabetic Foot Infections

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