A radar-like DNA monitor for RNase H-targeted natural compounds screening and RNase H activity <i>in situ</i> detection

MicroRNA (miR)‐based therapy shows strong potential; however, structural limitations pose a challenge in fully exploiting its biomedical functionality. Tetrahedral framework DNA (tFNA) has proven to be an ideal vehicle for miR therapy. Inspired by the ancient Chinese myth “Sun and Immortal Birds,” a novel bioswitchable miR inhibitor delivery system (BiRDS) is designed with three miR inhibitors (the three immortal birds) and a nucleic acid core (the central sun). The BiRDS fuses miR inhibitors within the framework, maximizing their loading capacity, while allowing the system to retain the characteristics of small‐sized tFNA and avoiding uncertainty associated with RNA exposure in traditional loading protocols. The RNase H‐responsive sequence at the tail of each “immortal bird” enables the BiRDS to transform from a 3D to a 2D structure upon entering cells, promoting the delivery of miR inhibitors. To confirm the application potential, the BiRDS is used to deliver the miR‐31 inhibitor, with antiaging effects on hair follicle stem cells, into a skin aging model. Superior skin penetration ability and RNA delivery are observed with significant anti‐aging effects. These findings demonstrate the capability and editability of the BiRDS to improve the stability and delivery efficacy of miRs for future innovations.

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A Tetrahedral Framework DNA‐Based Bioswitchable miRNA Inhibitor Delivery System: Application to Skin Anti‐Aging

Wang

Zhang

et al. 2022

Advanced Materials

109

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A novel fluorescence amplification strategy combining cascade primer exchange reaction with CRISPR/Cas12a system for ultrasensitive detection of RNase H activity

Xie

Chen

Zhang

et al. 2022

Biosensors and Bioelectronics

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Biomimetic hybrid-cell membrane nanoparticles loaded with panaxytriol for breast cancer combinational therapy

Xie¹,

Li²,

Fan³

et al. 2022

Materials & Design

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A radar-like DNA monitor for RNase H-targeted natural compounds screening and RNase H activity in situ detection

Abstract: Ribonuclease H is an essential for the development and research of complex pathema. The high rigidity and versatility of DNA tetrahedrons are often used in biosensing systems. Inspired by "radar",...

Cited by 5 publications

References 31 publications

A Tetrahedral Framework DNA‐Based Bioswitchable miRNA Inhibitor Delivery System: Application to Skin Anti‐Aging

A Tetrahedral Framework DNA‐Based Bioswitchable miRNA Inhibitor Delivery System: Application to Skin Anti‐Aging

A novel fluorescence amplification strategy combining cascade primer exchange reaction with CRISPR/Cas12a system for ultrasensitive detection of RNase H activity

Biomimetic hybrid-cell membrane nanoparticles loaded with panaxytriol for breast cancer combinational therapy

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