A radial hemolysis method in agarose for the functional assay of properdin

Minta, Joe O.; Vetvutanapibul, W.

doi:10.1016/0022-1759(78)90175-8

Cited by 7 publications

(2 citation statements)

References 30 publications

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“…Despite the occurrence of this lag phase, certain complement activators are distinct activators of the alternative pathway, including guinea pig (4,5) and rabbit erythrocytes (6,7) and certain biomaterial surfaces (8,9).…”

mentioning

confidence: 99%

C3 Adsorbed to a Polymer Surface Can Form an Initiating Alternative Pathway Convertase

Andersson

Ekdahl

Larsson

et al. 2002

The Journal of Immunology

136

109

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Contact between blood and a biomaterial surface induces an immediate complement-mediated inflammatory response. Under these conditions, the alternative pathway of complement is often initiated and amplified on the biomaterial surface. Adsorption of a protein such as C3 to a polymer surface induces conformational changes in the protein. Based on the expression on adsorbed C3 of conformational neoepitopes specific for bound C3 fragments, we have hypothesized that adsorbed C3 is able to bind factor B and form a functional C3,Bb convertase. Using a quartz crystal microbalance to monitor binding of proteins to a polymer surface, we have demonstrated that a functional C3-containing alternative pathway convertase can be formed, in particular, in the presence of properdin. These data indicate that adsorption of C3 induces conformational changes that turn C3 into a C3b-like molecule that is able to participate in the functioning of the alternative convertase, and they suggest a new mechanism for complement activation on a biomaterial surface.

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mentioning

confidence: 99%

C3 Adsorbed to a Polymer Surface Can Form an Initiating Alternative Pathway Convertase

Andersson

Ekdahl

Larsson

et al. 2002

The Journal of Immunology

136

109

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“…The IgG, IgA, and IgM concentrations were all below 0.08 mg/ml and antibodies against P. carinii could not be demonstrated. Complement activity by the classical and the alternative pathway was determined using haemolysin – sensitized sheep erythrocytes and determination of the haemolytic activity of guinea‐pig erytrocytes, respectively (16, 17), and was found to be in the normal range.…”

Section: Methodsmentioning

confidence: 99%

Activation of the respiratory burst by Pneumocystis carinii

Laursen

Obel

Holmskov

et al. 2003

APMIS

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The effect of opsonization of Pneumocystis carinii with different antibody classes, complement, mannan-binding lectin (MBL), and lung surfactant protein D (SP-D) on respiratory burst activation was studied. Antibodies were obtained by affinity chromatography, complement from a hypogammaglobulinaemic patient, and phagocytic cells from blood donors. Respiratory burst activation was measured by chemiluminescence (CL). With freshly isolated neutrophils the combination of antibodies and complement but not antibody alone, had opsonizing properties. With neutrophils cultured for 20 h, however, IgG increased the CL response. In macrophages P. carinii opsonized with IgG alone induced a CL response proportional to the antibody titre used. With IgA an effect, albeit lower, was also seen, whereas IgM alone was inefficient. The combined effect of antibodies and complement increased the response significantly for all three antibody classes, IgG and complement giving the largest response. Binding of MBL to P. carinii and Candida albicans was demonstrated; however, only the former stimulated activation of the respiratory burst. SP-D did not bind to either microorganism and had no effect on the respiratory burst. It is concluded that IgG, IgA and complement are important opsonizing factors in infections involving P. carinii. The relative importance varies with the type of phagocytic cell studied.

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