A radiofrequency coil to facilitate task-based fMRI of awake marmosets

A vocalization-processing network in marmosets

“…Marmosets were placed inside the MRI tube in which an adjustable collar and tail plate held the animal in a sphinx position. After performing the head fixation steps described (25), MRI-compatible auditory tubes (S14, Sensimetrics, Gloucester, MA) were directly placed into the animals’ ear canals bilaterally and were fixed using reusable sound-attenuating silicone earplugs (Amazon) and self-adhesive veterinary bandage as indicated in Fig.1a. When the animal was placed within the bore, a small black circle (1.5 cm in diameter) on the center of a gray solid background was presented on a translucent plexiglass screen located at a distance of 120 cm from marmosets’ eyes and reflected through a wall-mounted mirror as a fixation point to mitigate the effects of nystagmus induced by vestibular magnetic stimulation throughout a whole run (55).…”

“…Marmosets were placed inside the MRI tube in which an adjustable collar and tail plate held the animal in a sphinx position. After performing the head fixation steps described (25), MRI-compatible auditory tubes (S14, Sensimetrics, Gloucester, MA) were directly placed into the animals' ear canals bilaterally and were fixed using reusable sound-attenuating silicone earplugs (Amazon) and self-adhesive veterinary bandage as indicated in Fig. 1a.…”

“…Single unit recordings have demonstrated vocalization-selective responses in marmoset auditory (19)(20)(21) and frontal cortices (22,23) and an fMRI study has reported vocalization-selective activation in the anterior temporal pole in anesthetized marmosets (24). Here, we took advantage of our recently developed radiofrequency (RF) coil and restraint system for ultrahigh field (9.4 Tesla) fMRI in awake marmosets (25) and acquired whole-brain fMRI while monkeys were presented with conspecific vocalizations, scrambled vocalizations, and nonvocal sounds. To investigate the functional connectivity of vocalization-selective clusters, we utilized a fully awake resting-state fMRI (RS-fMRI) dataset (26).…”

Gaze patterns and brain activations in humans and marmosets in the Frith-Happé theory-of-mind animation task

“…Twelve adult marmosets (7 females, 32-57 months, mean age: 36.6 months) were subjects in this study. All animals were implanted for head-fixed experiments with either a fixation chamber (Johnston et al, 2018) or a head post (Gilbert et al, 2023) under anesthesia and aseptic conditions. Briefly, the animals were placed in a stereotactic frame (Narishige, model SR-6C-HT) while being maintained under gas anaesthesia with a mixture of O 2 and air (isoflurane 0.5-3%).…”

“…Twelve adult marmosets (7 females, 32-57 months, mean age: 36.6 months) were subjects in this study. All animals were implanted for head-fixed experiments with either a fixation chamber (Johnston et al, 2018) or a head post (Gilbert et al, 2023) under anesthesia and aseptic conditions.…”