A radioimmunoassay for abscisic acid

Walton, Daniel C.; Dashek, William V.; Galson, Eva C.

doi:10.1007/bf00388224

Cited by 57 publications

(31 citation statements)

References 23 publications

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“…2). This condition was in accordance with other reports (24,25) and resulted in a diminished RIA sensitivity and precision.…”

supporting

confidence: 93%

“…A linear logit-log plot of the ABA-methyl ester standard curve is presented in Figure 4. The measuring range ofthe assay compares favorably with other published immunoassays (I13, 20,[23][24][25][26] from 0.1 to 50 pmol of (+)-ABA. Slope of the logit-log plot, an indicator of the assay precision, equals -0.69, a value lower than that for RIA (26), but comparable to the EIA reported by Weiler (27 (Fig.…”

supporting

confidence: 59%

“…These materials were assumed to represent either degradation products or reaction products attributable to ether peroxides. Similar problems apparently linked with the use of diethyl ether in RIA have also been noted (24). The choice of methylene chloride as an alternative solvent was based on its comparably high partition coefficient for ABA (24) and low partition coefficient for ABA-GE (18) under the conditions described.…”

mentioning

confidence: 76%

“…Similar problems apparently linked with the use of diethyl ether in RIA have also been noted (24). The choice of methylene chloride as an alternative solvent was based on its comparably high partition coefficient for ABA (24) and low partition coefficient for ABA-GE (18) under the conditions described. The anomalous materials originally observed with either partitioning were not observed when methylene chloride was used.…”

mentioning

confidence: 76%

“…Several recent advances in immunological methods have provided attractive alternatives to the traditional assays for these compounds (12,21,(24)(25)(26)(27). These assays minimize the need for purification and allow for rapid analyses of many samples using little plant material.…”

mentioning

confidence: 99%

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The Occurrence of Abscisic Acid and Abscisyl-β-d-Glucopyranoside in Developing and Mature Citrus Fruit as Determined by Enzyme Immunoassay

Harris

Dugger²

1986

Plant Physiol.

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The contents of (+)-cis-abscisic acid (ABA) and alkaline-hydrolyzable ABA-conjugate(s) ABA and water-soluble ABA-conjugates are important components of the growth inhibitor complex of many plants (1). The levels of these compounds have been reported to increase during fruit maturation and this observation has given rise to speculation on their role in fruit ripening and other related developmental phenomena (16). Increases in ABA and ABA-conjugates in citrus fruit have been related to exocarp senescence and the transition of chloroplasts to chromoplasts that occurs in this tissue (6,7,23). Accumulation of ABA and possibly the 2,trans-isomer of ABA in fruit has been postulated to influence bud dormancy and contributed to biennial-bearing which is common in many citrus varieties (5, 10). Numerous reports on the endogenous level of growth inhibitors in citrus fruit have appeared in the literature (see Ref. 4 for review). For the most part, these reports have relied upon relatively nonspecific bioassays for the estimation of ABA. Although reports using GC or GC-MS have also appeared, the requirements for sample prepurification make routine analyses difficult by these methods. Furthermore, the variable sample loss incurred ' Supported from funds provided by the

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“…2). This condition was in accordance with other reports (24,25) and resulted in a diminished RIA sensitivity and precision.…”

supporting

confidence: 93%