A radioimmunoassay for immunologic phenomena in pneumococcal disease and for the antibody response to pneumococcal vaccines. I. Method for the radioimmunoassay of anticapsular antibodies and comparison with other techniques

Schiffman, Gerald; Douglas, Robert M.; Bonner, Mary J.; Robbins, Miriam; Austrian, Robert

doi:10.1016/s0022-1759(80)80004-4

Cited by 186 publications

(71 citation statements)

References 20 publications

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“…The original assays used to quantify the level of circulating antibodies to pneumococcal capsular PS were based on the Farr assay, a radioimmunoassay that measures antibody binding to radiolabeled capsular PS (56). However, the Farr assay is impractical to support assessment of thousands of specimens associated with clinical trials.…”

Section: Historymentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay for Quantitation of Human Antibodies to Pneumococcal Polysaccharides

Wernette

Frasch

Madore

et al. 2003

Clin Vaccine Immunol

291

222

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Section: Historymentioning

confidence: 99%

Enzyme-Linked Immunosorbent Assay for Quantitation of Human Antibodies to Pneumococcal Polysaccharides

Wernette

Frasch

Madore

et al. 2003

Clin Vaccine Immunol

291

222

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“…Antibody to the other polysaccharides was quantitated by radioimmunoassay using radiolabeled antigens from 11 pneumococcal vaccine types (22) in Dr. Schiffman's laboratory, and meningococcal serogroups A and C (23) in Dr. Gotschlich's laboratory.…”

Section: Introductionmentioning

confidence: 99%

Correlation between G2m(n) immunoglobulin allotype and human antibody response and susceptibility to polysaccharide encapsulated bacteria.

Ambrosino¹,

Schiffman²,

Gotschlich³

et al. 1985

J. Clin. Invest.

169

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To determine whether genetic factors influence the human antibody response to polysaccharides, we correlated Ig allotypes with the concentrations of antibody to 14 bacterial capsular antigens in 130 actively immunized Caucasian adults. The 88 individuals possessing G2m(n), an allotype antigen of IgG2 subclass heavy chains, had significantly higher postimmunization antibody levels to Haemophilus influenzae type b (Hib) and 8 of 11 pneumococcal types (P < 0.05) than the 42 lacking this antigen. For Hib, pneumococcus type 14, and meningococcus group C, an increased response was observed in IgG class but not in IgM or IgA classes of antibody. The G2m(n) positive individuals also had higher preimmunization antibody levels to most polysaccharide antigens. Total IgG2 concentrations were correlated with the mean postimmunization antibody concentrations to pneumococci (P = 0.005), but this correlation was independent of G2m(n) by multiple regression analysis.To determine if the lack of G2m(n) was associated with increased susceptibility to infection, we compared the frequencies of various Ig allotypes in 98 children infected with Hib and 98 matched controls. Caucasian children with Hib infections other than epiglottitis were significantly more likely to lack the G2m(n) allotype than controls (P < 0.05). G2m(n) negative Caucasian children . 18 mo old have a 5

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“…IgG and IgM antibodies to S. flexneri type 2a lipopolysaccharide (LPS) and to E. coli K1 polysaccharides were measured by enzymelinked immunosorbent assay (ELISA) as described previously (8,12). IgG antipneumococcal type 14 polysaccharide was assayed by ELISA, and total polysaccharide Ab was assayed by radioimmunoassay (RIA) as described previously (18,36,37).…”

mentioning

confidence: 99%

Clostridium difficile Recombinant Toxin A Repeating Units as a Carrier Protein for Conjugate Vaccines: Studies of Pneumococcal Type 14, Escherichia coli K1, and Shigella flexneri Type 2a Polysaccharides in Mice

et al. 2000

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Unlike the native protein, a nontoxic peptide (repeating unit of the native toxin designated rARU) from Clostridium difficile toxin A (CDTA) afforded an antigen that could be bound covalently to the surface polysaccharides of pneumococcus type 14, Shigella flexneri type 2a, and Escherichia coli K1. The yields of these polysaccharide-protein conjugates were significantly increased by prior treatment of rARU with succinic anhydride. Conjugates, prepared with rARU or succinylated (rARUsucc), were administered to mice by a clinically relevant dosage and immunization scheme. All conjugates elicited high levels of serum immunoglobulin G both to the polysaccharides and to CDTA. Conjugate-induced anti-CDTA had neutralizing activity in vitro and protected mice challenged with CDTA, similar to the rARU alone. Conjugates prepared with succinylated rARU, therefore, have potential for serving both as effective carrier proteins for polysaccharides and for preventing enteric disease caused by C. difficile.

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A radioimmunoassay for immunologic phenomena in pneumococcal disease and for the antibody response to pneumococcal vaccines. I. Method for the radioimmunoassay of anticapsular antibodies and comparison with other techniques

Cited by 186 publications

References 20 publications

Enzyme-Linked Immunosorbent Assay for Quantitation of Human Antibodies to Pneumococcal Polysaccharides

Enzyme-Linked Immunosorbent Assay for Quantitation of Human Antibodies to Pneumococcal Polysaccharides

Correlation between G2m(n) immunoglobulin allotype and human antibody response and susceptibility to polysaccharide encapsulated bacteria.

Clostridium difficile Recombinant Toxin A Repeating Units as a Carrier Protein for Conjugate Vaccines: Studies of Pneumococcal Type 14, Escherichia coli K1, and Shigella flexneri Type 2a Polysaccharides in Mice

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