A ramie (Boehmeria nivea) bZIP transcription factor BnbZIP3 positively regulates drought, salinity and heavy metal tolerance

Huang, Chengjian; Zhou, Jia; Jie, Yucheng; Xing, Hucheng; Zhong, Yaohua; She, Wei; Wei, Gang; Yu, Weilin; Ma, Yushen

doi:10.1007/s11032-016-0470-2

Cited by 32 publications

(23 citation statements)

References 43 publications

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“…Mutants having non-functional ABA bio-synthesis are extra vulnerable to environment deviations when compared to transgenic plants which can induce much hormonal response and forecast higher tolerance against abiotic stress than the wild type ( Iuchi et al, 2001 ; Qin and Zeevaart, 2002 ). Huang et al (2016) studied that ramie BnbZIP3 gene was upregulated in the presence of drought, high saline and ABA in ramie. BnbZIP3 gene belongs to the group of bZIP transcription factors.…”

Section: Aba and Abiotic Stress Signalingmentioning

confidence: 99%

Abscisic Acid Signaling and Abiotic Stress Tolerance in Plants: A Review on Current Knowledge and Future Prospects

et al. 2017

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Abiotic stress is one of the severe stresses of environment that lowers the growth and yield of any crop even on irrigated land throughout the world. A major phytohormone abscisic acid (ABA) plays an essential part in acting toward varied range of stresses like heavy metal stress, drought, thermal or heat stress, high level of salinity, low temperature, and radiation stress. Its role is also elaborated in various developmental processes including seed germination, seed dormancy, and closure of stomata. ABA acts by modifying the expression level of gene and subsequent analysis of cis-and trans-acting regulatory elements of responsive promoters. It also interacts with the signaling molecules of processes involved in stress response and development of seeds. On the whole, the stress to a plant can be susceptible or tolerant by taking into account the coordinated activities of various stress-responsive genes. Numbers of transcription factor are involved in regulating the expression of ABA responsive genes by acting together with their respective cis-acting elements. Hence, for improvement in stresstolerance capacity of plants, it is necessary to understand the mechanism behind it. On this ground, this article enlightens the importance and role of ABA signaling with regard to various stresses as well as regulation of ABA biosynthetic pathway along with the transcription factors for stress tolerance.

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Section: Aba and Abiotic Stress Signalingmentioning

confidence: 99%

Abscisic Acid Signaling and Abiotic Stress Tolerance in Plants: A Review on Current Knowledge and Future Prospects

et al. 2017

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“…It was predicted that N assimilation would be enhanced as a method of supplying N demands under N stress condition [ 46 ]. The role of some key transcription factors, including MYB ((v-myb avian myeloblastosis viral oncogene homolog), AP2/ERF (APETALA2/ethylene responsive factor), bZIP (basic leucine zipper), Dof (DNA Binding with one finger), etc., involved in drought or N-deficit stress responses, has been identified in ramie [ 20 , 47 , 48 , 49 ]. Previous studies also clarified the role of some genes involved in drought/N-deficit responses in ramie, including sucrose synthase genes, phloem protein genes, and glutamine synthetase gens [ 50 , 51 , 52 ].…”

Section: Discussionmentioning

confidence: 99%

Integrative Transcriptome and Proteome Analysis Identifies Major Molecular Regulation Pathways Involved in Ramie (Boehmeria nivea (L.) Gaudich) under Nitrogen and Water Co-Limitation

Chen

Gao

Chen

et al. 2020

Plants

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Water and N are the most important factors affecting ramie (Boehmeria nivea (L.) Gaudich) growth. In this study, de novo transcriptome assembly and Tandem Mass Tags (TMT) based quantitative proteome analysis of ramie under nitrogen and water co-limitation conditions were performed, and exposed to treatments, including drought and N-deficit (WdNd), proper water but N-deficit (WNd), proper N but drought (WdN), and proper N and water (CK), respectively. A total of 64,848 unigenes (41.92% of total unigenes) were annotated in at least one database, including NCBI non-redundant protein sequences (Nr), Swiss-Prot, Protein family (Pfam), Gene Ontology (GO) and KEGG Orthology (KO), and 4268 protein groups were identified. Most significant changes in transcript levels happened under water-limited conditions, but most significant changes in protein level happened under water-limited conditions only with proper N. Poor correlation between differentially expressed genes (DEGs) and differentially expressed proteins (DEPs) was observed in ramie responding to the treatments. DEG/DEP regulation patterns related to major metabolic processes responding to water and N deficiency were analyzed, including photosynthesis, ethylene responding, glycolysis, and nitrogen metabolism. Moreover, 41 DEGs and 61 DEPs involved in regulating adaptation of ramie under water and N stresses were provided in the study, including DEGs/DEPs related to UDP—glucuronosyhransferase (UGT), ATP synthase, and carbonate dehydratase. The strong dependency of N-response of ramie on water conditions at the gene and protein levels was highlighted. Advices for simultaneously improving water and N efficiency in ramie were also provided, especially in breeding N efficient varieties with drought resistance. This study provided extensive new information on the transcriptome, proteome, their correlation, and diversification in ramie responding to water and N co-limitation.

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“…while BnbZIP3, a ramie (Boehmeria nivea) bZIP transcription factor, also increased the drought, salinity, and heavy metal tolerances of transgenic Arabidopsis (Huang et al, 2016). These genes are also involved in the regulation of other processes; for example, the repression of a bZIP transcription factor gene OsABI5 expression in rice resulted in low fertility (Zou et al, 2008),…”

Section: Discussionmentioning

confidence: 99%

Peer Review #1 of "Investigation for a multi-silique trait in Brassica napus by alternative splicing analysis (v0.1)"

Liu¹

2020

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Background. Flower and fruit development are vital stages of the angiosperm lifecycle. We previously investigated the multi-silique trait in the rapeseed (Brassica napus) line zws-ms on a genomic and transcriptomic level, leading to the identification of two genomic regions and several candidate genes associated with this trait. However, some events on transcriptome level, like alternative splicing, were poorly understood. Methods. Plants from zws-ms and its near-isogenic line (NIL) zws-217 were both grown in Xindu with normal conditions and a colder area Ma'erkang. Buds from the two lines were sampled and RNA was isolated to perform the transcriptomic sequencing. The numbers and types of alternative splicing (AS) events from the two lines were counted and classified. Genes with AS events and expressed differentially between the two lines, as well as genes with AS events which occurred in only one line were emphasized. Their annotations were further studied. Results. From the plants in Xindu District, an average of 205,496 AS events, which could be sorted into 5 AS types, were identified. zws-ms and zws-217 shared highly similar ratios of each AS type: The alternative 5' and 3' splice site types were the most common, while the exon skipping type was observed least often. Eleven differentially expressed AS genes were identified, of which four were upregulated and seven were downregulated in zws-ms. Their annotations implied that five of these genes were directly associated with the multi-silique trait. While samples from colder area Ma'erkang generated generally reduced number of each type of AS events except the Intron Retention; but the number of differentially expressed AS genes increased significantly. Further analysis found that among the 11 differentially expressed AS genes from Xindu, three of them maintained the same expression models, while the other 8 genes did not show significant difference between the two lines in expression level. Additionally, the 205 line-specifically expressed AS genes were analyzed, of which 187 could be annotated, and two were considered to be important. Discussion. This study provides new insights into the molecular mechanism of the agronomically important multi-silique trait in rapeseed on transcriptome level and screens out some environmentresponding candidate genes.

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A ramie (Boehmeria nivea) bZIP transcription factor BnbZIP3 positively regulates drought, salinity and heavy metal tolerance

Cited by 32 publications

References 43 publications

Abscisic Acid Signaling and Abiotic Stress Tolerance in Plants: A Review on Current Knowledge and Future Prospects

Abscisic Acid Signaling and Abiotic Stress Tolerance in Plants: A Review on Current Knowledge and Future Prospects

Integrative Transcriptome and Proteome Analysis Identifies Major Molecular Regulation Pathways Involved in Ramie (Boehmeria nivea (L.) Gaudich) under Nitrogen and Water Co-Limitation

Peer Review #1 of "Investigation for a multi-silique trait in Brassica napus by alternative splicing analysis (v0.1)"

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