A randomized double-blind controlled study of the efficacy of laser-assisted hatching on implantation and pregnancy rates of frozen–thawed embryo transfer at the cleavage stage

Ng, Ernest Hung Yu; Naveed, Fatima; Lau, Esther Yuet Ying; Yeung, William Shu Biu; Chan, Chung-hong; Tang, Oi Shan; Ho, Pak Chung

doi:10.1093/humrep/deh724

Cited by 61 publications

(60 citation statements)

References 30 publications

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“…However, to the best of our knowledge, no single study has been able to demonstrate sufficient evidence favourable to AH, and the current research conclusions are not unanimous (6)(7)(8)(9)(10)(11)(12)(13). In a large meta-analysis, Martins et al showed that laser-AH (LAH) is currently one of the best, safest and most effective AH methods (14).…”

Section: Introductionmentioning

confidence: 83%

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Wang¹,

Wang

et al. 2016

Biomedical Reports

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Abstract. The aim of the present study was to determine whether the size of the zona pellucida (ZP) thinning area by laser-assisted hatching affected the potential development of vitrified-warmed embryos. A total of 196 vitrified-warmed cleavage-stage embryos (from 49 patients, four sister embryos per patient) were used in the study, i.e., four sister embryos from each patient were randomly assigned to four groups: A control group of embryos that were not zona-manipulated (zona intact, group A); one experimental group of embryos in which a quarter of the zona pellucida was thinned using laser-assisted ZP thinning (group B); a second experimental group of embryos in which half of ZP was thinned (group C); and a third group in which two-thirds of the ZP was thinned (group D). Subsequent blastocyst development was assessed. Microscopy was performed to study the hatching process of the embryos after zona thinning. The blastocyst formation rates were 71.43% in group A, 67.35% in group B, 65.31% in group C, and 51.02% in group D (groups B-D vs. group A, P=0.661, P=0.515, P= 0.038, respectively). The rates of complete hatching were 30.61% in group A, 38.78% in group B, 61.22% in group C, and 48.98% in group D (groups B-D vs. group A, P= 0.396, P= 0.002, P= 0.063, respectively). For a subgroup of patients, there was a significant difference in the complete hatching in all the groups for women aged <35 years (P=0.011), and there was a significant difference in the complete hatching in all the groups for secondary infertility women (P=0.022). There was no significant difference in the blastocyst formation rates in the different groups of women aged ≥35 years (P= 0.340). In addition, there was no significant difference in the complete hatching in the different groups among women aged ≥35 years (P= 0.492). The results of the present study showed that in vitrified-warmed embryo transfers at the cleavage-stage, and the two-thirds zona pellucida thinning group demonstrated a significantly decreased blastocyst formation rate compared with the control group, while the half zona pellucida thinning group demonstrated a significantly increased complete hatching rate compared with the control group, which may have a high value in clinical application.

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Section: Introductionmentioning

confidence: 83%

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Wang¹,

Wang

et al. 2016

Biomedical Reports

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“…Laser AH for frozen cleaved embryos is normally performed at the cleavage stage [3,6]. In this study, we performed laser AH for frozen cleaved embryos at the blastocyst stage.…”

Section: Discussionmentioning

confidence: 99%

“…assisted hatching (AH), may be useful for frozen thawed embryo transfer. However, very recent studies focused on the benefit of AH following frozen thawed embryo transfer reported controversial results with decreased [2], similar [3,4] or increased [5,6] implantation rates in the AH group compared to the control group. Different types of AH have been developed.…”

Section: Introductionmentioning

confidence: 99%

Effect of the size of zona pellucida opening by laser assisted hatching on clinical outcome of frozen cleaved embryos that were cultured to blastocyst after thawing in women with multiple implantation failures of embryo transfer: a retrospective study

Fuchiwaki

Hiraoka

Horiuchi

et al. 2008

J Assist Reprod Genet

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“…Contrary to all these reports, Sifer et al [24] indicated that the partial enzymatic digestion of ZP by pronase was not related to any benefit of the frozenthawed embryo transfer outcome, especially concerning the implantation ability of frozen-thawed embryos (N-AH, 9.6% vs AH, 9.2%) and clinical pregnancy (N-AH, 18.0% vs AH, 17.2%). In a study, Ng et al [25] found a negative effect of AH on frozen-thawed embryos. Matson et al [26] also suggested that cryopreservation of embryos is not associated with zona hardening or reducing implantation, making microdissection of the zona in such cases generally unwarranted.…”

Section: Discussionmentioning

confidence: 98%

Effect of laser-assisted hatching and necrotic blastomere removal on the development of vitrified–warmed four-cell mouse embryos

Fathi

Valojerdi

Eftekhari‐Yazdi

2008

J Assist Reprod Genet

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Purpose To investigate the effect of laser-assisted hatching and necrotic blastomere removal on the development of vitrified-warmed mouse embryos. Methods The vitrified-warmed four-cell stage mouse embryos were divided into five groups; vitrified intact with no laser-assisted hatching, vitrified intact with laser-assisted hatching, vitrified damaged with neither laser assisted hatching nor necrotic blastomere removal, vitrified damaged with laser-assisted hatching, and vitrified damaged with necrotic blastomere removal. Thereafter blastocyst formation, blastomere and apoptotic cell number within all groups were statistically compared. Results The rate of blastocyst formation showed a significant improvement in the group vitrified intact with laserassisted hatching. However, neither laser-assisted hatching nor necrotic blastomere removal can improve a delayed vitrified-warmed damaged embryos in term of blastocyst formation and total cell number. Nevertheless, apoptotic cell number was significantly reduced after application of both techniques.Conclusions Laser-assisted hatching can improve the development of vitrified-warmed intact four-cell stage mouse embryos, whereas necrotic blastomere removal has no significant effect on the development of vitrified-warmed four-cell stage damaged embryos.

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A randomized double-blind controlled study of the efficacy of laser-assisted hatching on implantation and pregnancy rates of frozen–thawed embryo transfer at the cleavage stage

Abstract: Laser AH did not improve the implantation rate of FET cycles and should not be performed routinely in all frozen-thawed embryos at the cleavage stage.

Cited by 61 publications

References 30 publications

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Outcomes of vitrified-warmed cleavage-stage embryo hatching after in vitro laser-assisted zona pellucida thinning in patients

Effect of the size of zona pellucida opening by laser assisted hatching on clinical outcome of frozen cleaved embryos that were cultured to blastocyst after thawing in women with multiple implantation failures of embryo transfer: a retrospective study

Effect of laser-assisted hatching and necrotic blastomere removal on the development of vitrified–warmed four-cell mouse embryos

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