A rapid and efficient technique for the isolation of Bacillus genomic DNA using a cocktail of peptidoglycan hydrolases of different type

Chernyshov, Sergei V.; Tsvetkova, Diana V; Mikoulinskaia, Galina V.

doi:10.1007/s11274-022-03475-2

Search citation statements

Order By: Relevance

Paper Sections

Select...

Citation Types

Supporting

Mentioning

Contrasting

Year Published

2024

2025

Publication Types

Select...

Article3

Preprint1

Relationship

Self Cite1

Independent3

Authors

Journals

Cited by 4 publications

References 30 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

Thread-powered cell lysis and isotachophoresis: unlocking microbial DNA for diverse molecular applications

Garg,

Maurya,

Mani

et al. 2024

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Thread-powered cell lysis and isotachophoresis: unlocking microbial DNA for diverse molecular applications

Garg,

Maurya,

Mani

et al. 2024

World J Microbiol Biotechnol

View full text Add to dashboard Cite

Deep dive into the diversity and properties of rhodopsins in actinomycetes of the family Geodermatophilaceae

Tarlachkov,

Starodumova,

Boueva

et al. 2025

Journal of Photochemistry and Photobiology B: Biology

View full text Add to dashboard Cite

Deep dive into the diversity and properties of rhodopsins in actinomycetes of the familyGeodermatophilaceae

Tarlachkov,

Starodumova,

Boueva

et al. 2024

Preprint

Self Cite

View full text Add to dashboard Cite

Rhodopsin with the NTQ motif and heliorhodopsins, both discovered for the first time in actinomycetes of the family Geodermatophilaceae were characterized, along with characterization of the DTEW, DTEF and NDQ rhodopsins reported in members of this family previously. The identified absorption maxima ranged from 513 to 559 nm, of which the blue-shifted rhodopsin contained the NTQ motif and the red-shifted one were heliorhodopsin. An assessment of the pumping activity of the NTQ rhodopsin and heliorhodopsin showed that the former functioned as inward Cl--, Br--, I--pump with much weaker activity towards NO3-, while no any activity was detected for the later. The DTEW and DTEF rhodopsins had the outward H+-transport activity which was dependent on the presence of Ca2+ ions and on the particular E. coli expression strain. The outward pumping of H+ was also detected for NDQ rhodopsin both in NaCl and KCl solutions at pH 5 and 6, but not at pH 7. Weak Na+-efflux activity for this rhodopsin was observed at pH 6 and 7 in NaCl solution only after adding carbonyl cyanide m-chlorophenyl hydrazone (CCCP).

show abstract

A rapid and efficient technique for the isolation of Bacillus genomic DNA using a cocktail of peptidoglycan hydrolases of different type

Cited by 4 publications

References 30 publications

Thread-powered cell lysis and isotachophoresis: unlocking microbial DNA for diverse molecular applications

Thread-powered cell lysis and isotachophoresis: unlocking microbial DNA for diverse molecular applications

Deep dive into the diversity and properties of rhodopsins in actinomycetes of the family Geodermatophilaceae

Deep dive into the diversity and properties of rhodopsins in actinomycetes of the familyGeodermatophilaceae

Contact Info

Product

Resources

About