A Rapid and Highly Efficient Method for Transformation of Sugarcane Callus

Santosa, Dwi Andreas; Hendroko, Roy; Farouk, Abd‐ElAziem; Greiner, Ralf

doi:10.1385/mb:28:2:113

Cited by 14 publications

(7 citation statements)

References 7 publications

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“…In general, the microbial phytase-encoding gene is mainly derived from Aspergillus niger, Bacillus subtilis, Aspergillus fumigatus, Escherichia coli, and Schwanniomyces occidentalis (Greiner & Konietzny, 2006). The transgenesis has been carried out in rice (Hamada et al, 2005), wheat (Brinch-Pedersen, Olesen, Rasmussen, & Holm, 2000), sugarcane (Santosa, Hendroko, Farouk, & Greiner, 2004), alfalfa (Ullah, Sethumadhavan, Mullaney, Ziegelhoffer, & Austin-Phillips, 2002), arabidopsis (Mudge, Smith, & Richardson, 2003), sesame (Jin et al, 2004), soybean (Li et al, 1997), canola (Ponstein et al, 2002) and potato (Ullah, Sethumadhavan, Mullaney, Ziegelhoffer, & Austin-Phillips, 2003). For human nutrition applications, only one transgenic plant expressing phytase from Aspergillus fumigatus gene has been developed (Lucca, Hurrell, & Potrykus, 2001).…”

Section: Sources Of Phytasementioning

confidence: 99%

Dietary roles of phytate and phytase in human nutrition: A review

et al. 2010

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Section: Sources Of Phytasementioning

confidence: 99%

Dietary roles of phytate and phytase in human nutrition: A review

et al. 2010

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“…Thus for most of these grasses, the exception being maize, major progress is required in the development and optimization of transformation protocols. Reviews on the status of transformation of sorghum (Howe et al, 2006; Girijashankar and Swathisree, 2009), switchgrass (Somleva et al, 2002; Conger, 2003; Bouton, 2007; Burris et al, 2009; Xi et al, 2009; Saathoff et al, 2011), miscanthus (Wang et al, 2011; Engler and Jakob, 2013) and sugarcane (Santosa et al, 2004; Hotta et al, 2010) provide further information. However, transgenic approaches are regarded with great caution in dedicated bioenergy crops as well, as they are mostly outcrossing perennial grasses (Wang and Brummer, 2012).…”

Section: Genetic Improvementmentioning

confidence: 99%

The potential of C4 grasses for cellulosic biofuel production

Weijde¹,

Kamei²,

Torres³

et al. 2013

Front. Plant Sci.

170

134

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† These authors have contributed equally to this work.With the advent of biorefinery technologies enabling plant biomass to be processed into biofuel, many researchers set out to study and improve candidate biomass crops. Many of these candidates are C4 grasses, characterized by a high productivity and resource use efficiency. In this review the potential of five C4 grasses as lignocellulosic feedstock for biofuel production is discussed. These include three important field crops-maize, sugarcane and sorghum-and two undomesticated perennial energy grasses-miscanthus and switchgrass. Although all these grasses are high yielding, they produce different products. While miscanthus and switchgrass are exploited exclusively for lignocellulosic biomass, maize, sorghum, and sugarcane are dual-purpose crops. It is unlikely that all the prerequisites for the sustainable and economic production of biomass for a global cellulosic biofuel industry will be fulfilled by a single crop. High and stable yields of lignocellulose are required in diverse environments worldwide, to sustain a year-round production of biofuel. A high resource use efficiency is indispensable to allow cultivation with minimal inputs of nutrients and water and the exploitation of marginal soils for biomass production. Finally, the lignocellulose composition of the feedstock should be optimized to allow its efficient conversion into biofuel and other by-products. Breeding for these objectives should encompass diverse crops, to meet the demands of local biorefineries and provide adaptability to different environments. Collectively, these C4 grasses are likely to play a central role in the supply of lignocellulose for the cellulosic ethanol industry. Moreover, as these species are evolutionary closely related, advances in each of these crops will expedite improvements in the other crops. This review aims to provide an overview of their potential, prospects and research needs as lignocellulose feedstocks for the commercial production of biofuel.

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“…However, Agrobacterium-mediated transformation provides some advantages in comparison with direct transformation methods, such as: (i) simple, economical and efficient procedure; (ii) integration of low copy numbers of the gene into the plant chromosomes; and (iii) transfer of relatively large segments of DNA with little rearrangement (Wei et al 2003;Manickavasagam et al 2004;Santosa et al 2004).…”

Section: Introductionmentioning

confidence: 99%

Improved Agrobacterium-mediated genetic transformation of GNA transgenic sugarcane

et al. 2007

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Six plasmids carrying a snowdrop lectin (Galanthus nivalis agglutinin, GNA) and one of three selection markers were successfully transferred into two sugarcane cultivars (FN81-745 and Badila) via Agrobacterium-mediated transformation. Agrobacterium strains LBA4404, EHA105 and A281 that harboured a super-binary vector were used for sugarcane transformation. The use of the hygromycin (Hyg) resistance gene (hpt II), phosphinothrincin (PPT) resistance gene (bar) or G418 resistance gene (npt II) as a screenable marker facilitated the initial selection of GNA transgenic sugarcane callus with different efficiencies and helped the rapid segregation of individual transformation events. All the three selective marker genes were controlled by CaMV 35S promoter, while GNA gene was controlled by promoter of RSs-1 (rice sucrose synthase-1) or Ubi (maize ubiquitin). Factors important to successful transformation mediated by Agrobacterium tumefaciens were optimized, which included concentration of A. tumefaciens, medium composition, co-cultivated methods with plant tissue, strain virulence and different selective marker genes. An efficient protocol for sugarcane transformation mediated by A. tumefaciens was established. The GNA gene has been integrated into sugarcane genome as demonstrated by PCR and Southern dot blotting detections. The preliminary results from bioassay demonstrated a significant resistance of the transgenic sugarcane plants to woolly aphid (Ceratovacuna lanigera Zehnther) indicating thus the possibility for obtaining a transgenic sugarcane cultivar with resistance to woolly aphid.

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A Rapid and Highly Efficient Method for Transformation of Sugarcane Callus

Cited by 14 publications

References 7 publications

Dietary roles of phytate and phytase in human nutrition: A review

Dietary roles of phytate and phytase in human nutrition: A review

The potential of C4 grasses for cellulosic biofuel production

Improved Agrobacterium-mediated genetic transformation of GNA transgenic sugarcane

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