A Rapid and Quantitative Detection System for &lt;i&gt;Streptococcus mutans&lt;/i&gt; in Saliva Using Monoclonal Antibodies

Matsumoto, Yoshiko; Sugihara, Naoki; Koseki, Masamichi; Maki, Yoshinobu

doi:10.1159/000088900

Cited by 19 publications

(13 citation statements)

References 18 publications

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“… Total microorganisms in saliva: 17 Evaluation was based on three categories of low, middle and high by using the RD test (SHOWA YAKUHIN KAKO, Tokyo, Japan). Lactobacilli in saliva: 18 Dentocult LB (Orion Diagnostica, Espoo, Finland) was used. The level of Lactobacilli was determined as 0: 10 3 /mL or less; 1: 10 4 /mL; 2: 10 5 /mL; and 3: 10 6 /mL or more. Streptococcus mutans in saliva: 19 Level of S. mutans was measured by using SALIVA‐CHECK MUTANS (GC, Tokyo, Japan). The S. mutans level per 1 mL saliva was determined as 1: less than 10 5 /mL; 2: 10 5 /mL; and 3: 10 6 /mL and over. Candida in saliva: 20 Candida was detected and the level measured after cultivation for 4 days using CHROMO Slant Candida Color (KANTO CHEMICAL, Tokyo, Japan). …”

Section: Methodsmentioning

confidence: 99%

Evaluation of a Japanese “Prevention of Long‐term Care” project for the improvement in oral function in the high‐risk elderly

Sakayori

Maki

Hirata

et al. 2012

Geriatrics Gerontology Int

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Section: Methodsmentioning

confidence: 99%

Evaluation of a Japanese “Prevention of Long‐term Care” project for the improvement in oral function in the high‐risk elderly

Sakayori

Maki

Hirata

et al. 2012

Geriatrics Gerontology Int

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“…The presence of Streptococcus mutans was assessed using a chairside diagnostic kit which has a lower limit of detection of 1 ! 10 4 bacteria/ml [Matsumoto et al, 2006] (Saliva-Check Mutans Test; GC Corporation, Tokyo, Japan). Briefly, all the teeth were swabbed using a sponge-tipped swab supplied by the manufacturer, and the swab saturated with saliva by placing the swab underneath the tongue.…”

Section: Subjectsmentioning

confidence: 99%

Case-Control Study of Early Childhood Caries in Australia

Seow¹,

Clifford²,

et al. 2009

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The aim of this case-control study of 617 children was to investigate early childhood caries (ECC) risk indicators in a non-fluoridated region in Australia. ECC cases were recruited from childcare facilities, public hospitals and private specialist clinics to source children from different socioeconomic backgrounds. Non-ECC controls were recruited from the same childcare facilities. A multinomial logistic modelling approach was used for statistical analysis. The results showed that a large percentage of children tested positive for Streptococcus mutans if their mothers also tested positive. A common risk indicator found in ECC children from childcare facilities and public hospitals was visible plaque (OR 4.1, 95% CI 1.0–15.9, and OR 8.7, 95% CI 2.3–32.9, respectively). Compared to ECC-free controls, the risk indicators specific to childcare cases were enamel hypoplasia (OR 4.2, 95% CI 1.0–18.3), difficulty in cleaning child’s teeth (OR 6.6, 95% CI 2.2–19.8), presence of S. mutans (OR 4.8, 95% CI 0.7–32.6), sweetened drinks (OR 4.0, 95% CI 1.2–13.6) and maternal anxiety (OR 5.1, 95% CI 1.1–25.0). Risk indicators specific to public hospital cases were S. mutans presence in child (OR 7.7, 95% CI 1.3–44.6) or mother (OR 8.1, 95% CI 0.9–72.4), ethnicity (OR 5.6, 95% CI 1.4–22.1), and access of mother to pension or health care card (OR 20.5, 95% CI 3.5–119.9). By contrast, a history of chronic ear infections was found to be protective for ECC in childcare children (OR 0.28, 95% CI 0.09–0.82). The biological, socioeconomic and maternal risk indicators demonstrated in the present study can be employed in models of ECC that can be usefully applied for future longitudinal studies.

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“…The method uses monoclonal antibodies specific for S. mutans , and the reaction between the antigen and antibodies can be evaluated after just 15 min. The assay has been analysed in only a few studies ; however, those have shown that the method is useful for determining the number of salivary S. mutans .…”

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confidence: 99%

Comparison of Saliva‐Check Mutans and Saliva‐Check IgA Mutans with the Cariogram for caries risk assessment

Wennerholm

Emilson

2013

European J Oral Sciences

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The purpose of the study was to evaluate the combined use of two rapid, semiquantitative detection kits (Saliva-Check Mutans and Saliva-Check IgA Mutans) to assess caries risk using the Cariogram computer-based program as a reference method. Stimulated saliva samples were collected from 71 subjects, 20-73 yr of age, and the number of Streptococcus mutans per ml of saliva was measured using Saliva-Check Mutans. After 15 min, a red line in the test detection window indicated >5 × 10(5) colony-forming units (CFU) of S. mutans per ml of saliva. The concentration of salivary IgA in relation to the number of mutans streptococci was examined using the Saliva-Check IgA Mutans test kit. The presence or absence of a red line after 15 min indicated a high or a low concentration of IgA, respectively. By combining the results obtained from these two test systems, each patient was classified into one of four groups of low to high caries risk. Compared with the Cariogram data, the combined results of both detection kits showed that 39 (95%) of 41 subjects had a high chance of avoiding caries and 20 (67%) of 30 subjects had a low chance. The data suggest that the combination of Saliva-Check Mutans and Saliva-Check IgA Mutans could be used for caries risk assessment.

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A Rapid and Quantitative Detection System for <i>Streptococcus mutans</i> in Saliva Using Monoclonal Antibodies

Cited by 19 publications

References 18 publications

Evaluation of a Japanese “Prevention of Long‐term Care” project for the improvement in oral function in the high‐risk elderly

Evaluation of a Japanese “Prevention of Long‐term Care” project for the improvement in oral function in the high‐risk elderly

Case-Control Study of Early Childhood Caries in Australia

Comparison of Saliva‐Check Mutans and Saliva‐Check IgA Mutans with the Cariogram for caries risk assessment

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