A rapid and selective method for monitoring the growth of coliforms in milk using the combination of amperometric sensor and reducing of methylene blue

Lee, Yuan Geuy; Wu, Hung-Yi; Hsu, Chuan Liang; Liang, Hong; Yuan, Chiun‐Jye; Jang, Hung Der

doi:10.1016/j.snb.2009.06.028

Cited by 20 publications

(10 citation statements)

References 30 publications

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“…The methylene blue reduction test is such an example; however, known flaws of this test include time-consuming and redundant procedures, as well as an inability to discriminate between bacterial types [19]. Lee et al (2009) sought to improve upon the methylene blue reduction method while maintaining its advantages by supplementing it with an amperometric sensor. An amperometric sensor, composed of a circuit with a potentiostat and a pair of electrodes, measures current change [20].…”

Section: Electrical Methods For the Detection Of Bacteriamentioning

confidence: 99%

“…Lee et al (2009) sought to improve upon the methylene blue reduction method while maintaining its advantages by supplementing it with an amperometric sensor. An amperometric sensor, composed of a circuit with a potentiostat and a pair of electrodes, measures current change [20]. Amperometric sensors are small and inexpensive and have been tested in a variety of media to detect changes in bacteria such as E. coli.…”

Section: Electrical Methods For the Detection Of Bacteriamentioning

confidence: 99%

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Milk Spoilage: Methods and Practices of Detecting Milk Quality

Lu¹,

Shiau²,

Wong³

et al. 2013

FNS

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Milk spoilage is an indefinite term and difficult to measure with accuracy. This uncertainty can cause suffering for both milk manufacturers and consumers. Consumers who have been misled by ambiguous expiration dates on milk cartons waste resources by disposing of unspoiled milk or experience discomfort from drinking spoiled milk. Consumers are often unwilling to purchase products close to their inaccurate expiration dates. This consumer behavior has a negative financial impact on milk producers. Inaccurate milk spoilage detection methods also force milk producers to use overly conservative expiration dates in an effort to avoid the legal and economic consequences of consumers experiencing illness from drinking spoiled milk. Over the last decade, new methods have been researched with the purpose of developing more accurate and efficient means of detecting milk spoilage. These methods include indicators based on pH bacteria counts and gas-sensor arrays. This article explores various methods of spoilage detection designed to prevent such consequences. The respective level of effectiveness of each method is discussed, as well as several further approaches to contain freshness regardless of detection

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Section: Electrical Methods For the Detection Of Bacteriamentioning

confidence: 99%

Section: Electrical Methods For the Detection Of Bacteriamentioning

confidence: 99%

Milk Spoilage: Methods and Practices of Detecting Milk Quality

Lu¹,

Shiau²,

Wong³

et al. 2013

FNS

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“…MB can be used for the assessment of activity of live and devitalized yeast cells [13], as well as for the quantitative analysis of antibiotics [14]. The monitoring of the bacterial growth in milk has been reported based on MB reduction [15], and the atomization of the procedure has been proposed [16]. The application of MB for the evaluation of activated sludge DHA is simple, fast and does not require any expensive equipment [17].…”

Section: Introductionmentioning

confidence: 99%

Express-Method for Determination of the Oxidizing Capacity of Activated Sludge and Its Biofilms in Pulp and Paper Mill

Чухчин

Varakin

Rudakova

et al. 2021

Water

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Microbial dehydrogenase activity can help to determine the oxidizing capacity of activated sludge. Here we propose an innovative and automated express-method based on rapid determination of dehydrogenase activity. The measurement is based on the rate of methylene blue reduction by living microbial cells in suspension. A single analysis takes 10 min. The method was adapted for biofilms immobilized on the floating carriers of industrial bioreactors and the kinetics of biological oxidation by activated sludge and biofilms was compared. New parameters were proposed to characterize the biological oxidation under low oxygen levels. The obtained make it possible to quickly determine the dehydrogenase activity of activated sludge and biofilms and promptly monitor the effectiveness of industrial biological wastewater treatment.

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“…Further, implementation of good hygienic and manufacturing practices is far more demanding in developing and tropical countries like India where milk supply sectors remain largely unorganized with less process automation thereby increasing chances of postprocessing contamination. Total coliforms ( Enterobacterales with expression of β‐galactosidase) and Escherichia coli are invariably used as indicators of sanitary conditions in dairy production, processing, and packaging environments which may indirectly correlate with possible presence of enteropathogenic/toxigenic serotypes (da Silva et al, 2001; Lee et al, 2009). E. coli is a common contaminant of raw and processed milk and is an accurate indicator of fecal contamination (Kumar & Prasad, 2010).…”

Section: Introductionmentioning

confidence: 99%

Rapid and miniaturized method for detection of hygiene indicators, Escherichia coli and coliforms, in dairy products

Kadyan

Kumar

Lawaniya

et al. 2020

Journal of Food Safety

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Current investigation was aimed to develop colorimetric tests for rapid detection of Escherichia coli/coliforms. These test (s) for E. coli and coliforms were developed using the modified E. coli selective medium (M-ECSM) and coliform selective medium, respectively. The selective media contain a combination of group specific marker enzymes and selective agents. The marker enzymes were screened using chromogenic substrates wherein β-D-glucuronidase and glutamate decarboxylase were found specific for E. coli while β-D-galactosidase for coliforms. The selectivity of the media was achieved using different concentrations of ampicillin and gentamicin. The optimized test procedures enabled sensitive detection of 0.35 ± 0.10 log cfu/ml of E. coli and 0.57 ± 0.15 log cfu/ml of coliforms at 37 C within 14.30 ± 0.45 and 12.15 ± 0.30 hr, respectively. M-ECSM selectively inhibited major Enterobacteriaceae contaminants (Salmonella, Shigella, and Yersinia) up to 6 log cfu/ml. Moreover, better selectivity of M-ECSM was reported against tested commercial chromogenic media. Field evaluation of developed test (s) reported prevalence of E. coli/coliforms as 57.29/88.54% in 96 raw milk and 16.28/51.16% in 43 pasteurized milk samples. Further, test components were vacuum dried in the form of miniaturized point-of-need test for field application in dairy farms and industries with minimal infrastructural requirements.

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A rapid and selective method for monitoring the growth of coliforms in milk using the combination of amperometric sensor and reducing of methylene blue

Cited by 20 publications

References 30 publications

Milk Spoilage: Methods and Practices of Detecting Milk Quality

Milk Spoilage: Methods and Practices of Detecting Milk Quality

Express-Method for Determination of the Oxidizing Capacity of Activated Sludge and Its Biofilms in Pulp and Paper Mill

Rapid and miniaturized method for detection of hygiene indicators, Escherichia coli and coliforms, in dairy products

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