A rapid and selective solid-phase UV spectrophotometric method for determination of ascorbic acid in pharmaceutical preparations and urine

Ruiz-Medina, A.; Córdova, M.L. Fernández de; Dı́az, A Molina

doi:10.1016/s0731-7085(99)00028-x

Cited by 33 publications

(12 citation statements)

References 20 publications

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“…The AA content in urine corresponds with its content in the serum, and is a valuable marker for the non-invasive analysis [16]. …”

Section: Resultsmentioning

confidence: 99%

“…Methods for AA determination include redox titration with 2,6-dichlorophenolindophenol (DCPIP) [2], HPLC [3], electrochemical [4, 5], fluorescent and spectrophotometric methods [6–16]. …”

Section: Introductionmentioning

confidence: 99%

“…The direct spectrophotometric determination of AA by its absorption in the ultraviolet region is very difficult because of many interfering substances, usually present in the AA containing samples [16]. This problem is overcome by the use of reagents which have specific color reactions with AA.…”

Section: Introductionmentioning

confidence: 99%

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Silica-titania xerogel doped with Mo,P-heteropoly compounds for solid phase spectrophotometric determination of ascorbic acid in fruit juices, pharmaceuticals, and synthetic urine

Morosanova

Моросанова

2017

Chemistry Central Journal

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BackgroundAscorbic acid is one of the most important vitamins to monitor in dietary sources (juices and vitamins) and biological liquids.ResultsSilica and silica-titania xerogels doped with Mo,P-heteropoly compounds (HPC) have been synthesized varying titanium(IV) and HPC content in sol. Their surface area and porosity have been studied with nitrogen adsorption and scanning electron microscopy, their elemental composition has been studied with energy-dispersive X-ray analysis. The redox properties of the sensor material with sufficient porosity and maximal HPC content have been studied with potentiometry and solid phase spectrophotometry and it has been used for solid phase spectrophotometric determination of ascorbic acid. The proposed method is characterized by good selectivity, simple probe pretreatment and broad analytical range (2–200 mg/L, LOD 0.7 mg/L) and has been applied to the analysis of fruit juices, vitamin tablets, and synthetic urine.ConclusionsNew sensor material has been used for simple and selective solid phase spectrophotometric procedure of ascorbic acid determination in fruit juices, vitamin tablets, and synthetic urine.Graphical abstractWe synthesized several silica-titania xerogels doped with Mo,P-heteropoly compounds, studied their properties, and designed the sensor material for solid phase spectrophotometric determination of ascorbic acid in fruit juices, pharmaceuticals, and synthetic urine.

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“…The AA content in urine corresponds with its content in the serum, and is a valuable marker for the non-invasive analysis [16]. …”

Section: Resultsmentioning

confidence: 99%

“…Methods for AA determination include redox titration with 2,6-dichlorophenolindophenol (DCPIP) [2], HPLC [3], electrochemical [4, 5], fluorescent and spectrophotometric methods [6–16]. …”

Section: Introductionmentioning

confidence: 99%

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Silica-titania xerogel doped with Mo,P-heteropoly compounds for solid phase spectrophotometric determination of ascorbic acid in fruit juices, pharmaceuticals, and synthetic urine

Morosanova

Моросанова

2017

Chemistry Central Journal

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“…Solid-phase ultraviolet spectrophotometry was used to determine ascorbic acid concentration in the urine, according to the method of Medina et al 14 Briefly, urine samples were deproteinized with an equivalent volume of 0.6 M trichloroacetic acid solution and filtered through a 0.45 µm membrane filter. Then 1 ml of a buffer solution, pH 8.0, was added to 1 ml of the filtrate, and the volume brought to 10 ml with redistilled water.…”

Section: Determination Of Ascorbic Acid In Urinementioning

confidence: 99%

Ascorbic Acid Enhances Hypoxic Ventilatory Reactivity in Elderly Subjects

Pokorski

Marczak

2003

J Int Med Res

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The reducing properties of ascorbic acid in the carotid body make it a likely modifier of hypoxia-sensing mechanisms. This open-label study aimed to determine the effect of ascorbic acid on the hypoxic ventilatory response (HVR) in a population of elderly women, in whom both hypoxic reactivity and ascorbic acid levels may be deficient. We examined the HVR to progressive eucapnic hypoxia in 18 healthy females aged 60 -80 years, before and after 10 days' ascorbic acid supplementation, given as a sustained release preparation of 1 g twice daily. Respiratory variables were recorded breath by breath, and hypoxic sensitivity was assessed from the linear slopes of minute ventilation and mouth occlusion pressure plotted against oxygen saturation. We found that ascorbic acid increased the HVR by a mean of 44%, this effect being driven by a higher occlusion pressure. We conclude that augmentation of hypoxic reactivity by ascorbic acid may have therapeutic potential in pathologies associated with hypoxia, which frequently develop in old age.

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“…Numerous methods based on flow injection analysis [4][5][6][7][8][9][10][11] , spectrophotometry [12][13][14] , chromatography [15][16][17] and others are available for the determination of ascorbic acid in different matrices. However, when a rapid determination is needed 18,19 , procedures involving modified electrodes are more convenient [20][21] .…”

Section: Introductionmentioning

confidence: 99%

Screen-Printed Electrode Modified With Silver Hexacy Anoferrate-Nafion® for Ascorbic Acid Determination

Mattos

Padilla

Zagal

et al. 2011

J. Chil. Chem. Soc.

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A flow injection system using a new and/or re-used graphite screen-printed electrode modified with silver hexacyanoferrate and a Nafion ® polymer layer (AgHCF/GSPE) was employed for the determination of ascorbic acid in orange juice and drugs samples. Both modified electrodes showed an initial sensitivity of 0.015 A.cm . The response of the electrodes was stable, with no variation of base line after 6-8 hours of continuous operation. With this system it is possible to measure 65-70 samples (20µL) per hour. By using 1.06 mg of AgNO 3 , 4.12 mg of K 3 Fe(CN) 6 , 50.5 mg of KNO 3 and 800µL of HNO 3 , it is possible to obtain between 25 and 30 modified electrodes. The proposed system showed to be inexpensive, versatile, robust and suitable for industrial application. The surface morphology of the bare and/or modified graphite screen-printed electrode was characterized by scanning electron microscopy.

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A rapid and selective solid-phase UV spectrophotometric method for determination of ascorbic acid in pharmaceutical preparations and urine

Cited by 33 publications

References 20 publications

Silica-titania xerogel doped with Mo,P-heteropoly compounds for solid phase spectrophotometric determination of ascorbic acid in fruit juices, pharmaceuticals, and synthetic urine

Silica-titania xerogel doped with Mo,P-heteropoly compounds for solid phase spectrophotometric determination of ascorbic acid in fruit juices, pharmaceuticals, and synthetic urine

Ascorbic Acid Enhances Hypoxic Ventilatory Reactivity in Elderly Subjects

Screen-Printed Electrode Modified With Silver Hexacy Anoferrate-Nafion® for Ascorbic Acid Determination

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