A rapid and sensitive chemiluminescence enzyme-linked immunosorbent assay for the determination of fumonisin B1 in food samples

Qian, Ying; Zhang, Yan; Wang, Shuo; Lee, Nanju Alice; Kennedy, Ivan R.

doi:10.1016/j.aca.2006.07.063

Cited by 72 publications

(55 citation statements)

References 28 publications

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“…The RLU max /IC 50 ratio has been used to estimate the effect of a certain factor on the CLEIA performance. Besides, weak acidic media were better than alkaline media for the CLEIA system, which also agreed with the results of Quan et al 24 Based on these results, the optimum parameters were 20% acetonitrile, 0.5 mol L À1 Na + , and pH 7.4 for CLEIA. For solvent optimization, acetonitrile was selected to increase the solubility of DON.…”

Section: Optimization Of Cleiasupporting

confidence: 90%

A sensitive chemiluminescence enzyme immunoassay for the determination of deoxynivalenol in wheat samples

Zhang

Zhou

2015

Anal. Methods

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A competitive indirect chemiluminescence enzyme immunoassay (ci-CLEIA), capable of rapidly quantifying trace levels of deoxynivalenol (DON), was developed with a monoclonal antibody of DON. In addition, the monoclonal antibody was selected by comparing the half-maximal inhibition concentration (IC 50 ), the limit of detection (LOD, IC 10 ), and the affinity constant with another two antibodies. The effects of organic solvent (acetonitrile), Na + (NaCl) and pH were investigated. Under optimal conditions, the CLEIA allowed DON determination in a linear working range (IC 20 -IC 80 ) of 1.7-170 mg L À1 with an IC 50 value of 17 mg L À1 and a LOD value of 0.49 mg L À1 . Recovery of 90.59-93.16% for the CLEIA was obtained from spiked samples. Furthermore, the results of CLEIA for the authentic samples correlated well with those of gas chromatography. The developed CLEIA would be ideally suitable for screening of DON contamination in wheat samples.

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Section: Optimization Of Cleiasupporting

confidence: 90%

A sensitive chemiluminescence enzyme immunoassay for the determination of deoxynivalenol in wheat samples

Zhang

Zhou

2015

Anal. Methods

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“…HRP derivatives were applied for labeling, and the detection limit of 2,4-dichlorophenoxyacetic acid is 2 pg/ml in a competitive detection method. Wang and coworkers also applied this method for detecting Fumonisin B1 (FB1) in food samples [42]. Roda and coworkers proposed a system for probing Cry1Ab protein in a maize sample employing chemiluminescence and sandwich-type ELISA method [43].…”

Section: Chemiluminescent Labelingmentioning

confidence: 99%

Labeling Oligonucleotides toward the Biomedical Probe

Lee

Kim

2011

Medicinal Chemistry of Nucleic Acids

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“…These instrumental detecting and screening methodologies are generally improper for real-time detection in actual fields and incompatible with demands of food industries which manage large quantity of raw materials and products in a short time. Recently, several ELISA techniques using monoclonal and polyclonal antibodies (mAb and pAb), and automated equipments have been developed and applied for easy and rapid detection of FMs in foods and feeds [5,6]. However, a high detection limit (200 ppb) of a commercial ELISA kit (Neogen Co., Lansing, MI, USA) would limit its use for a broad range of food products.…”

Section: Introductionmentioning

confidence: 99%

Production and characterization of monoclonal antibody and its recombinant single chain variable fragment specific for a food-born mycotoxin, fumonisin B1

Min

Cho

Park

et al. 2009

Bioprocess Biosyst Eng

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Fumonisin B(1) (FMB(1)) is a food-born mycotoxin produced by Fusarium moniliforme. Monoclonal antibody against FMB(1) (anti-FMB(1) mAb) was produced in the hybridoma DV9, which was established from a BALB/c mouse immunized with bovine serum albumin conjugated FMB(1) (FMB(1)-BSA). A competitive direct enzyme-linked immunosorbent assay (ELISA) showed that anti-FMB(1) mAb has about 10 ppb of minimum FMB(1) detection concentration and 220 ppb of 50% inhibition concentration (IC(50)). Much lower cross-reactivity of anti-FMB(1) mAb on ochratoxin A, aflatoxin B(1) and deoxynivalenol provided that anti-FMB(1) mAb was specific for FMB(1). The gene coding single chain variable fragment against FMB(1) (anti-FMB(1) scFv) was cloned from the hybridoma DV9 and was expressed in recombinant Escherichia coli. Insoluble anti-FMB(1) scFv required optimization of its refolding condition, and hence functional scFv was obtained. By using indirect ELISA, about 12-fold lower binding activity of anti-FMB(1) scFv on FMB(1)-BSA was obtained in comparison with that of the parental mAb.

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A rapid and sensitive chemiluminescence enzyme-linked immunosorbent assay for the determination of fumonisin B1 in food samples

Cited by 72 publications

References 28 publications

A sensitive chemiluminescence enzyme immunoassay for the determination of deoxynivalenol in wheat samples

A sensitive chemiluminescence enzyme immunoassay for the determination of deoxynivalenol in wheat samples

Labeling Oligonucleotides toward the Biomedical Probe

Production and characterization of monoclonal antibody and its recombinant single chain variable fragment specific for a food-born mycotoxin, fumonisin B1

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