A rapid and sensitive fluoroimmunoassay based on quantum dot for the detection of chlorpyrifos residue in drinking water

Abstract

A rapid and sensitive indirect competitive fluorescence-linked immunosorbent assay (cFLISA) method based on quantum dots as the fluorescence label coupled with secondary antibody (Ab(2)) for the detection of chlorpyrifos in drinking water has been developed. The cFLISA method allowed for chlorpyrifos determination in a liner working range of 15.2-205.5 ng mL(-1). The 50 % inhibition value (IC(50)) and the limit of detection (LOD) of the cFLISA were 50.2 ng mL(-1) and 8.4 ng mL(-1), while the IC(50) and the LOD of the conventional enzyme linked immunosorbent assay (ELISA) were 95.3 ng- mL(-1) and 16.2 ng mL(-1), respectively. When the concentrations of chlorpyrifos were 200, 100 and 50 ng mL(-1), the recoveries ranged from 90.8 % to 108.2 % with a coefficient of variation (CV) of 7.5 %-15.2 %. In water sample analysis, the results of cFLISA were similar to those obtained from a cELISA and a high performance liquid chromatography (HPLC) method, while the detection time by cFLISA was reduced 0.5 h compared with ELISA. It showed that cFLISA could be used as a new screening method for the detection of pesticide residue.

Determination of Carbendazim and Chlorpyrifos in Selected Fruits and Vegetables Samples Using QuEChERS-HPLC-FD

“…There was a permissible occurrence of carbendazim and chlorpyrifos residues in parsley which grown without application of any pesticide. It confirms that the pesticide residues (carbendazim and chlorpyrifos) were transfered by a surface water to soil agricultural of parsley [8,14,36]. Crum et al [37] had found in his study that the highest sorption of nine common pesticides (include chlopyrifos and carbendazim) to aquatic macrophytes (its structure is very similar to parsley) was chlorpyrifos.…”

“…Chlorpyrifos, has been widely employed in agriculture to kill pests which has a wide environmental endocrine disruptor and its residual mainly exists in crops, livestock, poultry products and also led to their migration into aquifers which is potentially hazardous to health [1,2,5,8,15]. Unfortunately, Chlorpyrifos with a thiophosphoryl (P=S) functional group is important causes of morbidity and mortality following intentional self-poisoning or in cases of occupational or environmental exposure [1,5,16,17].…”

“…The toxic chemicals taken up by plants during cultivation or contaminated during preservation are passed on the food chain causing serious health effect in human beings [3,7]. In recent years, issues of pesticide residue in the environment, vegetables and fruits are arousing more and more public attention [2,8,9]. Most of recently studies find that the pesticide has a direct related with carcinogenicity and neurotoxicity on human health [9,10,11].…”

Simple and sensitive detection of triazophos pesticide by using quantum dots nanobeads based on immunoassay

“…In this reaction, the carboxyl group on the surface of the quantum dot is first activated by N-Hydroxysuccinimide (NHS), and the activated carboxyl group forms an amide bond with the original amino group on the surface of the antibody under the action of EDC (Ji et al, 2005;Wang et al, 2017;Wu et al, 2019). In addition, there are non-covalent bond binding methods, such as biotin and avidin non-covalent bond coupling, covalent bond bonding and amino group and sulfhydryl covalent bond bonding (Chen et al, 2010;Taton, 2000;Zajac, Song, Qian, & Zhukov, 2007).…”

Mechanism to Detect Pesticide Residues in Tealeaves Based on CdZnSe/ZnS Ternary Alloy Quantum Dots

“…Management of pests in tea plantations has largely depended on the use of conventional, neuron toxic, broad-spectrum synthetic chemical pesticides [1]. Organophosphorus (OP) and carbamates (CM) compounds are widely used in agriculture because of the efficiency for insect killing, easy synthesis, and low cost [2]. The above pesticide residues are harmful to the environment, human health, and animals through the nervous system poisoning by the way of inhibiting the activity of AChE, the most important enzyme involved in nerve transmission [3].…”