A rapid and sensitive microscale HPLC method for the determination of indomethacin in plasma of premature neonates with patent ductus arteriousus

Za’abi, Mohammed Al; Dehghanzadeh, Gholamreza; Norris, Ross; Charles, Bruce G.

doi:10.1016/j.jchromb.2005.11.025

Cited by 22 publications

(7 citation statements)

References 13 publications

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“…As a proof of principle, we applied the MnO 2 ‐Gr/GCE to detect indomethacin in two commercial drug samples and compared the results obtained from applying MnO 2 ‐Gr/GCE with a reference procedure using HPLC . Table shows that the two methods agree with each other.…”

Section: Resultsmentioning

confidence: 92%

“…Many analytical methods, such as spectrophotometry, fluorimetry, chemiluminescence (CL), micellar electrokinetic chromatography (MEKC), high performance liquid chromatography (HPLC), LC‐MS and enzyme‐linked immunosorbent assay (ELISA), are used to detect indomethacin. Although these methods successfully detect indomethacin in pharmaceutical and biological samples, some of them lack sensitivity and involve tedious procedure (time consuming or expensive).…”

Section: Introductionmentioning

confidence: 99%

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Manganese dioxide‐graphene nanocomposite film modified electrode as a sensitive voltammetric sensor of indomethacin detection

Zhang

Huang

et al. 2016

Bulletin Korean Chem Soc

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Excess amount of analgesic and anti‐inflammatory drug, such as indomethacin, often leads to serious gastrointestinal complications; therefore, amount of such active compound should be regulated in commercial drugs. This study proposes an efficient analytical technique to detect indomethacin selectively. We prepared and investigated electrochemical properties of a manganese dioxide‐graphene nanocomposite film modified glassy carbon electrode (MnO2‐Gr/GCE). The behavior of the modified electrode as electrocatalyst towards indomethacin oxidation was also examined. The cyclic voltammetric results reveal that the electrocatalytic activity for the oxidation of indomethacin can significantly be enhanced on the MnO2‐Gr/GCE. Indomethacin exhibited a sensitive anodic peak at about 0.90 V at MnO2‐Gr/GCE. The data obtained from differential pulse voltammetry showed that the anodic peak currents were linearly dependent on the indomethacin concentrations in the range of 1.0 × 10−7 to 2.5 × 10−5 mol/L with a detection limit of 3.2 × 10−8 mol/L (S/N = 3). Most importantly, the proposed method shows efficient and selective sensing of indomethacin in commercial pharmaceutical formulations. This is the first report of a voltammetric sensor for indomethacin using MnO2‐Gr/GCE. We believe that this new method can be commercialized for routine applications in laboratories.

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Section: Resultsmentioning

confidence: 92%

Section: Introductionmentioning

confidence: 99%

Manganese dioxide‐graphene nanocomposite film modified electrode as a sensitive voltammetric sensor of indomethacin detection

Zhang

Huang

et al. 2016

Bulletin Korean Chem Soc

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“…The concentration of indomethacin in plasma was determined by HPLC [32] as follows: 10 μL of internal standard solution (200 mg·L –1 naproxen sodium solution) was added into 150 μL of plasma and mixed for 5 min. Then, 15 μL of a phosphate buffer (pH 7.0) and 20 mg of NaCl were added.…”

Section: Methodsmentioning

confidence: 99%

Development of a New Ex Vivo Lipolysis-Absorption Model for Nanoemulsions

Xiao

Liu

2019

Pharmaceutics

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The use of lipid-based formulations (LBFs) in improving the absorption of poorly water-soluble drugs has now well established. Because the in vivo evaluation of LBFs is labor-intensive, in vitro or ex vivo approaches could provide advantages. In this study, a new ex vivo lipolysis-absorption model (evLAM) composed of an intestinal digestion system and an intestinal tissue system was developed to evaluate and predict the in vivo absorption performances of LBFs. Model factors, including the pH of the system and concentrations of d-glucose and pancreatic lipase, were investigated and optimized by a Box-Behnken design. To evaluate this new model, a lipid formulation of indomethacin, which was chosen based on preliminary studies of pseudo-ternary phase diagrams, emulsion droplets, and solubility, was further investigated by an in vivo pharmacokinetic study of rats, the everted gut sac model, and the evLAM, respectively. The absorption percentages obtained from the evLAM were much more similar to the data of rats in vivo than those from the everted gut sac model, showing a preferable in vitro-in vivo correlation (r = 0.9772). Compared with the conventional in vitro and in vivo methods, the evLAM, which allowed precise insights into the in vivo absorption characteristics without much time or a complicated process, could be a better tool for assessing LBFs of poorly water-soluble drugs.

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“…Oral administration of indomethacin may cause severe side effects and it is not the drug of first choice for minor aches and pain [4]. It is extensively bound to plasma proteins and has wide inter‐subject variability in the plasma concentration [5]. Several analytical methods have been reported for the analysis of indomethacin in human biological samples such as liquid chromatography [6, 7], electrochemical detection [8, 9], flow injection analysis [10] and capillary electrophoresis [11, 12].…”

Section: Introductionmentioning

confidence: 99%

Surfactant coated magnetic nanoparticle‐based solid‐phase extraction coupled with spectrophotometric detection for determination of ultra‐trace amounts of indomethacin in biological fluids

Amoli‐Diva

Pourghazi²,

Pourasadollah-Karani

2015

Micro & Nano Letters

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A novel mixed hemimicelle-based solid-phase extraction method using cetyltrimethylammonium bromide (CTAB) adsorbed onto the surface of magnetic Fe 3 O 4 nanoparticles was developed for extraction/preconcentration of ultra-trace amounts of indomethacin from biological fluids. Magnetic nanoparticles were synthesised via a simple chemical co-precipitation method and characterised by a transmission electron microscope, an X-ray diffractometer and Fourier transform infrared techniques. The method utilises the unique properties of Fe 3 O 4 nanoparticles including high surface area and superparamagnetism causing rapid separation (<20 min) and low adsorbent usage (only 50 mg). A comprehensive study on the parameters affecting the adsorption efficiency such as the amount of CTAB, pH of the solution, desorption conditions, amount of Fe 3 O 4 nanoparticles, extraction and preconcentration time, sample volume and ionic strength are presented. Under optimum conditions, the method's linearity was over a range of 25-450 ng ml −1. The limit of detection of 8.6 ng ml −1 , an enrichment factor of 99 and the relative standard deviation (%RSD) of 1.9% (for a concentration of 50 ng ml −1) were obtained. The method was successfully applied to determine indomethacin in human plasma and urine samples. Good recoveries (89-102%) with low % RSD, (1.7-2.7%) were achieved.

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A rapid and sensitive microscale HPLC method for the determination of indomethacin in plasma of premature neonates with patent ductus arteriousus

Cited by 22 publications

References 13 publications

Manganese dioxide‐graphene nanocomposite film modified electrode as a sensitive voltammetric sensor of indomethacin detection

Manganese dioxide‐graphene nanocomposite film modified electrode as a sensitive voltammetric sensor of indomethacin detection

Development of a New Ex Vivo Lipolysis-Absorption Model for Nanoemulsions

Surfactant coated magnetic nanoparticle‐based solid‐phase extraction coupled with spectrophotometric detection for determination of ultra‐trace amounts of indomethacin in biological fluids

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