A rapid and simple HPLC method for the analysis of propofol in biological fluids

Cussonneau, Xavier; Smet, Els De; Lantsoght, Kristof; Salvi, Jean-Paul; Bolon-Larger, Magali; Boulieu, Roselyne

doi:10.1016/j.jpba.2006.10.020

Cited by 36 publications

(26 citation statements)

References 16 publications

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“…However, in the present study, statistically insignifi cant difference (P>0.05) in recovery of crude phenolic extract of irradiated and non-irradiated thyme (34.0±0.45 and 31.1±1.90, respectively) was noted. Because we observed the increase of content only fl avonoid aglycons with trend to reduce the content of fl avonoid glycosides (tentatively identifi ed compounds 5,7,11,14,16,19, changes statistically insignifi cant), so it can be assumed that a few glycoside bonds were cleaved as a result of γ-irradiation. But this presumption should be verifi ed in future research.…”

Section: Resultsmentioning

confidence: 86%

“…Luteolin-7-O-glucoside (13), luteolin (26) and apigenin (28) were recognized as compounds form the fi rst subclass ( Figure 2, Table 1). Based on UV-DAD spectra (data not shown), also some non-identifi ed compounds (5,7,11,14,16,19) could be included to fl avones. Because retention times of peaks corresponding these compounds were shorter than t R of luteolin and apigenin, the compounds with not fully identifi ed structure were probably sugar derivatives of fl avones.…”

Section: Resultsmentioning

confidence: 99%

“…The scans of chromatographic eluent passing through the diode array detector were recorded in a wavelength range from 200 to 600 nm [Karamać et al, 2015]. The fl uorescence detector was set at excitation and emission wavelengths of 276 and 310 nm, respectively [Cussonneau et al, 2007].…”

Section: Analysis Of Phenolic Compoundsmentioning

confidence: 99%

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Effects of γ-Irradiation of Wild Thyme (Thymus serpyllum L.) on the Phenolic Compounds Profile of Its Ethanolic Extract

Janiak

Slavova-Kozakova

Kancheva

et al. 2017

Pol. J. Food Nutr. Sci.

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The presented study revealed that there were changes in the phenolic compounds profi le of extract of wild thyme (Thymus serpyllum L.) after γ-irradiation at the dose of 5 kGy. Ethanolic extracts of irradiated and non-irradiated herb were prepared and their compounds were analyzed by RP--HPLC-DAD technique. Between thirty two detected constituents, twelve phenolic compounds classifi ed as hydroxybenzoic and hydroxycinnamic acids derivatives, fl avones and fl avanones were identifi ed. Among them, caffeic acid derivatives and fl avones predominated with the highest content of rosmarinic acid and luteolin-7-O-glucoside, respectively. Additionally, thymol was recognized in the analyzed extracts. γ-Irradiation slightly affected the quantitative profi le of phenolic compounds of a wild thyme ethanolic extract. Only four constituents differed signifi cantly (P<0.05) in terms of their content in the irradiated and non-irradiated samples. The content of phenolic acids (p-coumaric and caffeic acids) decreased and that of fl avonoid aglycons (luteolin and eriodictyol) increased after the γ-ray treatment.

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Section: Resultsmentioning

confidence: 86%

Section: Resultsmentioning

confidence: 99%

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Effects of γ-Irradiation of Wild Thyme (Thymus serpyllum L.) on the Phenolic Compounds Profile of Its Ethanolic Extract

Janiak

Slavova-Kozakova

Kancheva

et al. 2017

Pol. J. Food Nutr. Sci.

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“…The measurement of propofolblood concentration was performed according to previously described methods [16] . For the measurement of propofol, acetnitrile and internal standardwere added to a plasma sample and vortexed for 1 min.…”

Section: Blood Concentrations Of Propofolmentioning

confidence: 99%

Effectiveness of outpatient percutaneous endoscopic gastrostomy replacement using esophagogastroduodenoscopy and propofol sedation

Horiuchi

Nakayama²,

Kajiyama³

et al. 2012

WJGE

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AIM:To evaluate the effectiveness of outpatient percutaneous endoscopic gastrostomy (PEG) replacement using esophagogastroduodenoscopy (EGD) and propofol sedation. METHODS:We retrospectively assessed the outcome and complications of consecutive patients referred for PEG replacement which was performed using EGD under propofol sedation in the outpatient setting. The success rate, the mean dose of propofol, procedure time, EGD findings, discharge time from endoscopy unit, respiratory depression, and complications within 72 h of the procedure were evaluated. In a subset of these patients, the blood concentrations of propofol were measured. RESULTS:All 221 patients underwent successful PEG replacement. The mean dose of propofol was 34 mg (range, 20-60 mg) with a mean procedure time of 5.9 min (range, 3-8 min). Reflux esophagitis (12 patients), gastric ulcer (5), gastric neoplasm (2), and duodenal ulcer (1) were newly diagnosed at replacement. Discharge from endoscopy unit was possible in 100% of patients 45 min after the procedure. Only 3.6% (8) required transient supplemental oxygen. No complications occurred within 72 h of the procedure. During EGD the level of sedation and propofol blood concentrations after administration of propofol (30 mg) in these PEG patients corresponded to those of propofol (60 mg) in middle aged subjects (control).CONCLUSION: PEG replacement using EGD and propofol sedationin the outpatient setting was safe and practical.

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“…For quantitation of whole blood, an initial cell lysis by freezing [5] or solvent treatment [6] is suggested to efficiently account for the erythrocytebound proportion of propofol. Liquid-liquid (LLE [7,8]) or solid phase extraction (SPE [9,10]) and protein precipitation [5,11] represent the most frequent sample preparation protocols. The drawback of these extraction procedures consists in the necessity of evaporating organic solvents, which may be critical when dealing with volatile analytes.…”

Section: Introductionmentioning

confidence: 99%

Formation of the N-methylpyridinium ether derivative of propofol to improve sensitivity, specificity and reproducibility of its detection in blood by liquid chromatography–mass spectrometry

Thieme¹,

Sachs

Schelling

et al. 2009

Journal of Chromatography B

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A rapid and simple HPLC method for the analysis of propofol in biological fluids

Cited by 36 publications

References 16 publications

Effects of γ-Irradiation of Wild Thyme (Thymus serpyllum L.) on the Phenolic Compounds Profile of Its Ethanolic Extract

Effects of γ-Irradiation of Wild Thyme (Thymus serpyllum L.) on the Phenolic Compounds Profile of Its Ethanolic Extract

Effectiveness of outpatient percutaneous endoscopic gastrostomy replacement using esophagogastroduodenoscopy and propofol sedation

Formation of the N-methylpyridinium ether derivative of propofol to improve sensitivity, specificity and reproducibility of its detection in blood by liquid chromatography–mass spectrometry

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