A Rapid and Simple Method for in vitro Plant Regeneration from Split Embryo Axes of Six Cultivars of Cotton

Hazra, Sulekha; Kulkarni, Anuradha; Banerjee, Anjan K.; Dhage, A. B.; Agrawal, Dinesh Chandra; Krishnamurthy, K. V.; Nalawade, S. M.

doi:10.1023/a:1015129730531

Cited by 8 publications

(2 citation statements)

References 12 publications

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“…Figures 3(d) , 3(e) , and 3(f) show the induction of multiple shoots and rapid shoot elongation from the incised shoot buds in 3510 1C medium. The similar observations on induction and elongation of shoots have been reported in micropropagation studies such as cashew [ 34 ], eucalyptus [ 35 ], lilly [ 36 ], cotton [ 37 ], and yam [ 38 ]. Roots were also produced from the two to three months old plantlet of CCY and CCR cultured in the 3510 1C medium without transferring in the rooting medium ( Figure 4(b) ).…”

Section: Resultssupporting

confidence: 82%

Micropropagation of an Exotic Ornamental Plant,Calathea crotalifera, for Production of High Quality Plantlets

Rozali

Rashid

Taha

2014

The Scientific World Journal

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A successful protocol was established for micropropagation in two selected varieties of exotic ornamental plants, Calathea crotalifera. The effects of different sterilization techniques, explant type, and the combination and concentration of plant growth regulators on shoots induction were studied. The axillary shoot buds explants sprouted from rhizomes in soil free conditions showed high induction rate of shoots with lowest contamination percentage when treated with combination of 30% (v/v) NaOCl, 70% (v/v) ethanol, and 0.3% (w/v) HgCl2. In the present study, the highest number of multiple shoots was obtained in MS basal medium supplemented with 3.5 mg/L 6-Benzylaminopurine (BAP), 1.0 mg/L 1-Naphthaleneacetic acid (NAA), 3% sucrose, and 6 g/L plant agar for both varieties and was used as multiplication medium. Microshoots were highly induced when the young shoot bud explants were incised longitudinally prior subculture. Chlorophyll analysis was studied to test the effects of activated charcoal and L-glutamine on reduction of necrosis problem. The maximum roots induction was recorded on MS medium supplemented with 1.0 mg/L 1-Naphthaleneacetic acid (NAA) compared to indolebutyric acid (IBA). The complete regenerated plantlets were successfully acclimatized in the soilless medium under greenhouse condition. This is the first report of rapid mass propagation for C. crotalifera.

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Section: Resultssupporting

confidence: 82%

Micropropagation of an Exotic Ornamental Plant,Calathea crotalifera, for Production of High Quality Plantlets

Rozali

Rashid

Taha

2014

The Scientific World Journal

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“…Activated charcoal has also been used successfully with some species, acting as an inhibitor of ethylene action. Concentrations between 0.1 and 2% (w/v) of activated charcoal have been described as beneficial for many species (Arena & Pastur 2001;Hazra et al 2002;Fráguas 2003), promoting the adsorption of exudates released by the explant, which cause toxic effects on it. In addition to adsorbing exudates, activated charcoal has the property of reducing the availability of auxin in the culture medium, indirectly reducing the synthesis of ethylene (George 1993).…”

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confidence: 99%

Influence of flask sealing and activated charcoal on the morphogenesis and leaf anatomy of Annona glabra (Annonaceae) cultured in vitro

Santana¹,

Paiva²,

Oliveira³

et al. 2011

Sitientibus sér. Ci. Biol.

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This study evaluated the effects of the type of tube closure and the use of activated charcoal in the morphogenesis and leaf anatomy of Annona glabra (Annonaceae). Nodal segments of A. glabra were inoculated in WPM medium supplemented with 30 g l-1 sucrose and five concentrations of activated charcoal (0, 1, 2, 3 and 4 g l-1). During in vitro culture, nodal segments were kept in test tubes closed in one of three ways: plastic cap sealed with PVC wrap, plastic cap without a PVC wrap and cotton plug. The results showed that the shoots obtained in tubes closed with a plastic cap without a PVC wrap or sealed with a cotton plug had increased growth compared to those obtained in tubes sealed with a plastic cap and a PVC wrap. The presence of activated charcoal caused a reduction of leaf abscission and influenced the anatomical development of the shoots in the tubes sealed with a cotton plug and sealed with a plastic cap without a PVC wrap. The highest means for leaf tissue thickness were obtained from the shoots kept in closed tubes with a plastic lid and a PVC wrap in the absence of activated charcoal.

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The role of activated charcoal in plant tissue culture

Thomas¹

2008

Biotechnology Advances

389

220

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A Rapid and Simple Method for in vitro Plant Regeneration from Split Embryo Axes of Six Cultivars of Cotton

Cited by 8 publications

References 12 publications

Micropropagation of an Exotic Ornamental Plant,Calathea crotalifera, for Production of High Quality Plantlets

Micropropagation of an Exotic Ornamental Plant,Calathea crotalifera, for Production of High Quality Plantlets

Influence of flask sealing and activated charcoal on the morphogenesis and leaf anatomy of Annona glabra (Annonaceae) cultured in vitro

The role of activated charcoal in plant tissue culture

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