A RAPID AND SIMPLE ONE STEP METHOD FOR ISOLATION OF POLY(A)<sup>+</sup>RNA FROM CELLS IN MONOLAYER

Hartmann, K.; Papa, Vincenzo; Brown, Eric J.; Doerries, Ullrich; Rosenthal, Stephen M.; Goldfine, Ira D.

doi:10.1210/endo-127-4-2038

Cited by 46 publications

(22 citation statements)

References 2 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Poly(A) + RNA was isolated as previously described (Hartmann et al, 1990). Samples of RNA (20 mg) or poly(A) + RNA (5mg) were separated by electrophoresis and blotted on Gene Screen Plus nylon membrane (NEN 1, Research Products, Boston, MA, USA) using standard conditions.…”

Section: Rna Isolation and Northern Blot Analysismentioning

confidence: 99%

FGF-8b increases angiogenic capacity and tumor growth of androgen-regulated S115 breast cancer cells

et al. 2001

View full text Add to dashboard Cite

Fibroblast growth factor 8 (FGF-8) is a secreted heparinbinding protein, which has transforming potential. Alternative splicing of the mouse Fgf-8 gene potentially codes for eight protein isoforms (a ± h) which di er in their transforming capacity in transfected cells. S115 mouse mammary tumor cells express a transformed phenotype and secrete FGF-8 in an androgen-dependent manner. In order to study the role of FGF-8 isoforms in the induction of transformed phenotype of breast cancer cells, we over-expressed FGF-8 isoforms a, b and e in S115 cells. Over-expression of FGF-8b, but not FGF-8a or FGF-8e, induced androgen and anchorage independent growth of S115 cells. FGF-8b-transfected S115 cells formed rapidly growing tumors with increased vascularization when injected s.c. into nude mice. FGF-8a also slightly increased tumor growth and probably tumor vascularization but FGF-8e was not found to have any e ects. The angiogenic activity of FGF-8b and heparinbinding growth factor fraction (HBGF) of S115 cell conditioned media was tested in in vitro and in vivo models for angiogenesis using immortomouse brain capillary endothelial cells (IBEC) and chorion allantoic membrane (CAM) assays. Recombinant FGF-8b protein was able to stimulate proliferation, migration, and vessellike tube formation of IBECs. In addition, stimulatory e ect of S115-HBGF on IBE cell proliferation was evident. A positive angiogenic response to FGF-8b was also seen in CAM assay. The results demonstrate that the expression of Fgf-8b is able to promote vessel formation. Angiogenic capacity probably markedly contributes to the ability of FGF-8b to increase tumor growth of androgen-regulated S115 mouse breast cancer cells. Oncogene (2001) 20, 2791 ± 2804.

show abstract

Section: Rna Isolation and Northern Blot Analysismentioning

confidence: 99%

FGF-8b increases angiogenic capacity and tumor growth of androgen-regulated S115 breast cancer cells

et al. 2001

View full text Add to dashboard Cite

show abstract

“…Myogenin cDNA was labeled using random primers to a specific activity of 10 9 c.p.m./µg. Nitrocellulose membranes were pre-hybridized, hybridized and washed as described (Hartmann et al 1990). Autoradiography was carried out, and mRNA abundance was determined by scanning densitometry.…”

Section: Mrna Analysismentioning

confidence: 99%

Functional inactivation of the IGF-I receptor delays differentiation of skeletal muscle cells

Cheng

Adi²,

Wu³

et al. 2000

Journal of Endocrinology

View full text Add to dashboard Cite

Skeletal myoblasts are inherently programmed to leave the cell cycle and begin the differentiation process following removal of exogenous growth factors. Serum withdrawal results in a marked induction of IGF production which is essential for skeletal muscle differentiation in vitro. However, the potential role of the tyrosine kinase IGF-I receptor (thought to be the principal mediator of both IGF-I and II signaling in skeletal muscle) in the decision of myoblasts to begin differentiation following serum withdrawal is unknown. To explore the role of the IGF-I receptor in this decision by skeletal myoblasts, we functionally inactivated endogenous IGF

show abstract

“…Site-specific oligonucleotides (1779-1824 and 1936-1979 of mouse AChE cDNA) (24) were synthesized and 3' endlabeled. Poly(A)+ mRNA was prepared according to the procedure described by Hartmann et al (25). Six micrograms ofpoly(A)+ mRNA was subjected to electrophoresis on a 1% agarose/0.66 M formaldehyde (20 mM Mops/5 mM sodium acetate/i mM EDTA, pH 7.0) gel and transferred to a nylon membrane.…”

mentioning

confidence: 99%

Overexpression of the beta 1 thyroid receptor induces differentiation in neuro-2a cells.

Lebel

Dussault

Puymirat

1994

Proc. Natl. Acad. Sci. U.S.A.

View full text Add to dashboard Cite

To determine the functions of the al and P1thyroid hormone receptors (TRs) Thyroid hormone (3,5,3'-triiodothyronine; T3) is essential for the normal development of the central nervous system. It is generally accepted that T3 produces its effects through interactions with chromatin-associated receptors (1). Thyroid hormone receptors (TR) are encoded by two genes, a and ( c-erbA (2, 3). In rat, these genes give rise to three functional TRs: al, (31, and (32 (3-6). Alternate splicing of the gene is responsible for the formation oftwo 2.6-kb mRNAs that code for two proteins (designated a2 variants) that do not bind T3 (refs. 7

show abstract

A RAPID AND SIMPLE ONE STEP METHOD FOR ISOLATION OF POLY(A)⁺RNA FROM CELLS IN MONOLAYER

Cited by 46 publications

References 2 publications

FGF-8b increases angiogenic capacity and tumor growth of androgen-regulated S115 breast cancer cells

FGF-8b increases angiogenic capacity and tumor growth of androgen-regulated S115 breast cancer cells

Functional inactivation of the IGF-I receptor delays differentiation of skeletal muscle cells

Overexpression of the beta 1 thyroid receptor induces differentiation in neuro-2a cells.

Contact Info

Product

Resources

About

A RAPID AND SIMPLE ONE STEP METHOD FOR ISOLATION OF POLY(A)+RNA FROM CELLS IN MONOLAYER

Cited by 46 publications

References 2 publications

FGF-8b increases angiogenic capacity and tumor growth of androgen-regulated S115 breast cancer cells

FGF-8b increases angiogenic capacity and tumor growth of androgen-regulated S115 breast cancer cells

Functional inactivation of the IGF-I receptor delays differentiation of skeletal muscle cells

Overexpression of the beta 1 thyroid receptor induces differentiation in neuro-2a cells.

Contact Info

Product

Resources

About

A RAPID AND SIMPLE ONE STEP METHOD FOR ISOLATION OF POLY(A)⁺RNA FROM CELLS IN MONOLAYER