A rapid assessment of the radiative properties from a suspension of Chromochloris zofingiensis

Ibañez, Manuel Vicente; Leonardi, R.; Heinrich, Josué Miguel; Steingroewer, Juliane; Walther, Thomas; Krujatz, Felix

doi:10.1016/j.jpap.2020.100007

Cited by 3 publications

(15 citation statements)

References 25 publications

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“…The optical properties corresponding to each sample (

) were evaluated following the methodology developed and described by Ibañez et al [ 20 ]. The simulation algorithm was built on the MC stochastic method.…”

Section: Methodsmentioning

confidence: 99%

“…The ad hoc simulation algorithm was written and executed in MATLAB (2020a). The computational flow diagram, including the decision nodes of the stochastic algorithm, can be found in Ibañez et al [ 20 ]. The solver employed in all the regressions was lsqcurvefit + multistart .…”

Section: Methodsmentioning

confidence: 99%

“…Given the temperatures involved in microalgal cultures, light could be assimilated as a gas of photons which move in different directions with the speed of light. Based on this physical model, Monte Carlo algorithms were developed and computationally implemented with different purposes: one for the determination of the optical properties (OPs) of the suspensions of photosynthetic microorganisms (the spectral absorption (

) and scattering (

) coefficients, and the scattering phase function (

)) [ 19 , 20 ] and the other for the simulation of the REF in PBRs [ 8 , 21 , 22 ]. As a result, it was possible to predict the value of the local volumetric absorption rate of photons (

) and its change with the microalgal culture growth, including the changing degree of stratification of light in the reactor [ 8 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

“…Any TPBR can be understood by treating the three major components of the system separately: the light (yellow), the geometry of the system and operating conditions (orange) and every aspect concerning the biology of the cultured microorganism (green). Adapted and reprinted from [ 20 ] with permission.…”

Section: Introductionmentioning

confidence: 99%

“…Recently, we presented and validated a methodical perspective about the reproduction of outdoor lighting conditions at the laboratory scale and how this information may be employed for scaling up PBRs [ 10 , 24 ]. Later on, we published and validated a methodology for calculating the optical properties necessary to address the light distribution inside a TPBR for changing illuminating conditions through the cultivation process of an eukaryotic microalga [ 20 ]. Here, we sought to utilise this information to construct a predictive tool to estimate the autotrophic bioprocess productivity in a TPBR of 100 litres for a commercially well-known cyanobacterial strain, considering the geometry of the system, the nature of the lighting system in quantity and quality and the physiological state of the cyanobacterial cells measured as the radiative properties of the autotrophic suspension.…”

Section: Introductionmentioning

confidence: 99%

See 4 more Smart Citations

The Assessment of the Real-Time Radiative Properties and Productivity of Limnospira platensis in Tubular Photobioreactors

Ibañez

Leonardi

Krujatz

et al. 2022

Life

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The development of tools to predict the photobioreactors’ (PBRs) productivity is a significant concern in biotechnology. To this end, it is required to know the light availability inside the cultivation unit and combine this information with a suitable kinetic expression that links the distribution of radiant energy with the cell growth rate. In a previous study, we presented and validated a methodology for assessing the radiative properties necessary to address the light distribution inside a PBR for varying illuminating conditions through the cultivation process of a phototrophic microorganism. Here, we sought to utilise this information to construct a predictive tool to estimate the productivity of an autotrophic bioprocess carried out in a 100 [L] tubular photobioreactor (TPBR). Firstly, the time-dependent optical properties over ten batch cultures of L. platensis were calculated. Secondly, the local volumetric rate of photon absorption was assessed based on a physical model of the interaction of the radiant energy with the suspended biomass, together with a Monte Carlo simulation algorithm. Lastly, a kinetic expression valid for low illumination conditions has been utilised to reproduce all the cultures’ experimentally obtained dry weight biomass concentration values. Taken together, time-dependent radiative properties and the kinetic model produced a valuable tool for the study and scaling up of TPBRs.

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“…The optical properties corresponding to each sample (

) were evaluated following the methodology developed and described by Ibañez et al [ 20 ]. The simulation algorithm was built on the MC stochastic method.…”

Section: Methodsmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

) and scattering (

) coefficients, and the scattering phase function (

)) [ 19 , 20 ] and the other for the simulation of the REF in PBRs [ 8 , 21 , 22 ]. As a result, it was possible to predict the value of the local volumetric absorption rate of photons (

) and its change with the microalgal culture growth, including the changing degree of stratification of light in the reactor [ 8 , 23 ].…”

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

See 3 more Smart Citations

The Assessment of the Real-Time Radiative Properties and Productivity of Limnospira platensis in Tubular Photobioreactors

Ibañez

Leonardi

Krujatz

et al. 2022

Life

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Características radiativas de la microalga Coelastrella striolata, productora de carotenoides durante diferentes etapas de cultivo

Martínez Niño

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Optimized Protocol for Microalgae DNA Staining with SYTO9/SYBR Green I, Based on Flow Cytometry and RSM Methodology: Experimental Design, Impacts and Validation

Ihadjadene

Walther

Krujatz

2022

MPs

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Multiple fluorochromes are extensively used to investigate different microalgal aspects, such as viability and physiology. Some of them can be used to stain nucleic acids (DNA). Well-known examples are SYBR Green I and SYTO 9, the latter of which offers several advantages, especially when combined with flow cytometry (FCM)—a powerful method for studying microalgal population heterogeneity and analyzing their cell cycles. However, the effects of these dyes on the microalgae cell physiology have not been fully elucidated yet. A statistical experimental design, using response surface methodology (RSM) with FCM was applied in this study to optimize the DNA staining of a non-conventional microalgae, Chromochloris zofingiensis, with SYBR Green I and SYTO 9, and to optimize the variables affecting staining efficiency, i.e., the dye concentration, incubation time and staining temperature. We found that none of these factors affects the staining efficiency, which was not less than 99.65%. However, for both dyes, the dye concentration was shown to be the most significant factor causing cell damage (p-values: 0.0003; <0.0001) for SYBR Green I and SYTO 9, respectively. The staining temperature was only significant for SYTO 9 (p-value: 0.0082), and no significant effect was observed regarding the incubation time for both dyes. The values of the optimized parameters (0.5 µM, 05 min and 25 °C) for SYTO 9 and (0.5 X, 5 min and 25 °C) for SYBR Green I resulted in the maximum staining efficiency (99.8%; 99.6%), and the minimum damaging effects (12.86%; 13.75%) for SYTO 9 and SYBR Green I, respectively. These results offer new perspectives for improving the use of DNA staining fluorochromes and provides insights into their possible side effects on microalgae.

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A rapid assessment of the radiative properties from a suspension of Chromochloris zofingiensis

Cited by 3 publications

References 25 publications

The Assessment of the Real-Time Radiative Properties and Productivity of Limnospira platensis in Tubular Photobioreactors

The Assessment of the Real-Time Radiative Properties and Productivity of Limnospira platensis in Tubular Photobioreactors

Características radiativas de la microalga Coelastrella striolata, productora de carotenoides durante diferentes etapas de cultivo

Optimized Protocol for Microalgae DNA Staining with SYTO9/SYBR Green I, Based on Flow Cytometry and RSM Methodology: Experimental Design, Impacts and Validation

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