A rapid capillary electrophoresis with electrochemiluminescence method for the assay of human urinary proline and hydroxyproline

Abstract

A novel simultaneous determination method for free and total proline (Pro) and hydroxyproline (Hyp) in human urine was developed, based on capillary electrophoresis (CE) with electrochemiluminescence (ECL) detection, using tris-(2,2'-bipyridyl) ruthenium(II). Experimental conditions, such as the Ru(bpy)(3)2+ concentration, detection potentials, buffer concentration and pH in CE or in the ECL cell, injection voltage and time were investigated in detail. Under optimized conditions, the linear range, detection limit and sample recoveries for the method were 0.01-2 mmol/L (correlation coefficient, 0.9970), 4 micromol/L and 96.4-101.2% in human urine, respectively. The results show that the method has potential applications in monitoring the level of Pro and Hyp in body fluids from patients with bone disease, tumours or chronic uraemia.

Susceptibility ofEscherichia colito the toxic L-proline analogue L-selenaproline is dependent on two L-cystine transport systems

“…; 26 μ mol l −1 by rapid capillary electrophoresis and electrochemiluminescence, Liang et al . ). It also contains smaller amounts of glycine betaine and proline betaine (Chambers and Kunin ; Lever et al .…”

Chemiluminescence detection with separation techniques for bioanalytical applications

“…The ECL detection cell was used for detection of sulpiride in human plasma and urine [68], tramadol and lidocaine in human urine [69], procyclidine in human urine [70], diphenhydramine in rabbit plasma and urine [71], polyamine in human urine [72], reserpine in human urine [73], dioxopromethazine hydrochloride in rat serum and human urine [74], disopyramide in human urine [75,76], ranitidine in human urine [77], aspartate aminotransferase and alanine aminotransferase activities in biofluids [78], and prolidase activity in erythrocytes [79,80]. Other research groups used the same end-column ECL detection cell developed by Wang et al and reported the determination of proline and hydroxyproline in human urine [81], bupivacaine in rat plasma [82], norfloxacin in human urine [83], josamycin in rat plasma [84], pazufloxacin mesylas in human urine [85], and amoxicillin in human urine [86]. Some researchers have also used a flow cell of poly(dimethoxysilane)-Al 2 O 3 for the determination of lidocaine [87] and amino acids and alkaloids [88] in human urine.…”

Kinetic study of paracetamol on prolidase activity in erythrocytes by capillary electrophoresis with Ru(bpy)32+ electrochemiluminescence detection

“…CE-ECL has been successfully applied to the detection of underivatized proline by many groups [16][17][18], and proved to be a relatively simple and sensitive method. This enables CE-ECL as a simple and competent tool to the drug-PLD interaction analysis.…”