A rapid “cusp-covering” to Au nanostar as plasmonic sensor in a single-drop microreactor for the determination of kanamycin in biosamples

Yao, Yao; Tao, Hongbin; Chai, Yaqian; Ju, Jiahe; Zhang, Jinghui; Shen, Wei; Shi, Hang; Liu, Chang; Huang, Cheng; Tang, Sheng

doi:10.1016/j.snb.2022.131993

Cited by 11 publications

(2 citation statements)

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“…Different nanoarchitectures have been developed to enhance the sensitivity of the LSPR biosensors, i.e., to maximize the Δλ upon interaction of biomolecules with nanostructures. These variations encompass a wide array of nanostructures, including spherical nanoparticles, intricate shapes like cubes and stars, core–shell structures, and even pillared mushroom-like configurations. ,− Additionally, these architectures employ an extensive range of materials, including gold, silver, graphene, bimetallic structures, multimetallic compositions, and emerging 2-D materials like MXenes. − Table also shares some of these LSPR substrates, which are combined with microfluidics to form bioassay chips.…”

Section: Introductionmentioning

confidence: 99%

Localized Surface Plasmon Resonance Sensing and its Interplay with Fluidics

Bhalla,

Shen

2024

Langmuir

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In this Feature Article, we discuss the interplay between fluidics and the localized surface plasmon resonance (LSPR) sensing technique, primarily focusing on its applications in the realm of bio/chemical sensing within fluidic environments. Commencing with a foundational overview of LSPR principles from a sensing perspective, we subsequently showcase the development of a streamlined LSPR chip integrated with microfluidic structures. This integration opens the doors to advanced experiments involving fluid dynamics, greatly expanding the scope of LSPR-based research. Our discussions then turn to the practical implementation of LSPR and microfluidics in real-time biosensing, with a specific emphasis on monitoring DNA polymerase activity. Additionally, we illustrate the direct sensing of biological fluids, exemplified by the analysis of urine, while also shedding light on a unique particle assembly process that occurs on LSPR chips. We not only discuss the significance of LSPR sensing but also explore its potential to investigate a plethora of phenomena at liquid−liquid and solid−liquid interfaces. This is particularly noteworthy, as existing transduction methods and sensors fall short in fully comprehending these interfacial phenomena. Concluding our discussion, we present a futuristic perspective that provides insights into potential opportunities emerging at the intersection of fluidics and LSPR sensing.

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Section: Introductionmentioning

confidence: 99%

Localized Surface Plasmon Resonance Sensing and its Interplay with Fluidics

Bhalla,

Shen

2024

Langmuir

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“…Generally, in these procedures, the mass transfer is from an aqueous sample solution to an organic solvent. , However, many biological analytes are polar or hydrophilic, and their direct extraction by organic solvents can be problematic. So typically, to address these problems, derivatization can be undertaken for headspace LPME, − for instance, or other similar procedures (e.g., prevention of dissociation into ions in three-phase (aqueous → organic → aqueous) microextraction) − are applied to permit aqueous–aqueous mass transfer more expeditiously. Still, the analytes suitable for these procedures are limited to those that can be volatilized (after pretreatment) to the headspace or those that can have their ionization (dissociation) initially suppressed and subsequently enabled so as to provide the driving force for them to transfer among three phases.…”

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confidence: 99%

Can Direct-Immersion Aqueous–Aqueous Microextraction Be Achieved When Using a Single-Drop System?

et al. 2022

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For the analysis of biological analytes in complex matrices, it is difficult to achieve extraction of analytes and enrichment in an aqueous−aqueous single-drop microextraction system. In this study, we proposed a pH-dependent polydopamine (PDA)-coated vesicle/Fe 3 O 4 magnetic aqueous−aqueous in a single-drop microreactor (SDMR) for the direct fluorescence detection of glutathione S-transferase (GST), a metabolic enzyme involved with crucial biological processes, in biological samples. After extracting and enriching the GST target from an aqueous−aqueous single-drop interface, the extraction process was conducted rapidly in 6 s in the SDMR system. The GST was first extracted from the sample solution via the GST-Aptamer on the polydopamine-coated vesicle/ Fe 3 O 4 nanospheres (Fe 3 O 4 @PDA@GST-Aptamer). Then, as the pH changed from weakly acidic to weakly alkaline in the SDMR system, the GST and GST-Aptamer were released from Fe 3 O 4 @PDA@GST-Aptamer nanospheres and captured by polydiacetylene vesicles via the capture probe. These changes altered the effective conjugation length and angle of the vesicle trunk, generating a highly enhanced fluorescence signal. This not only achieved the purpose of target enrichment but also reduced interferences posed by matrix effects. The approach can be used for the direct detection of GST in genuine urine and blood without any sample pretreatment. The linear range was 0.005 to 0.5 μg/mL, and the limit of detection was 0.834 ng/mL. The recoveries of GST in genuine blood samples ranged from 90.8 to 108.0% and in urine from 91.6 to 102.8%. The method has the capability of handling complex samples directly by enabling microextraction in an aqueous−aqueous single-drop system.

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