A rapid diagnostic tool for two species of <i><scp>T</scp>etradacus</i> (<scp>D</scp>iptera: <scp>T</scp>ephritidae: <i><scp>B</scp>actrocera</i>) based on species‐specific <scp>PCR</scp>

Fan, Jun; Li, Z. H.; Wu, Jiajiao; Wang, F. X.; Xiong, Hongwei

doi:10.1111/jen.12041

Cited by 18 publications

(16 citation statements)

References 9 publications

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“…The methods of sample preservation, DNA extraction, COI gene amplification (/) and sequencing were reported previously by Jiang et al . (,b). We used Chromas 1.45 (McCarthy ) to review the sequencing results, and bidirectional sequences were assembled for each specimen using the dnaman 5.2.2.0 software (Lynnon Corporation, Quebec, Canada).…”

Section: Methodsmentioning

confidence: 99%

Existence of species complex largely reduced barcoding success for invasive species ofTephritidae: a case study inBactroceraspp.

Fan

Jin

Liang

et al. 2014

Molecular Ecology Resources

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Fruit flies in the family Tephritidae are the economically important pests that have many species complexes. DNA barcoding has gradually been verified as an effective tool for identifying species in a wide range of taxonomic groups, and there are several publications on rapid and accurate identification of fruit flies based on this technique; however, comprehensive analyses of large and new taxa for the effectiveness of DNA barcoding for fruit flies identification have been rare. In this study, we evaluated the COI barcode sequences for the diagnosis of fruit flies using 1426 sequences for 73 species of Bactrocera distributed worldwide. Tree-based [neighbour-joining (NJ)]; distance-based, such as Best Match (BM), Best Close Match (BCM) and Minimum Distance (MD); and character-based methods were used to evaluate the barcoding success rates obtained with maintaining the species complex in the data set, treating a species complex as a single taxon unit, and removing the species complex. Our results indicate that the average divergence between species was 14.04% (0.00-25.16%), whereas within a species this was 0.81% (0.00-9.71%); the existence of species complexes largely reduced the barcoding success for Tephritidae, for example relatively low success rates (74.4% based on BM and BCM and 84.8% based on MD) were obtained when the sequences from species complexes were included in the analysis, whereas significantly higher success rates were achieved if the species complexes were treated as a single taxon or removed from the data set - BM (98.9%), BCM (98.5%) and MD (97.5%), or BM (98.1%), BCM (97.4%) and MD (98.2%).

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Section: Methodsmentioning

confidence: 99%

Existence of species complex largely reduced barcoding success for invasive species ofTephritidae: a case study inBactroceraspp.

Fan

Jin

Liang

et al. 2014

Molecular Ecology Resources

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“…In a previous study, researchers collected the fruit fly samples only in Guizhou, Sichuan and Hunan provinces. Jiang et al only obtained three DNA barcode sequences from B. minax and two DNA barcode sequences from B. tsuneonis [37]. In this case, more reliable species-specific primers were successfully redesigned based on DNA barcode sequences obtained from 963 individuals and download from GenBank with the highest abundance possible, which almost covered all distribution spots of B. minax and B. tsuneonis in China.…”

Section: Species-specific Primer Selection Specificity Test and Sensmentioning

confidence: 99%

“…Asokan et al (2011) designed species-specific primers based on DNA barcode sequences to establish a method for B. dorsalis and B. zonata identification, and this method could reliably identify all life stages of the target species [36]. It is worth noting that in 2014, Jiang et al designed species-specific primers of B. minax and B. tsuneonis based on the DNA barcoding sequences from Guizhou, Sichuan and Hunan Provinces [37]. We found false-positive results when we used these primers for the identification of the two Tetradacus species collected from Guizhou, Sichuan, Yunnan, Hubei, and Chongqing Provinces or city areas, which were not involved in the previous study.…”

mentioning

confidence: 99%

New Species-Specific Primers for Molecular Diagnosis of Bactrocera minax and Bactrocera tsuneonis (Diptera: Tephritidae) in China Based on DNA Barcodes

Zheng

Zhang

Yang

et al. 2019

Insects

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Tephritidae fruit flies (Diptera: Tephritidae) are regarded as important damage-causing species due to their ability to cause great economic losses in fruit and vegetable crops. Bactrocera minax and Bactrocera tsuneonis are two sibling species of the subgenus Tetradacus of Bactrocera that are distributed across a limited area of China, but have caused serious impacts. They share similar morphological characteristics. These characteristics can only be observed in the female adult individuals. The differences between them cannot be observed in preimaginal stages. Thus, it is difficult to distinguish them in preimaginal stages morphologically. In this study, we used molecular diagnostic methods based on cytochrome c oxidase subunit I and species-specific markers to identify these two species and improve upon the false-positive results of previous species-detection primers. DNA barcode sequences were obtained from 900 individuals of B. minax and 63 individuals of B. tsuneonis. Based on these 658 bp DNA barcode sequences of the cytochrome c oxidase subunit I gene, we successfully designed the species-specific primers for B. minax and B. tsuneonis. The size of the B. minax specific fragment was 422 bp and the size of the B. tsuneonis specific fragment was 456 bp. A series of PCR trials ensured the specificity of these two pairs of primers. Sensitivity assay results demonstrated that the detection limit for the DNA template concentration was 0.1~1 ng/µL for these two species. In this study, we established a more reliable, rapid, and low-cost molecular identification method for all life stages of B. minax and B. tsuneonis. Species-specific PCR can be applied in plant quarantine, monitoring and control of B. minax and B. tsuneonis.

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“…; Jiang et al. ). Thus, we were interested in matching DNA barcodes with morphologically identified B .…”

Section: Introductionmentioning

confidence: 98%

“…Previous taxonomic study reported that male B. ocellaris from the Australian region have differences in the ratio of antennal segments compared to males from other zoogeographic regions, even though the genital structure is within the range of intraspecific variation (Menzel et al 2003). In such cases, molecular identification can be helpful for identification of the species complex (Hebert et al 2004a;Smith et al 2007;Burns et al 2008;Rivera and Currie 2009;Naaum et al 2012Naaum et al , 2014Kumar et al 2013;Jiang et al 2014). Thus, we were interested in matching DNA barcodes with morphologically identified B. ocellaris male specimens.…”

Section: Introductionmentioning

confidence: 99%

DNA barcoding of Bradysia (Diptera: Sciaridae) for detection of the immature stages on agricultural crops

Shin

Jung

Heller³

et al. 2014

J Applied Entomology

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We investigated the usefulness of mitochondrial cytochrome c oxidase (COI) DNA barcoding of the genus Bradysia for the detection of immature stages and cryptic species complex. Although the larvae of some species in this genus are agricultural pests, immature stages are rarely identified due to the lack of key morphological characteristics. We constructed partial sequences of the COI gene for 25 species of Bradysia as a first step towards a DNA barcode. Using these data, Bradysia impatiens, B. procera and B. peraffinis were identified from larval specimens collected, respectively, from paprika, ginseng and oak sawdust beds used for cultivating shiitake. Our findings reveal a complex of three species within the B. tilicola group. These species were all identified as important pest B. ocellaris based on the morphology of male genital structures; however, the interspecific genetic divergence of the COI region was significantly greater (16.1–19.4%) than the intraspecific variation in each species. Therefore, B. ocellaris may consist of at least three species. The results demonstrate that COI DNA barcodes are useful for Bradysia species identification.

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A rapid diagnostic tool for two species of Tetradacus (Diptera: Tephritidae: Bactrocera) based on species‐specific PCR

Cited by 18 publications

References 9 publications

Existence of species complex largely reduced barcoding success for invasive species ofTephritidae: a case study inBactroceraspp.

Existence of species complex largely reduced barcoding success for invasive species ofTephritidae: a case study inBactroceraspp.

New Species-Specific Primers for Molecular Diagnosis of Bactrocera minax and Bactrocera tsuneonis (Diptera: Tephritidae) in China Based on DNA Barcodes

DNA barcoding of Bradysia (Diptera: Sciaridae) for detection of the immature stages on agricultural crops

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