1986
DOI: 10.1016/0378-1119(86)90293-3
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A rapid, efficient method for isolating DNA from yeast

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Cited by 342 publications
(169 citation statements)
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“…Transformation of S. cerevisiae and C. albicans was achieved by the lithium acetate method described by Geitz et al (1992) and Braun & Johnson (1997), respectively. Isolation of plasmid DNA from S. cerevisiae was achieved using the procedure described by Holm et al (1986). Plasmid DNA was isolated and purified from E. coli by alkaline lysis (Ish-Horowicz & Burke, 1981) and transformation of E. coli was performed using the calcium chloride method (Mandel & Higa, 1970).…”
Section: Primermentioning
confidence: 99%
“…Transformation of S. cerevisiae and C. albicans was achieved by the lithium acetate method described by Geitz et al (1992) and Braun & Johnson (1997), respectively. Isolation of plasmid DNA from S. cerevisiae was achieved using the procedure described by Holm et al (1986). Plasmid DNA was isolated and purified from E. coli by alkaline lysis (Ish-Horowicz & Burke, 1981) and transformation of E. coli was performed using the calcium chloride method (Mandel & Higa, 1970).…”
Section: Primermentioning
confidence: 99%
“…45% of total DNA. This recovery was based on an estimate of the DNA content of S. cerevisiae made by Holm et al [9].…”
Section: Resultsmentioning
confidence: 99%
“…The procedure for yeast isolation essentially follows that of [9], developed for the rapid isolation of yeast plasmid and chromosomal DNA.…”
Section: Isolation Of Dna From Yeast Cells By An Established Methodsmentioning
confidence: 99%
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“…DNA was prepared by a modification of the technique of Holm et al (1986). Crude nuelei fraetionated as deseribed by Dandekar & Tollervey (1989) were used to prepare chromosomal DNA.…”
Section: (A) Strains and Mediamentioning
confidence: 99%