2013
DOI: 10.1016/j.jneumeth.2013.03.025
|View full text |Cite
|
Sign up to set email alerts
|

A rapid fluorescent method to quantify neuronal loss after experimental intracerebral hemorrhage

Abstract: Neuronal loss in tissue surrounding an intracerebral hemorrhage (ICH) is usually quantified by labor-intensive histological methods that are subject to bias. Fluorescent protein expression has been successfully used as a marker of cell viability in vitro and in retinal studies in vivo, but not in any ICH model to date. The potential of this approach was investigated using transgenic mice that constitutively express the red fluorescent protein variant dTomato in central neurons under the control of the Thy1 pro… Show more

Help me understand this report

Search citation statements

Order By: Relevance

Paper Sections

Select...
4
1

Citation Types

1
27
0

Year Published

2014
2014
2021
2021

Publication Types

Select...
5

Relationship

3
2

Authors

Journals

citations
Cited by 10 publications
(28 citation statements)
references
References 40 publications
1
27
0
Order By: Relevance
“…4), as measured by MTT assay, which correlates well with histological and fluorescent methods (Chen-Roetling et al, 2013). It was increased to 52.6±3.9% by low-dose hemin treatment.…”
Section: Resultsmentioning
confidence: 66%
See 4 more Smart Citations
“…4), as measured by MTT assay, which correlates well with histological and fluorescent methods (Chen-Roetling et al, 2013). It was increased to 52.6±3.9% by low-dose hemin treatment.…”
Section: Resultsmentioning
confidence: 66%
“…The close correlation of this method with stereology-based cell counts of tissue sections has recently been described (Chen-Roetling et al, 2013). At 5 days after striatal blood or collagenase injection, mice (6 – 8/condition) were deeply anesthetized with isoflurane and euthanized by cervical dislocation.…”
Section: Methodsmentioning
confidence: 90%
See 3 more Smart Citations