A Rapid Fluorometric Method for the Determination of Histamine in Canned Tuna

Abstract

A quick, accurate method for the determination of histamine in tuna rish has been developed. Histamine is recovered from fish extracts by ion exchange chromatography, derivatized with o-phthalaldehyde and measured by the intensity of the resulting fluorophore. From filtered crude extracts, 12 samples can be determined per man hour including reconditioning of the ion exchange columns. Recoveries of histamine added at levels of 5 and 30 mg/lOOg to extracts from acceptable quality fish ranged from 98-103%. Recoveries of 94-101% were demonstrated for histamine added at levels of 30 and 100 mg/lOOg to extracts of decomposed fish. Multiple analyses agree within 0.5 mg at a level of 100 mg/lOOg and within 5.0 mg at a level of 100 mg%. The method is at least as accurate as the official AOAC calorimetric procedure and less prone to error by virtue of its simplicity.

Proficiency test scheme for histamine analysis in fishery products in Morocco using the fluorometric method and comparison of results with those obtained with the HPLC reference method

“…This method was used by the LRAR of Casablanca (ONSSA) to compare the PTS results obtained using the fluorometric method [7] and those obtained by the HPLC reference method [5] for the levels 1 and 2 of the PTS samples. …”

“…Lerke and Bell (1976) fluorometric method [7] This method is applied to all fishery products irrespective of their presentation. Histamine is extracted with trichloroacetic acid then fixed on a column filled with an amberlite resin and eluted by hydrochloric acid.…”

“…In spite of the close relationship between biogenic amine content Correspondence: abderrazzak57@gmail.com and sensory change [6], only histamine concentrations in fishery products are regulated for health protection reasons [5]. The objective of our survey was to conduct a Proficiency Test Scheme in order to evaluate the fidelity of histamine analysis in fishery products by the fluorometric method [7]. The official laboratories involved are seeking accreditation according to the ISO/IEC 17025 standard; their participation in the PTS is a requirement of this standard [8].…”

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Abstract.A Proficiency Test Scheme (PTS) for histamine analysis in fishery products using the Lerke and Bell (1976) fluorometric method was conducted in 26 different Moroccan laboratories. The goal of this PTS was to assess the abilities of a network of seven government laboratories and 19 laboratories performing auto-controls on fish processing units in Morocco.

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Histamine formation and microbiological changes in endemic Chalcalburnus tarichi Pallas 1811 (Inci Kefali) stored at 4 °C

“…Histamine was analyzed spectrofluorimetrically following the procedure of Lerke and Bell (1976). The fluorescence of the compound was measured by means of a Luminescence spectrofluorometer (Perkin Elmer LS 50 B Wellesley, USA), using an excitation wavelength of 350 nm and an emission wavelength of 450 nm.…”