A rapid genotyping method for the vivax malaria transmission-blocking vaccine candidates, Pvs25 and Pvs28

Tsuboi, Takafumi; Kaneko, O.; Cao, Yaming; Tachibana, Masashi; Yakushijin, Yoshihiro; Nagao, Takayoshi; Kanbara, Hiroji; Torii, Motomi

doi:10.1016/j.parint.2004.01.012

Cited by 15 publications

(17 citation statements)

References 8 publications

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“…The Pvs25-II haplotype was similar to some isolates from Bangladesh (GenBank: BAA94348.1-BAA94350.1) [25] and Thailand (GenBank: BAC66001.1) [15]. The Pvs25-III haplotype was common with isolates from Iran (GenBank: ACJ54129.1, ACJ54128.1) [24], Thailand (GenBank: BAC66003.1) [15], Indonesia (GenBank: AAV33639.1) [23], India (GenBank: BAA94346.1) [25] and Bangladesh (GenBank: BAA94347.1) [25]. Only Pvs25-IV was unique in China.…”

Section: Resultsmentioning

confidence: 70%

“…The Pvs28-II haplotype was similar with those from Thailand (GenBank: BAC66011.1) [15] and Bangladesh (GenBank: BAA94370.1, BAA94369.1) [25]. Parasite isolates from Yunnan contained some region-specific haplotypes.…”

Section: Resultsmentioning

confidence: 72%

“…The Pvs28-I haplotype in Yunnan isolates was also common in isolates from Iran (GenBank: ACJ54136.1) [24] and Bangladesh (GenBank: BAA94352.1, BAA94353.1, BAA94371.1, BAA94362, BAA94363) [25]. The Pvs28-II haplotype was similar with those from Thailand (GenBank: BAC66011.1) [15] and Bangladesh (GenBank: BAA94370.1, BAA94369.1) [25].…”

Section: Resultsmentioning

confidence: 88%

“…Pvs25 and Pvs28 contained conserved structures, which are characterized by a secretory N-terminal signal sequence followed by four epidermal growth factor (EGF) domains and a glycosylphosphatidylinositol (GPI) anchor [21]. To date, genetic diversity of pvs25 and pvs28 genes has been surveyed in several Asian countries, including South Korea [22], Indonesia [23], Iran [24], India [25,26], Bangladesh [25], and Thailand [15]. In this study, we aim to analyze the genetic diversity of these candidate TBVs genes in parasite isolates from Yunnan Province, China.…”

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of transmission-blocking vaccine candidates Pvs25 and Pvs28 in Plasmodium vivax isolates from Yunnan Province, China

et al. 2011

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BackgroundTransmission-blocking vaccines (TBVs) have been considered an important strategy for disrupting the malaria transmission cycle, especially for Plasmodium vivax malaria, which undergoes gametocytogenesis earlier during infection. Pvs25 and Pvs28 are transmission-blocking vaccine candidates for P. vivax malaria. Assessment of genetic diversity of the vaccine candidates will provide necessary information for predicting the performance of vaccines, which will guide us during the development of malaria vaccines.ResultsWe sequenced the coding regions of pvs25 and pvs28 from 30 P. vivax isolates from Yunnan Province, identifying five amino acid haplotypes of Pvs25 and seven amino acid haplotypes of Pvs28. Among a total of four mutant residues, the predominant haplotype of Pvs25 only had the I130T substitution. For Pvs28, a total of eight amino acid substitutions were identified. The predominant haplotype of Pvs28 had two substitution at positions 52 (M52L) and 140 (T140S) with 5-6 GSGGE/D tandem repeats at the end of fourth EGF-like domain. Most amino acid substitutions were common with previous reports from South Asian isolates. Although the nucleotide diversity of pvs28 (π = 0.0034 ± 0.0012) was significantly higher than pvs25 (π = 0.0013 ± 0.0009), it was still conserved when compared with the blood stage vaccine candidates.ConclusionsGenetic analysis revealed limited genetic diversity of pvs25 and pvs28, suggesting antigenic diversity may not be a particular problem for Sal I based TBVs in most P. vivax-endemic areas of China.

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Section: Resultsmentioning

confidence: 70%

Section: Resultsmentioning

confidence: 72%

Section: Resultsmentioning

confidence: 88%

Section: Introductionmentioning

confidence: 99%

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Genetic diversity of transmission-blocking vaccine candidates Pvs25 and Pvs28 in Plasmodium vivax isolates from Yunnan Province, China

et al. 2011

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“…However, antigens expressed at a parasite sexual stage, such as OSPs, are immunologically concealed from the host immune system, and hence the chance that antigenic variations occur may be low. Indeed, OSPs of P. falciparum and P. vivax were shown to have minimal antigenic variations even in field isolates collected from remote regions of the world (19,27). This is an important characteristic of ideal vaccines.…”

Section: Discussionmentioning

confidence: 99%

Malaria Ookinete Surface Protein-Based Vaccination via the Intranasal Route Completely Blocks Parasite Transmission in both Passive and Active Vaccination Regimens in a Rodent Model of Malaria Infection

et al. 2009

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Malaria vaccines based on ookinete surface proteins (OSPs) of the malaria parasites block oocyst development in feeding mosquitoes and hence disrupt the parasite life cycle and prevent the disease from being transmitted to other individuals. To investigate whether a noninvasive mucosal vaccination regimen effectively blocks parasite transmission in vivo, Plasmodium yoelii Pys25, a homolog of the Pfs25 and Pvs25 OSPs of Plasmodium falciparum and Plasmodium vivax, respectively, was intranasally (i.n.) administered using a complement-deficient DBA/2 mouse malaria infection model, in which a highly elevated level of oocysts develops in feeding mosquitoes. Vaccinated mice developed a robust antibody response when the vaccine antigen was given together with cholera toxin adjuvant. The induced immune serum was passively transferred to DBA/2 mice 3 days after infection with P. yoelii 17XL, and Anopheles stephensi mosquitoes were allowed to feed on the infected mice before or after serum transfusion. This passive immunization completely blocked oocyst development; however, immune serum induced by the antigen or adjuvant alone did not have such a profound antiparasite effect. Further, when i.n. vaccinated mice were infected with the parasite and then mosquitoes were allowed to directly feed on the infected mice, complete blockage of transmission was again observed. To our knowledge, this is the first time that mucosal vaccination has been demonstrated to be efficacious for directly preventing parasite transmission from vaccinated animals to mosquitoes, and the results may provide important insight into rational design of nonparenteral vaccines for use against human malaria.Malaria is one of the most important infectious diseases, and the levels of mortality and morbidity are high, especially among children in developing countries in Africa, Asia, and South America. Implementation of malaria control measures, such as antimalaria drug chemotherapy and insecticide-treated bed nets, has made a significant contribution to reducing the incidence of malaria in many parts of the world. However, these control measures may not be sufficient, and therefore new tools, including vaccines, should be included in a new malaria control campaign for local elimination and final eradiation of malaria from the globe (7). A promising strategy to counteract global malaria endemicity is to develop highly efficacious vaccines, and several promising candidates have been intensively investigated (7,20); vaccines targeting asexual stages (i.e., sporozoite, hepatic, and erythrocytic stages) are designed to prevent infection and reduce disease severity, while vaccines that target the sexual stage, in which the parasite undergoes sporogonic development in anopheline mosquitoes, prevent vector-mediated transmission of the parasite from person to person (4,8,14,17,25). Although transmission-blocking vaccines do not directly prevent infection, they reduce parasite infectivity for the vector and consequently lower the mosquito infection rate and the freque...

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Exploring genetic polymorphisms among Plasmodium vivax isolates from the Thai-Myanmar borders using circumsporozoite protein (pvcsp) and ookinete surface protein (pvs25) encoding genes

Guled,

Na-Bangchang,

Chaijaroenkul

2024

Parasitol Res

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A rapid genotyping method for the vivax malaria transmission-blocking vaccine candidates, Pvs25 and Pvs28

Cited by 15 publications

References 8 publications

Genetic diversity of transmission-blocking vaccine candidates Pvs25 and Pvs28 in Plasmodium vivax isolates from Yunnan Province, China

Genetic diversity of transmission-blocking vaccine candidates Pvs25 and Pvs28 in Plasmodium vivax isolates from Yunnan Province, China

Malaria Ookinete Surface Protein-Based Vaccination via the Intranasal Route Completely Blocks Parasite Transmission in both Passive and Active Vaccination Regimens in a Rodent Model of Malaria Infection

Exploring genetic polymorphisms among Plasmodium vivax isolates from the Thai-Myanmar borders using circumsporozoite protein (pvcsp) and ookinete surface protein (pvs25) encoding genes

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