A rapid high performance liquid chromatographic determination of methyldopa in human serum with fluorescence detection and alumina extraction: Application to a bioequivalence study

Bahrami, Gholamreza; Kiani, Amir; Mirzaeei, Shahla

doi:10.1016/j.jchromb.2005.12.045

Cited by 29 publications

(14 citation statements)

References 11 publications

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“…Various analytical techniques have been reported for the determination of MD in pharmaceutical formulations and biological fluids such as high-performance liquid chromatography [10][11][12], potentiometry [13], spectrophotometry [14][15][16], NMR spectroscopy [17], and fluorometry [18]. Some of these methods are not simple and others are time consuming or involve procedures with rigorous control of the experimental conditions or require expensive equipment and consequently are not suitable for routine analysis.…”

Section: Page 4 Of 40mentioning

confidence: 99%

Application of multivariate optimization to electrochemical determination of methyldopa drug in the presence of diclofenac at a nanostructured electrochemical sensor

Ghoreishi

Saeidinejad

Behpour

et al. 2015

Sensors and Actuators B: Chemical

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Section: Page 4 Of 40mentioning

confidence: 99%

Application of multivariate optimization to electrochemical determination of methyldopa drug in the presence of diclofenac at a nanostructured electrochemical sensor

Ghoreishi

Saeidinejad

Behpour

et al. 2015

Sensors and Actuators B: Chemical

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“…The methyldopa and phenylephrine can be detected in biological solutions using some existing methods, including spectrofluorimetric, chromatographic and electrochemical techniques. High performance liquid chromatography (HPLC) using electrochemical detector, fluorescence or UV detections are able to measure the drug concentration in urine and plasma samples . However, it may be boring and time consuming the sample preparation in most of these approaches.…”

Section: Introductionmentioning

confidence: 99%

Analysis of methyldopa in the presence of phenylephrine using electrocatalytic effect of a ferrocene derivative at a surface of feather like La³⁺/ZnO nano‐flowers modified carbon paste electrode

Pourtaheri

Taher

Beitollahi

et al. 2018

Applied Organom Chemis

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The purpose of the present study was to introduce a newly designed approach for simultaneous determination of methyldopa and phenylephrine using modified carbon paste electrode with feather like La3+/ZnO nano‐flowers and N‐(ferrocenylmethylidene) fluoren‐2‐amine (La3+/ZnONFMF2ACPE). According to the results from the electrochemical experiments, oxidation current of methyldopa on the modified electrode surface was incremented and its oxidation occurred at a potential about 110 mV less positive than that of an unmodified carbon paste electrode. A linear response was observed for the electrode at different methyldopa concentrations (0.2 to 500.0 μM). The existence of phenylephrine did not induce any change in the modified electrode sensitivity to methyldopa, indicating that they could be measured simultaneously or independently. Real human body samples were used to test our technique efficacy in detecting methyldopa and phenylephrine.

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“…Of the techniques used in several procedures most are based on kinetic measurements (James;Hoo;William, 2006), highperformance liquid chromatography (Bahrami, Kiani, Mirzaeei, 2006;Muzzi et al, 2008;Li et al, 2010), voltammetry (Rezaei, Askarpour, Ensafi, 2013), spectrophotometry (Gadkariem et al, 2009;Brasil, 2010;Matos,Silva;Ribeiro, 2012; and diffuse reflectance spectroscopy (Ribeiro, Pezza, Pezza, 2006).…”

Section: Introductionmentioning

confidence: 99%

Development and application of a portable instrument for drugs analysis in pharmaceutical preparations

Ribeiro

Pezza

et al. 2015

Braz. J. Pharm. Sci.

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This article describes the application and performance of an inexpensive, simple and portable device for colorimetric quantitative determination of drugs in pharmaceutical preparations. The sensor is a light detector resistor (LDR) incorporated into a black PTFE cell and coupled to a low-cost multimeter (Ohmmeter). Quantitative studies were performed with captopril/p-chloranil/H 2 O 2 and methyldopa/ ammonium molybdate systems. Calibration curves were obtained by plotting the electrical resistance of the LDR against the concentration of the colored species in the ranges 1.84 × 10 -4 to 1.29 × 10 -3 mol L -1 and 5.04 × 10 -4 to 2.52 × 10 -3 mol L -1 for captopril/p-chloranil/H 2 O 2 and methyldopa/ammonium molybdate systems, respectively, exhibiting good coefficients of determination. Statistical analysis of the results obtained showed no significant difference between the proposed methodologies and the official reported methods, as evidenced by the t-test and variance ratio at a 95% confidence level. The results of this study demonstrate the applicability of the instrument for simple, accurate, precise, fast, in situ and low-cost colorimetric analysis of drugs in pharmaceutical products. Uniterms:Optical sensor/application/colorimetric analysis. Instrumentation/drugs dosage. Drugs/ quantitative analysis. Drugs/dosage. Pharmaceutical formulations/quality control.Este artigo descreve o desenvolvimento e a aplicação de um dispositivo portátil, simples e barato para a determinação colorimétrica quantitativa de fármacos em formulações farmacêuticas. O sensor é um resistor detector de luz (RDL) colocado numa célula de PTFE e acoplado a um multímetro de baixo custo. Os estudos quantitativos foram realizados utilizando captopril/p-cloranil/H 2 O 2 e metildopa/molibdato de amônio como sistemas reacionais. As curvas de calibração foram obtidas através da representação gráfica da resistência elétrica do RDL contra a concentração dos complexos coloridos formados nas faixas de 1,84 × 10 -4 e 1,29 × 10 -3 mol L -1 e 5,04 × 10 -4 e 2,52 × 10 -3 mol L -1 para captopril/p-cloranil/H 2 O 2 e de metildopa/molibdato de amônio, respectivamente, com bons coeficientes de determinação. As análises estatísticas dos resultados obtidos mostraram que não houve diferença significativa entre os métodos propostos e os métodos oficiais como evidente a partir dos testes "t-Student" e F-Fisher, com nível de confiança de 95%. Os resultados deste estudo demonstram que o instrumento proposto neste trabalho é simples, de fácil operação, baixo custo e apresentou boa exatidão e boa precisão para o doseamento de fármacos em medicamentos.Unitermos: Sensor óptico/aplicação/análise colorimétrica. Instrumentação/doseamento de fármacos. Fármacos/análise quantitativa. Fármacos/doseamento. Formulações farmacêuticas/controle de qualidade.

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A rapid high performance liquid chromatographic determination of methyldopa in human serum with fluorescence detection and alumina extraction: Application to a bioequivalence study

Cited by 29 publications

References 11 publications

Application of multivariate optimization to electrochemical determination of methyldopa drug in the presence of diclofenac at a nanostructured electrochemical sensor

Application of multivariate optimization to electrochemical determination of methyldopa drug in the presence of diclofenac at a nanostructured electrochemical sensor

Analysis of methyldopa in the presence of phenylephrine using electrocatalytic effect of a ferrocene derivative at a surface of feather like La³⁺/ZnO nano‐flowers modified carbon paste electrode

Development and application of a portable instrument for drugs analysis in pharmaceutical preparations

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A rapid high performance liquid chromatographic determination of methyldopa in human serum with fluorescence detection and alumina extraction: Application to a bioequivalence study

Cited by 29 publications

References 11 publications

Application of multivariate optimization to electrochemical determination of methyldopa drug in the presence of diclofenac at a nanostructured electrochemical sensor

Application of multivariate optimization to electrochemical determination of methyldopa drug in the presence of diclofenac at a nanostructured electrochemical sensor

Analysis of methyldopa in the presence of phenylephrine using electrocatalytic effect of a ferrocene derivative at a surface of feather like La3+/ZnO nano‐flowers modified carbon paste electrode

Development and application of a portable instrument for drugs analysis in pharmaceutical preparations

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Analysis of methyldopa in the presence of phenylephrine using electrocatalytic effect of a ferrocene derivative at a surface of feather like La³⁺/ZnO nano‐flowers modified carbon paste electrode