A rapid homogenous bioassay for detection of thyroid-stimulating antibodies based on a luminescent cyclic AMP biosensor

Miao, Lynn Yihong; Kim, Hannah J.; Whitlatch, Kindra; Jaiswal, Depesh; Navarro, Adriana; Egan, Richard; Olivo, Paul D.

doi:10.1016/j.jim.2021.113199

Cited by 12 publications

(3 citation statements)

References 26 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…While RIA requires the extraction of AVP from the plasma, our assay is homogeneous and does not require time-consuming procedures 7 , 8 . The assay developed in this study requires time for cell seeding and transfection, but we believe that this problem can be solved using cryopreserved cells, as reported previously 9 , 10 . These findings demonstrate the potential of this simplified assay for the quantification of plasma AVP.…”

Section: Discussionmentioning

confidence: 73%

“…We hypothesized that a biosensor-based cAMP accumulation assay could be an alternative method for measuring blood AVP levels. A recent study reported the successful application of a similar biosensor-based assay to measure levels of thyroid-stimulating autoantibodies (TSAbs) using cells co-expressing the thyroid-stimulating hormone receptor (TSHR) and a cAMP biosensor 9 , 10 . In this assay, the cAMP biosensor measured the increase in intracellular cAMP levels triggered by TSHR activation promoted by blood TSAbs.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

A cAMP-biosensor-based assay for measuring plasma arginine–vasopressin levels

Doi,

Kawakami,

Ikuta

et al. 2024

Sci Rep

View full text Add to dashboard Cite

Arginine–vasopressin (AVP), a cyclic peptide hormone composed of nine amino acids, regulates water reabsorption by increasing intracellular cyclic adenosine monophosphate (cAMP) concentrations via the vasopressin V2 receptor (V2R). Plasma AVP is a valuable biomarker for the diagnosis of central diabetes insipidus (CDI) and is commonly measured using radioimmunoassay (RIA). However, RIA has several drawbacks, including a long hands-on time, complex procedures, and handling of radioisotopes with special equipment and facilities. In this study, we developed a bioassay to measure plasma AVP levels using HEK293 cells expressing an engineered V2R and a cAMP biosensor. To achieve high sensitivity, we screened V2R orthologs from 11 various mammalian species and found that the platypus V2R (pV2R) responded to AVP with approximately six-fold higher sensitivity than that observed by the human V2R. Furthermore, to reduce cross-reactivity with desmopressin (DDAVP), a V2R agonist used for CDI treatment, we introduced a previously described point mutation into pV2R, yielding an approximately 20-fold reduction of responsiveness to DDAVP while maintaining responsiveness to AVP. Finally, a comparison of plasma samples from 12 healthy individuals demonstrated a strong correlation (Pearson's correlation value: 0.90) between our bioassay and RIA. Overall, our assay offers a more rapid and convenient method for quantifying plasma AVP concentrations than existing techniques.

show abstract

Section: Discussionmentioning

confidence: 73%

Section: Introductionmentioning

confidence: 99%

A cAMP-biosensor-based assay for measuring plasma arginine–vasopressin levels

Doi,

Kawakami,

Ikuta

et al. 2024

Sci Rep

View full text Add to dashboard Cite

show abstract

“…In Graves' disease, both inhibitory and excitatory TRAb antibodies have been observed, so that the importance of TRAb antibodies cannot be conclusively assessed [41]. Further investigation will require assays that can differentiate between TRAb subtypes [42,43]. Alternative explanations include the direct effects of cytokines on thyroid function; e.g., interferon alpha and IL-2 were demonstrated to elicit hypothyroidism.…”

Section: Discussionmentioning

confidence: 99%

Primary Thyroid Dysfunction Is Prevalent in Hidradenitis Suppurativa and Marked by a Signature of Hypothyroid Graves’ Disease: A Case–Control Study

Abu Rached,

Dietrich,

Ocker

et al. 2023

JCM

View full text Add to dashboard Cite

Hidradenitis suppurativa (HS) is a chronic skin disease that can have an association with endocrine disorders. There is conflicting information in the literature regarding the role of the thyroid gland in HS. This study aimed to close this knowledge gap and investigate how thyroid disease is involved in patients with HS. We carried out a case–control study with a total of 160 patients, of whom 108 were patients with HS and 52 were controls matched for age and sex. Parametric and non-parametric methods were used to analyze the results. We calculated structural parameters of thyroid homeostasis to detect subclinical thyroid disease, non-thyroid disease syndrome and other forms. The severity of HS was not associated with thyroid hormone levels and antibodies (p > 0.05). HS patients with or without hypothyroidism had decreased FT4 levels and a decreased thyroid secretory capacity (SPINA-GT). Titers of TSH receptor autoantibodies (TRAb) were significantly higher in smoking HS patients compared to non-smokers (median: 1.18 vs. 1.08; p = 0.042). The rate of subclinical hypothyroidism was significantly higher in HS patients; thus, subclinical hypothyroidism is an important comorbidity of HS (p < 0.05). Further studies are needed to investigate whether the chronic inflammation of HS is a cause of increased rates of (subclinical) hypothyroidism.

show abstract

Bioassays for thyrotropin receptor autoantibodies

Olivo

2023

Best Practice & Research Clinical Endocrinology & Metab

View full text Add to dashboard Cite

A rapid homogenous bioassay for detection of thyroid-stimulating antibodies based on a luminescent cyclic AMP biosensor

Cited by 12 publications

References 26 publications

A cAMP-biosensor-based assay for measuring plasma arginine–vasopressin levels

A cAMP-biosensor-based assay for measuring plasma arginine–vasopressin levels

Primary Thyroid Dysfunction Is Prevalent in Hidradenitis Suppurativa and Marked by a Signature of Hypothyroid Graves’ Disease: A Case–Control Study

Bioassays for thyrotropin receptor autoantibodies

Contact Info

Product

Resources

About