A rapid immunoperoxidase assay for the detection of specific IgG antibodies to Chlamydia trachomatis.

Cevenini, Roberto; Rumpianesi, Fabio; Donati, Manuela; Sarov, Israel

doi:10.1136/jcp.36.3.353

Cited by 27 publications

(12 citation statements)

References 18 publications

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“…Other methods of determining the presence of chlamydial antibodies are the whole inclusion immunofluorescent (WIIF) (78,145,146) and the whole inclusion immunoperoxidase (25) (46). In addition, this study confirmed others that have shown TWAR antibodies in a large proportion of the general adult population.…”

Section: Serologic Methodssupporting

confidence: 76%

Laboratory diagnosis of human chlamydial infections

Barnes

1989

Clin Microbiol Rev

167

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Chlamydia trachomatis is a human pathogen that causes ocular disease (trachoma and inclusion conjunctivitis), genital disease (cervicitis, urethritis, salpingitis, and lymphogranuloma venereum), and respiratory disease (infant pneumonitis). Respiratory chlamydioses also occur with infection by avian strains of C. psittaci or infection by the newly described TWAR agent. Diagnosis of most acute C. trachomatis infections relies on detection of the infecting agent by cell culture, fluorescent antibody, immunoassay, cytopathologic, or nucleic acid hybridization methods. Individual non-culture tests for C. trachomatis are less sensitive and specific than the best chlamydial cell culture system but offer the advantages of reduced technology and simple transport of clinical specimens. Currently available nonculture tests for C. trachomatis perform adequately as screening tests in populations in which the prevalence of infection is greater than 10%. A negative culture or nonculture test for C. trachomatis does not, however, exclude infection. The predictive value of a positive nonculture test may be unsatisfactory when populations of low infection prevalence are tested. Tests that detect antibody responses to chlamydial infection have limited utility in diagnosis of acute chlamydial infection because of the high prevalence of persistent antibody in healthy adults and the cross-reactivity due to infection by the highly prevalent C. trachomatis and TWAR agents. Assays for changes in antibody titer to the chlamydial genus antigen are used for the diagnosis of respiratory chlamydioses. A single serum sample that is negative for chlamydial antibody excludes the diagnosis of lymphogranuloma venereum.

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Section: Serologic Methodssupporting

confidence: 76%

Laboratory diagnosis of human chlamydial infections

Barnes

1989

Clin Microbiol Rev

167

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“…The absence of the IgA class of antibody in any of the positive sera also supports the view that none was the result of a recent or current infection (Cevenini et al 1983). It is apparent that neither sheep farmers nor especially their wives have had an undue frequency of antibody-inducing chlamydial infections in comparison with other types of farmer or with non-farming members of the same community.…”

Section: Methodssupporting

confidence: 57%

“…All sera were tested subsequently by a similar IPA technique against inclusions of the ZC26 ovine strain of C. psittaci in McCoy cell cultures by the procedure described for the IPAZYME kits (Cevenini et al 1983). In each batch of tests, the reactivity of the antigen slides was tested by titration with mouse monoclonal antichlamydial antibody by standard immunofluorescence techniques recommended by the manufacturer (Boots Celltech, Slough, England), and against the human sera from patients with psittacosis or genital tract chlamydial infections (described above) using the IPA technique.…”

Section: Methodsmentioning

confidence: 99%

“…It is known (Richmond & Caul, 1975) that infected tissue culture cells containing mature chlamydial inclusions have such broad reactivity. Thus, all sera were first titrated in an immuno-peroxidase assay (IPA) using cells infected with a lymphogranuloma-venereum (LGV) strain of C. trachomatis (Cevenini et al 1983). All sera were further titrated in IPA with a recent ovine strain of C. psittaci (ZC26) as a possible means of differentiating immune responses against the two species of Chiamydia by differences in the incidence or titre of antibodies shown by the two preparations.…”

Section: Introductionmentioning

confidence: 99%

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Chlamydial antibodies in farmers in north-west England

Hobson

Morgan‐Capner²

1988

Epidemiol. Infect.

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SUMMARYBecause of recent reports of abortion in farmers' wives following infection with ovine strains of Chlamydia psittaci during pregnancy, the distribution of chlamydial antibodies was studied in rural populations in north-west England, where endemic chlamydial infection with abortion is common in sheep.Immunoperoxidase assays with C. trachomatis and ovine C. psittaci showed no significant differences in either the frequency or titres of antibodies between sheep farmers and other types of farmer or non-farming adults living in the same areas. The frequency and titres of antibodies in farmers' wives were no greater than in farmers, and were unrelated to their previous obstetric history or type of farming. Overall, 62/255 (24%) of this rural population had antibody detected by C. trachomatis antigen and only 30/255 (13 %) detected by C. psittaci antigen. The possible significance of these findings is discussed. This survey does not suggest that the risk of infection with C. psittaci is especially high in people working with sheep, but the complications following infection during pregnancy deserve the specific instructions that have been given to pregnant women to avoid exposure, especially during lambing, in farming and veterinary work.

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“…Therefore a simple and sensitive serological test could be of considerable clinical and epidemio!ogical value and also decrease the cost of screening. The established serologic tests for the identification of chlamydial antibodies are the c o m p l e m e n t fixation test, indirect inclusion immunofluorescence, the immunoperoxidase assays and the microimmunofluorescence test (28,25,3).…”

Section: Introductionmentioning

confidence: 99%

Diagnosis of Chlamydia trachomatis infection ? culture versus serology

et al. 1988

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The diagnostic value of different laboratory methods in detecting Chlamydia trachomatis infections in high risk groups was analysed. The efficiency of a direct specimen test was compared with serology (IgG and IgM ELISA) and culture in L929 cells, stained either with fluorescein conjugated monoclonal antibodies or with iodine. Patients (no. = 1041) with localized genital infections attending a STD clinic, sexual contacts and patients with ascending infections from urological and gynecological clinics were examined. Chlamydia trachomatis was detected in 225 patients: 210 (93.3%) were reactive in the direct test (smears stained with monoclonal antibodies), whereas culture missed only 5 (sensitivity 97.8%) when stained by the same method. Cultures stained with iodine produced the lowest recovery rate (73.8%), but this rate increased to 80.9% when a second passage was performed. In addition the prevalence of Neisseria gonorrhoeae, Mycoplasma hominis, Ureaplasma urealyticum, Candida albicans and Trichomonas vaginalis was investigated. In patients with non-gonococcal urethritis (no. = 331) and cervicitis (no. = 353), Chlamydia trachomatis was isolated in 32.3% and 12.8% respectively. However, this pathogen could be isolated in only 3 (15.8%) out of 19 patients with epididymitis and 15 (14%) out of 107 patients with adnexitis, although 66.7% and 93.3% respectively had specific IgG antibodies. Specific IgM could by detected with a sandwich ELISA in patients with adnexitis (46.7%), epididymitis (33.3%), cervicitis (22.2%), non-gonococcal urethritis (14%) and in the sexual partners of patients with genital infections (35.7%). The direct specimen test with monoclonal antibodies is the method of choice for the diagnosis of a C. trachomatis infection in patients with urethritis and cervicitis.(ABSTRACT TRUNCATED AT 250 WORDS)

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A rapid immunoperoxidase assay for the detection of specific IgG antibodies to Chlamydia trachomatis.

Cited by 27 publications

References 18 publications

Laboratory diagnosis of human chlamydial infections

Laboratory diagnosis of human chlamydial infections

Chlamydial antibodies in farmers in north-west England

Diagnosis of Chlamydia trachomatis infection ? culture versus serology

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