A rapid in vivo test for chromosomal damage☆

Heddle, John A.

doi:10.1016/0027-5107(73)90035-3

Cited by 705 publications

(228 citation statements)

References 6 publications

Supporting

Mentioning

191

Contrasting

Unclassified

Order By: Relevance

“…1) [1][2][3][4][5][6] . The cytokinesis-block micronucleus (CBMN) assay is the preferred method for measuring MNi in cultured human and/or mammalian cells because scoring is specifically restricted to once-divided BN cells, which are the cells that can express MNi [3][4][5][6] .…”

Section: Introductionmentioning

confidence: 99%

“…The ''cytome'' concept implies that every cell in the system studied is scored cytologically for its viability status (necrosis, apoptosis), its mitotic status (mononucleated, BN, multinucleated) and its chromosomal damage or instability status (presence of MNi, NPBs, NBUDs and number of centromere probe signals among nuclei or MNi of BN cell if such molecular tools are used in combination with the assay) (Figs. [1][2][3][4][5]. For these reasons, it is now appropriate to refer to this technique as the cytokinesisblock MN cytome (CBMN Cyt) assay.…”

Section: Introductionmentioning

confidence: 99%

See 1 more Smart Citation

Cytokinesis-block micronucleus cytome assay

2007

View full text Add to dashboard Cite

Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Cytokinesis-block micronucleus cytome assay

2007

View full text Add to dashboard Cite

“…The occurrence of micronuclei is increased following exposure to clastogenic agents that cause double-strand DNA breaks and also by aneugens that disrupt chromosomal segregation [1][2]. The in vivo rodent micronucleus (MN) test is widely used to identify or otherwise study chemicals with genotoxic potential [3][4][5].…”

Section: Introductionmentioning

confidence: 99%

Reticulocyte and micronucleated reticulocyte responses to gamma irradiation: dose–response and time-course profiles measured by flow cytometry

Dertinger

Tsai

Nowak

et al. 2007

Mutation Research/Genetic Toxicology and Environmental Mutagene

View full text Add to dashboard Cite

A flow cytometric, anti-CD71-based method was used to measure peripheral blood reticulocyte and micronucleated reticulocyte frequencies in response to 137 Cs total body irradiation (TBI). In three independent experiments, groups of five female C57BL/6N mice were irradiated at graded doses up to 3 Gy, and peripheral blood specimens were collected at 43 hrs post-irradiation. Whereas the frequency of reticulocytes declined over the range of doses studied, micronucleated reticulocyte incidence was observed to increase in a dose-dependent manner up to 1 Gy. At doses greater than approximately 1 Gy, micronucleated reticulocyte frequencies declined with increasing exposure. These responses were highly reproducible, with significant effects on reticulocyte and micronucleated reticulocyte frequencies observed for the lowest dose studied (0.125 Gy). A timecourse experiment was performed to test whether radiation-induced cell cycle delay may explain saturation of the micronucleated reticulocyte endpoint at doses > 1 Gy. For this experiment, groups of four female C57BL/6N mice were exposed to 1, 1.5, or 2 Gy TBI, and blood collection occurred at 12 hr intervals from 43 to 115 hrs post-exposure. Reduced reticulocyte frequencies were observed for each dose studied, and the recovery of reticulocytes was increasingly delayed with higher radiation doses. Maximal micronucleated reticulocyte frequencies were observed at 43 or 55 hrs, with progressively lower values at later time points. At no time did micronucleated reticulocyte frequencies induced by 1.5 or 2 Gy significantly exceed that observed for 1 Gy at 43 hrs. These timecourse data suggest that radiation-induced cell cycle delay cannot account for the micronucleated reticulocyte downturn phenomenon observed at doses greater than 1 Gy. An alternate hypothesis is discussed whereby apoptotic elimination of severely damaged bone marrow erythroid precursors plays a dominant role in saturating the radiation-induced micronucleated reticulocyte response observed for C57BL/6N mice.

show abstract

“…Genotoxic effects can result by micronuclei (MN) induction. Micronuclei are cytoplasmic chromatin-containing bodies formed during the metaphase/anaphase transition of mitosis (cell division) either from a whole lagging chromosome (aneugenic event) or lagging acentric chromosome fragments caused by chromosomal breakage (clastogenic event) which do not integrate in the daughter cell nuclei following anaphase (Heddle, 1973;Schmidt, 1975;Miller et al, 1998).…”

mentioning

confidence: 99%

Micronucleus occurrence in diploid and triploid rainbow trout (Oncorhynchus mykiss Walbaum)

Strunjak‐Perović¹,

Čož‐Rakovac²,

Popović³

2003

Vet. Med. - Czech

View full text Add to dashboard Cite

ABSTRACT:The aim of the study was to observe the influence of different ploidy levels in fish on micronucleus occurrence. Twenty minutes a�er fertilization, one group of rainbow trout eggs was exposed to water temperatures of 26°C in duration of 20 minutes to induce triploidy. Second group was kept in water temperature of 10°C, which is optimal for development of rainbow trout. The frequency of micronucleated erythrocytes was determined in the peripheral circulation of rainbow trout 67 days (following absorption of the yolk -swim-up stage) and 128 days (fry stage) post fertilization. There was a significant difference (P < 0.001) between frequency of micronucleated erythrocytes of diploid (1.10 ± 0.96‰) and triploid (2.41 ± 1.28‰) fish at swim-up stage. Increased mean values of micronucleus in diploid (1.80 ± 1.57‰) and triploid (5.92 ± 3.80‰) fry were also recorded.

show abstract

A rapid in vivo test for chromosomal damage☆

Cited by 705 publications

References 6 publications

Cytokinesis-block micronucleus cytome assay

Cytokinesis-block micronucleus cytome assay

Reticulocyte and micronucleated reticulocyte responses to gamma irradiation: dose–response and time-course profiles measured by flow cytometry

Micronucleus occurrence in diploid and triploid rainbow trout (Oncorhynchus mykiss Walbaum)

Contact Info

Product

Resources

About