A Rapid Method for Determination of Resveratrol in Wines by HPLC-MS

Abstract

A new, sensitive, high throughput liquid chromatography coupled with mass spectrometry (LC-MS=MS) method for quantification of trans-andcisresveratrol in wine samples was elaborated. During method development the influence of multiple factors on the ionization efficiency of resveratrol was studied. These parameters included ion source (APCI and ESI, both being operated in positive and negative ionization mode), percentage of aqueous and organic phase, type of organic solvent, and concentration of additives. The optimum conditions were as follows: a mobile phase consisting of a mixture of 1 mM ammonium acetate=acetonitrile (73=27, v=v) with a flow rate of 1 mL=min, MS=MS detection with an APCI interface, operated in negative ionization mode. The monitored ion transition was m=z 227!184.7. Calibration curves were generated for both isomers of resveratrol in the range of 10.47-837.86 ng=mL (trans isomer) and 9.12 À 730.14 ng=mL (cis isomer), respectively. The developed method has been successfully applied to the analysis and comparison of trans-and cis-resveratrol content of 20 different Romanian wine samples.

Antioxidant and antimicrobial effects of grape pomace extracts

“…Chromatographic and mass spectrometric data acquisition were performed using Chemstation software (Agilent Technologies, Palo Alto, CA, USA), version B.01.03 and LC/MSD Trap Control (Bruker Daltonik, GmbH, Bremen, Germany), version 5.3, while data processing was performed using LC/MSD Data Analysis and Quant Analysis software (Bruker Daltonik, GmbH, Bremen, Germany), version 1.7. In order to find the optimum ion source (MS interface) and mobile phase composition the signal to noise (S/N) ratios were calculated for each injection [17].…”

Development and Validation of a HPLC Method Using a Monolithic Column for Quantification of trans-Resveratrol in Lipid Nanoparticles for Intestinal Permeability Studies

“…Considering the methods found in the literature (Table S1) for the quantification of trans-resveratrol in wines, grapes, and plasma using spectrophotometric, 5,12−31 fluorometric, 12,28,32−34 mass spectrometry, 14,20,31,35 or electrochemical detection, 16 high detection limits, within the micromolar per liter range, were generally attained. As our research aim includes the evaluation of in vitro intestinal permeability of trans-resveratrol inside lipid nanoparticles, by using Caco-2 cell monolayers, a detection limit close to nanomolar per liter of resveratrol is required.…”

“…In this context, the objective of this work was the development and validation of a simple, sensitive, and selective high-performance liquid chromatography (HPLC) method, according to the International Conference on Harmonization (ICH) guidelines on bioanalytical method validation, for the quantification of trans -resveratrol incorporated in lipid nanoparticles, comprehending both solid lipid nanoparticles (SLNs) and nanostructured lipid carriers (NLCs). Considering the methods found in the literature (Table S1) for the quantification of trans -resveratrol in wines, grapes, and plasma using spectrophotometric, ,− fluorometric, ,,− mass spectrometry, ,,, or electrochemical detection, high detection limits, within the micromolar per liter range, were generally attained. As our research aim includes the evaluation of in vitro intestinal permeability of trans -resveratrol inside lipid nanoparticles, by using Caco-2 cell monolayers, a detection limit close to nanomolar per liter of resveratrol is required.…”

Rapid, Highly-Sensitive and Ecologically Greener Reversed-Phase/Normal-Phase HPTLC Technique with Univariate Calibration for the Determination of Trans-Resveratrol

“…Standard method performance requirements and validation protocols have also been set by the Association of Official Agricultural Chemists for the determination of TRV in dietary supplements and dietary ingredients [27]. Some liquid chromatography-mass spectrometry (LC-MS) assays were also utilized for TRV analysis in plant materials, wine samples and plasma samples [28][29][30][31]. Different high-performance thin-layer chromatography (HPTLC) techniques have also been utilized for TRV analysis in a variety of samples such as wine samples, plant extracts, pharmaceutical products, herbal products and polyherbal products [3,4,[32][33][34][35].…”