A Rapid Method for the Determination of Soluble and Insoluble Dietary Fiber: Comparison with AOAC Total Dietary Fiber Procedure and Englyst's Method

Abstract

Total dietary fiber (TDF) was measured in 16 foods by three methods: a rapid enzyme-NDF (neutral detergent fiber) procedure supplemented with a separate procedure for soluble (SOL) fiber, the AOAC TDF method and the comprehensive Englyst's procedure. The NDF+ SOL method needed less operator time than the AOAC method and its TDF values were in agreement with the AOAC method (y = 0.98 X -0.39; r = 0.997) and with the Englyst's method (y = 1.17 X -0.19; r = 0.996). The constituent sugars of polysaccharides in NDF+ SOL residues were similar to those by the Englyst's method, indicating an adequate estimation of dietary fiber in foods and food products.

Advances in dietary fibre characterisation. 1. Definition of dietary fibre, physiological relevance, health benefits and analytical aspects

“…Most comparative and collaborative studies have examined the Englyst procedure and/or the enzymic-gravimetric AOAC method for their performance in terms of practical feasibility for routine measurements as well as intra-and inter-laboratory variance (Asp & Johansson, 1981;Cummings et al 1985;Mongeau & Brassard, 1986Schweizer et al 1988;Wood et al 1993;Pendlington et al 1996). These studies have led to the following conclusions:…”

Dietary Fiber Guidelines in the Exchange Lists for Menu Planning: Should they be revised?

“…Two enzymatic-gravimetric procedures are being used for human food analysis: the AOAC method (9) and the procedure developed by Mongeau and Brassard (16) (19,20). Two approaches are emerging as major enzymatic-chemical procedures for fiber analysis: the Englyst method (21), which is a modification of the Southgate procedure (22); and the Theander method (7,8), which has been recently coined the Uppsala method (23).…”

Carbohydrates and Related Food Components: INFOODS Tagnames, Meanings, and Uses

“…Several modifications (Schaller, 1977;Robertson and van Soest, 1977;Mongeau and Brassard, 1986) incorporate an amylase treatment to overcome the problem (AACC insoluble dietary fiber method 32-200, AOAC method 992.6, AACC method 32-06, respectively).…”