A rapid method for the base ratio determination of bacterial DNA

Cashion, P.; Holder-Franklin, M. A.; McCully, J.; Franklin, M.

doi:10.1016/0003-2697(77)90720-5

Cited by 1,005 publications

(799 citation statements)

References 10 publications

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“…DNA from the new strain LBS3 T and A. horikoshii was isolated by chromatography on hydroxyapatite by the procedure of Cashion et al (1977). DNA-DNA hybridization and computation were carried out at the DSMZ using the methods of De Ley et al (1970) and Jahnke (1992), with the modification described (Escara & Hutton, 1980 ;Huss et al, 1983).…”

Section: Methodsmentioning

confidence: 99%

Anaerobranca gottschalkii sp. nov., a novel thermoalkaliphilic bacterium that grows anaerobically at high pH and temperature.

Prowe¹,

Antranikian²

2001

International Journal of Systematic and Evolutionary Microbiology

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A novel thermoalkaliphilic, obligately anaerobic bacterium was isolated from a humid soil sample of a hot inlet of Lake Bogoria, Kenya. The newly isolated strain grows optimally at pH 9 5 and 50-55 SC and its growth range is pH 6 0-10 5 and 30-65 SC. Unlike the already known thermoalkaliphiles, the strain grows heterotrophically on a variety of mono-and polysaccharides (glucose, ribose, mannose, fructose, sucrose, maltose, starch, pullulan, xylan and cellulose) and on proteinaceous substrates such as yeast extract, peptone and tryptone. No dissimilatory sulfate reduction was observed, whereas thiosulfate was found to enhance growth when glucose or starch were used as substrates. Under optimal conditions, the doubling time is 48 min. Sodium ions are necessary for growth, with an optimal concentration of 230 mM (1 % NaCl, w/v) at pH 9 5. The rod-shaped cells are motile in the exponential growth phase under optimal growth conditions. Despite the Gram-negative staining and negative KOH assay, the strain is a Gram-positive organism, having an atypically thin cell wall. A sheath-like structure occurs at the cell separation area and parts of a surface layer-like structure were also observed. Based on physiological properties and molecular biological analysis, the strain falls within the radiation of the clostridia and represents a new species of Anaerobranca within the Clostridium/Bacillus subphylum of the Gram-positive bacteria. Strain LBS3 T (l DSM 13577 T ) is named Anaerobranca gottschalkii sp. nov. and is designated as the type strain.

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Section: Methodsmentioning

confidence: 99%

Anaerobranca gottschalkii sp. nov., a novel thermoalkaliphilic bacterium that grows anaerobically at high pH and temperature.

Prowe¹,

Antranikian²

2001

International Journal of Systematic and Evolutionary Microbiology

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“…Isolation of DNA (Cashion et al, 1977) and determination of the DNA mol YO G + C values by HPLC (Mesbah et al, 1989) followed described procedures.…”

Section: Methodsmentioning

confidence: 99%

Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α-Proteobacteria

Pukall

Frühling

et al. 1999

International Journal of Systematic and Evolutionary Microbiology

126

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Analysis of PCR products of 16s rDNA of 680 isolates from Mediterranean Sea mesocosm experiments with taxon-specif ic 165 rDNA oligonucleotides revealed that 262 isolates belonged to the 01 subclass of the class Proteobacteria. Partial 165 rDNA sequence analysis of selected isolates and oligonucleotide probing with a Sulfitobacter-specif ic 165 rDNA probe affiliated 33 strains to the genus Sulfitobacter. Analysis of the Haelll digest pattern of 165 rDNA revealed the presence of two groups; while 30 strains showed a pattern identical with that obtained for Sulfitobacter pontiacus DSM 10014T, a second group of three strains had a unique pattern that was different from that of the type strain. Five isolates of group 1 and one isolates of group 2, strain CH-B427T, were selected for detailed taxonomic analysis. All six isolates closely resembled the type strain Sulfitobacter pontiacus DSM 10014T in physiological reactions. However, strain CH-B427T differed quantitatively in the composition of fatty acids from Sulfitobacter pontiacus DSM 10014T and showed only 98.2% 16s rDNA sequence similarity with strain DSM 10014T. DNA-DNA reassociation value obtained for strains DSM 10014T and CH-B427T revealed 46 O/ O similarity. Based on the results of DNA-DNA reassociation and discrete differences in the nucleotide composition of 16s rDNA, a new species of the genus Sulfitobacter is proposed, designated Sulfitobacter mediterraneus sp. nov., the type strain being strain CH-B427T (= DSM 122443.

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“…Whole-cell fatty acids were analysed as described by Pirttija$ rvi et al (1996). DNA was isolated by chromatography on hydroxyapatite (Cashion et al, 1977) and the base composition was determined according to Mesbah et al (1989) and Tamaoka & Komagata (1984).…”

Section: S Elo and Othersmentioning

confidence: 99%

Paenibacillus borealis sp. nov., a nitrogen-fixing species isolated from spruce forest humus in Finland.

Elo¹,

Suominen²,

Kämpfer³

et al. 2001

International Journal of Systematic and Evolutionary Microbiology

105

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Seven spore-forming, nitrogen-fixing bacterial isolates from spruce forest humus in Finland were studied using the polyphasic approach. PCR amplification of 16S rRNA gene fragment with specific primers showed that the isolates were members of Paenibacillus. Levels of 16S rDNA similarity between the isolates were 97 3-100 0 % and those between the isolates and other Paenibacillus species were 90 3-96 5 %. The highest similarities were observed with Paenibacillus azotofixans and Paenibacillus durus. Ribotyping with EcoRI and PvuII restriction showed a high diversity in the Paenibacillus species and distinguished the isolates from these closely related species. The main whole-cell fatty acids were anteiso-C 15 :0 (33-48 %), straight-chain C 14 :0 (7-21 %) and C 16 :0 (9-20 %), and iso-C 15 :0 (6-15 %). Electron microscopy revealed a unique striped morphology of the spore surfaces. Based on phylogenetic inference and phenotypic and chemotaxonomic characteristics, these isolates are proposed as a new species, Paenibacillus borealis sp. nov., the type strain of which is KK19 T (l DSM 13188 T l CCUG 43137 T ).

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A rapid method for the base ratio determination of bacterial DNA

Cited by 1,005 publications

References 10 publications

Anaerobranca gottschalkii sp. nov., a novel thermoalkaliphilic bacterium that grows anaerobically at high pH and temperature.

Anaerobranca gottschalkii sp. nov., a novel thermoalkaliphilic bacterium that grows anaerobically at high pH and temperature.

Sulfitobacter mediterraneus sp. nov., a new sulfite-oxidizing member of the α-Proteobacteria

Paenibacillus borealis sp. nov., a nitrogen-fixing species isolated from spruce forest humus in Finland.

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