A rapid method for the determination of mycotoxins in edible vegetable oils by ultra-high performance liquid chromatography-tandem mass spectrometry

Hidalgo-Ruiz, José L.; Romero‐González, Roberto; Vidal, José Luis Martı́nez; Frenich, Antonia Garrido

doi:10.1016/j.foodchem.2019.03.003

Cited by 88 publications

(44 citation statements)

References 22 publications

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“…PSA exhibited a good performance for the vast majority of analytes ( Figure 1b) but was unable to recover other important mycotoxins, such as AFB1 and AFG1. The moderate affinity of PSA with polar compounds may explain low recoveries for aflatoxins, being consistent with other works based on oily matrices [29,30]. Similarly, extraction with C18 was efficient for most compounds and only some low-polarity mycotoxins showed recoveries out of the range set, like ZAN (150%) and β-ZEL (155%).…”

Section: Evaluation Of the Type Of Sorbent For Clean-upsupporting

confidence: 88%

Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole Orbitrap High-Resolution Mass Spectrometry for Multi-Residue Analysis of Mycotoxins and Pesticides in Botanical Nutraceuticals

Narváez

Rodríguez-Carrasco

Castaldo

et al. 2020

Toxins

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Cannabidiol (CBD) food supplements made of Cannabis sativa L. extracts have quickly become popular products due to their health-promoting effects. However, potential contaminants, such as mycotoxins and pesticides, can be coextracted during the manufacturing process and placed into the final product. Accordingly, a novel methodology using ultra-high-performance liquid chromatography coupled with quadrupole Orbitrap high-resolution mass spectrometry (UHPLC-Q-Orbitrap HRMS) was developed to quantify 16 mycotoxins produced by major C. sativa fungi, followed by a post-target screening of 283 pesticides based on a comprehensive spectral library. The validated procedure was applied to ten CBD-based products. Up to six different Fusarium mycotoxins were found in seven samples, the most prevalent being zearalenone (60%) and enniatin B1 (30%), both found at a maximum level of 11.6 ng/g. Co-occurrence was observed in four samples, including one with enniatin B1, enniatin A and enniatin A1. On the other hand, 46 different pesticides were detected after retrospective analysis. Ethoxyquin (50%), piperonyl butoxide (40%), simazine (30%) and cyanazine (30%) were the major residues found. These results highlight the necessity of monitoring contaminants in food supplements in order to ensure a safe consumption, even more considering the increase trend in their use. Furthermore, the developed procedure is proposed as a powerful analytical tool to evaluate the potential mycotoxin profile of these particular products.

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Section: Evaluation Of the Type Of Sorbent For Clean-upsupporting

confidence: 88%

Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole Orbitrap High-Resolution Mass Spectrometry for Multi-Residue Analysis of Mycotoxins and Pesticides in Botanical Nutraceuticals

Narváez

Rodríguez-Carrasco

Castaldo

et al. 2020

Toxins

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“…Sample preparation is of primary importance in mycotoxin determination because it affects every chromatographic determination. The most common solvents used for the extraction of mycotoxins from foodstuff are methanol-water and acetonitrile-water [26,28,30]. In addition, other solvents, such as acetone [31,32], ethyl acetate [33,34] and chloroform [35,36], are also used for mycotoxin extraction.…”

Section: Resultsmentioning

confidence: 99%

“…Different acids were added to modify the pH (HCl 37% and HCOOH 0.1%, CH3COOH 0.1%, or none), methods of stirring (ultrasonic bath or rotary shaker) and incubation times (15,30,45,60 and 90 min) were also tested (data not shown). Extraction conditions can be greatly influenced by matrix composition and a unique method is not always suitable for all types of food matrices.…”

Section: Resultsmentioning

confidence: 99%

HPLC-MS/MS Method for the Detection of Selected Toxic Metabolites Produced by Penicillium spp. in Nuts

Spadaro

Meloni

Siciliano

et al. 2020

Toxins

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Penicillium spp. are emerging as producers of mycotoxins and other toxic metabolites in nuts. A HPLC-MS/MS method was developed to detect 19 metabolites produced by Penicillium spp. on chestnuts, hazelnuts, walnuts and almonds. Two extraction methods were developed, one for chestnuts and one for the other three nuts. The recovery, LOD, LOQ and matrix effect were determined for each analyte and matrix. Correlation coefficients were always >99.99%. In walnuts, a strong signal suppression was observed for most analytes and patulin could not be detected. Six strains: Penicillium bialowiezense, P. brevicompactum, P. crustosum, P. expansum, P. glabrum and P. solitum, isolated from chestnuts, were inoculated on four nuts. Chestnuts favored the production of the largest number of Penicillium toxic metabolites. The method was used for the analysis of 41 commercial samples: 71% showed to be contaminated by Penicillium-toxins. Cyclopenin and cyclopenol were the most frequently detected metabolites, with an incidence of 32% and 68%, respectively. Due to the risk of contamination of nuts with Penicillium-toxins, future studies and legislation should consider a larger number of mycotoxins.

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“…However, there are no legal limits for aflatoxins in all edible oils, although they have been set in oilseeds, i.e. 2.0 µg/kg for AFB1 and 4.0 µg/kg for the sum of the four aflatoxins (B1+B2+G1+G2) [39]. In view of their hazard to the health of the consumer, it is possible to eliminate them.…”

Section: Aflatoxins In the Oils Analyzedmentioning

confidence: 99%

Aflatoxins and Moisture Levels in Edible Oils Produced in Burkina Faso

Zio

Dembele

Bazié³

et al. 2020

EJNFS

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Aim: The study aim was to assess aflatoxin and moisture levels in edible oils produced and consumed in Burkina Faso to know the impact on consumer health. Methodology: A total of 61 samples of refined cottonseeds oils and crude peanut oils were collected from Ouagadougou, Bobo Dioulasso and surrounding areas. Aflatoxin B1 (AFB1), aflatoxin B2 (AFB2), aflatoxin G1 (AFG1) and aflatoxin G2 (AFG2) were determined by HPLC and moisture by differential weighing after oven drying. Results: The moisture content of peanut oils were ranged from 0.06 to 0.18% and cottonseeds oils from 0.02 to 0.17%. The moisture average is 0.13% for peanut oils and 0.08% for cottonseeds oils (P<0.05). The moisture of all oils is lower and conform to the Codex Alimentarius standard. AFB1, AFB2, AFG1 and AFG2 were identified in 86.89% of the oil samples analyzed. The proportion of samples contaminated with AFB1 is 57.38%, 59.02% for AFB2, 42.62% for AFG1 and 65.57% for AFG2. The AFB1 average of peanut oils is 6.21 ng/g while that of cottonseeds oils is 0.03 ng/g. The AFB2 average of peanut oils is 0.89 ng/g against 0.04 ng/g for cottonseeds oils (P<0.05). The AFG1 average of peanut oils is 0.54 ng/g and 0.08 ng/g for cottonseeds oils (P<0.05). The AFG2 average of peanut oils was 0.66 ng/g against 0.64 ng/g for cottonseeds oils. AFG2 had the highest proportion of all oils while AFB1 has the highest concentration and proportion in peanut oils. The 72.13% samples analyzed in this study comply with the European Community standard for aflatoxin B1 level maximum in oilseeds. Conclusion: Aside from the moisture content that comply with the standard, aflatoxins are present at varying levels and can negatively impact the consumer health. It is important to strengthen the monitoring and production system in order to have quality oil.

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A rapid method for the determination of mycotoxins in edible vegetable oils by ultra-high performance liquid chromatography-tandem mass spectrometry

Cited by 88 publications

References 22 publications

Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole Orbitrap High-Resolution Mass Spectrometry for Multi-Residue Analysis of Mycotoxins and Pesticides in Botanical Nutraceuticals

Ultra-High-Performance Liquid Chromatography Coupled with Quadrupole Orbitrap High-Resolution Mass Spectrometry for Multi-Residue Analysis of Mycotoxins and Pesticides in Botanical Nutraceuticals

HPLC-MS/MS Method for the Detection of Selected Toxic Metabolites Produced by Penicillium spp. in Nuts

Aflatoxins and Moisture Levels in Edible Oils Produced in Burkina Faso

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