A rapid method for the determination of piroxicam in plasma by high-performance liquid chromatography

Maya, Manuela T.; Pais, João P.; Morais, José

doi:10.1016/0731-7085(95)01282-p

Cited by 20 publications

(5 citation statements)

References 8 publications

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“…Meloxicam and piroxicam levels have been determined using a number of analytical methods [1][2][3][4][5][6][7][8][9][10][11][12][13][14][15][16][17][18][19]. Although mass spectrometry [1,2,10,14] can produce the highest detection sensitivity, it may not be readily available in all laboratories and is expensive.…”

Section: Introductionmentioning

confidence: 99%

“…In order to obtain a suitable limit of 2 Chromatography Research International quantification (LOQ) for the determination of meloxicam and piroxicam in biological samples several different processes have been used. Some of the methods employed include liquid-liquid extractions using diethyl ether [3,5,7,12,16] or methylene chloride [18,19], protein precipitation [4,6,13,17], precolumn enrichment [8,9,15], and solid phase extraction [11]. In many of the liquid-liquid extraction methods developed, large amounts of organic solvents are needed.…”

Section: Introductionmentioning

confidence: 99%

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High-Performance Liquid Chromatography Determination of Meloxicam and Piroxicam with Ultraviolet Detection

Cox

Hayes

Yarbrough

et al. 2014

Chromatography Research International

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A simple accurate and sensitive high-performance liquid chromatographic method for the determination of meloxicam and piroxicam concentrations in small volume plasma samples has been developed. Following a liquid extraction using chloroform, samples were separated by reversed-phase high-performance liquid chromatography on an XBridge C18 column (4.6 × 250 mm) and quantified using ultraviolet detection at 360 nm. The mobile phase was a mixture of water with glacial acetic acid (pH 3.0) and acetonitrile (50 : 50), with a flow rate of 1.0 mL/min. The standard curve ranged from 5 to 10,000 ng/mL for meloxicam in bearded dragon (Pogona vitticeps) plasma and piroxicam in crane (Grus rubicunda) plasma. Intra- and interassay variability for meloxicam and piroxicam were less than 10% and the average recovery was greater than 90% for both drugs. This method was developed in bearded dragon and crane plasma and should be applicable to any species, making it useful for those investigators dealing with small sample volumes, particularly when conducting pharmacokinetics studies which require multiple sampling from the same animal.

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Section: Introductionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

High-Performance Liquid Chromatography Determination of Meloxicam and Piroxicam with Ultraviolet Detection

Cox

Hayes

Yarbrough

et al. 2014

Chromatography Research International

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“…Several analytical methods have been described for the determination of Piroxicam, including thin-layer chromatography (TLC) [3][4][5][6], capillary electrophoresis (CE) [7], spectrofluorometry [8], derivative spectrometry [9][10][11] and high performance liquid chromatography (HPLC) [12][13][14][15].…”

Section: Introductionmentioning

confidence: 99%

Quantitative determination of Piroxicam by TLC–MALDI TOF MS

Crecelius

Clench

Richards

et al. 2004

Journal of Pharmaceutical and Biomedical Analysis

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“…Since then, several chromatographic methods have been reported for the determination of PX in biological fluids. [6][7][8][9][10][11][12][13][14] However, most of these methods, while providing a high degree of sensitivity, are complicated and time consuming, which led us to search for a simple, rapid, reliable and specific method for the determination of PX and its major metabolite OHPX in human plasma. The classical UV/VIS spectrophotometric method presents the severe problem of overlapping spectral bands of PX, OHPX and the plasma background, which makes the simultaneous determination of these drugs impossible in this matrix.…”

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confidence: 99%

Simultaneous determination of piroxicam and its major metabolite 5′-hydroxypiroxicam in human plasma by derivative spectrophotometry

Ródenas¹

1998

Analyst

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A first-derivative spectrophotometric method for the simultaneous determination of piroxicam (PX) and its major metabolite 5'-hydroxypiroxicam (OH PX) in human plasma is described. The method consists of direct extraction of the two drugs from the plasma samples with hydrochloric and trichloroacetic acid prior to their determination by measuring the first-derivative signals at 337.0 nm for PX and at 327.0 nm for OHPX (zero-crossing wavelength). The calibration graphs were linear up to 10.0 and 8.0 mg l-1 of PX and OH PX, respectively, and the limits of quantification were 0.27 and 0.56 mg l-1. The possible interfering effects of other substances were studied.

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A rapid method for the determination of piroxicam in plasma by high-performance liquid chromatography

Cited by 20 publications

References 8 publications

High-Performance Liquid Chromatography Determination of Meloxicam and Piroxicam with Ultraviolet Detection

High-Performance Liquid Chromatography Determination of Meloxicam and Piroxicam with Ultraviolet Detection

Quantitative determination of Piroxicam by TLC–MALDI TOF MS

Simultaneous determination of piroxicam and its major metabolite 5′-hydroxypiroxicam in human plasma by derivative spectrophotometry

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