A rapid method of evaluating permeabilizing activity against Pseudomonas aeruginosa

Ayres, H. M.; Furr, J. R.; Russell, A. D.

doi:10.1111/j.1472-765x.1993.tb00381.x

Cited by 29 publications

(26 citation statements)

References 6 publications

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“…2000) have also been evaluated with conductance technique in broths and milk, respectively. Due to these reasons, the impedimetric method is recognized as an alternative rapid method not only for screening the biocide activity of novel antimicrobial agents, but also for estimation of growth kinetics in mathematical modeling (Ayres et al. 1993, 1998; Johansen et al.…”

Section: Introductionmentioning

confidence: 99%

A newly developed assay to study the minimum inhibitory concentration of Satureja spinosa essential oil

et al. 2006

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Aims: The minimum inhibitory concentration (MIC) of Satureja spinosa essential oil against Staphylococcus aureus, Escherichia coli O157:H7, Listeria monocytogenes, Salmonella enterica, Salmonella serovar Enteritidis PT4 and Bacillus cereus was comparatively assessed with an established optical density method as well as a novel impedimetric method. Methods and Results: The impedimetric analysis takes into account information of microbial growth, such as detection time, maximum conductance, and slope of the conductance curve. For each pathogen two levels of inoculation were studied, a high (105 CFU ml−1) and a low level (102 CFU ml−1). Non‐linear regression analysis was used to fit the data using a modification of a previously published model, from which a more exact value can be obtained for the MIC. Both methods gave similar MICs as shown by t‐test statistical analysis. Salm. Enteritidis seems to be the least sensitive to the action of S. spinosa essential oil followed by L. monocytogenes, E. coli, B.cereus and Staph. aureus. The MICs of low inoculum were lower than that of high inoculum. Conclusions: The new impedimetric assay of MIC of essential oils can be considered a reliable rapid method for screening antimicrobial effectiveness of natural additives. Significance and Impact of the Study: Determination of the minimum inhibitory concentration of an essential oil with the simple conductance technique and further study of the mode of action of its components is a good combination for obtaining additional knowledge for industrial application of such natural additives.

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Section: Introductionmentioning

confidence: 99%

A newly developed assay to study the minimum inhibitory concentration of Satureja spinosa essential oil

et al. 2006

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“…Previous work (Ayres et al 1993) has demonstrated that EDTA and other potential permeabilizing agents induced lysozyme sensitivity in a strain of Ps. aeruginosa.…”

Section: Introductionmentioning

confidence: 99%

“…The outer membrane (OM) of Ps. aeruginosa requires a high concentration of Mg 2¦ cations for stabilization (Brown 1975) and cells are prone to lysis by chelating agents (Ayres et al 1993). External Mg 2¦ antagonizes EDTA or competes with OM Mg 2¦ for the chelator (Vaara and Jaakkola 1989;Vaara 1992).…”

Section: Introductionmentioning

confidence: 99%

Effect of divalent cations on permeabilizer-induced lysozyme lysis of Pseudomonas aeruginosa

Ayres

Furr

Russell

1998

Lett Appl Microbiol

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US SE L L. 1998. The presence of divalent (Mg 2+ ) ions greatly reduced the lysis of Pseudomonas aeruginosa strain G48 in a system at pH 7·8 or 9·0 consisting of ethylenediamine tetraacetic acid (EDTA), lysozyme and tris. Similar reductions in lysis occurred when EDTA was replaced by nitrilotriacetic acid, sodium citrate or sodium polyphosphate. The effect depended on the cation concentration. Mg 2+ may replace cations removed from the outer membrane, or may effectively remove the permeabilizer from the system. The results suggest that the permeabilizing activity associated with these agents against this organism has a common basis in affecting the outer membrane.

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“…During a 0.25 mg/L ozonated water treatment, the number of vesicles on cell surfaces also increased with an increase in contact time. Cell membrane permeation increased and extracellular constituents were more prevalent during the process, in Agent-induced lysis of P. aeruginosa cells exposed to ozone Cells treated with EDTA or other chelating agents are known to become sensitive to antibacterial agents to which they are usually resistant (13). Ironically, antibacterial agents also influence bacterial cell lysis induced by EDTA or other agents (14)(15)(16).…”

Section: Resultsmentioning

confidence: 97%

Effects of ozone on the cytomembrane and ultrastructure of Pseudomonas aeruginosa

Zhang

et al. 2015

Food Sci Biotechnol

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The ozone impact on Pseudomonas aeruginosa ATCC27853 cells was studied. Ozone-induced loss of K + , Mg 2+ , and ATP was measured using inductively coupled plasma/mass spectrometry and a bioluminescence assay. Maximum releases of K + and Mg 2+ were achieved at 0.37 mg/L of ozone after 2 min with a killing rate of culturable bacteria greater than 93%. Maximum release of ATP was attained at 0.42 mg/L of ozone after 3 min. Transmission electron microscopy showed that shapes of treated cells were integrated, but cytoplasmic agglutinations and vacuoles appeared. Ozone treatment caused lysis of P. aeruginosa cells to be sensitive to sodium dodecyl sulfate, insensitive to NaOH, and subject to inactivation by proteinase K. A combined action of cytoplasm agglutination, protein denaturation, and membrane permeabilization, rather than cell lysis, leads to non-culturability of P. aeruginosa cells.

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A rapid method of evaluating permeabilizing activity against Pseudomonas aeruginosa

Cited by 29 publications

References 6 publications

A newly developed assay to study the minimum inhibitory concentration of Satureja spinosa essential oil

A newly developed assay to study the minimum inhibitory concentration of Satureja spinosa essential oil

Effect of divalent cations on permeabilizer-induced lysozyme lysis of Pseudomonas aeruginosa

Effects of ozone on the cytomembrane and ultrastructure of Pseudomonas aeruginosa

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