A Rapid Method of Fluorescent in situ Hybridization (FISH) to Paraffin-Embedded Tissues for Sexing Human Embryos and Fetuses: Optimal Conditions of Fixation and Proteinase K Treatment.

Nakamura, Noriko; Mori, Chisato; Narita, Megumi; Uwabe, Chigako; Shiota, Kohei

doi:10.1267/ahc.27.81

Cited by 2 publications

(1 citation statement)

References 16 publications

(13 reference statements)

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“…Karyotyping of such specimens is often hampered by low mitotic indices, and the necessity to culture cells may veil the visualization of crucial aberrations due to selective growth of certain cell populations. Recently, several studies were published performing FISH on sections of paraffin embedded embryonic material with DNA probes specific for repetitive centromeric sequences to retrospectively determine the embryos genomic constitution (Mori and Shiota,1994;Nakamura et al, 1994;Van Lijnschoten et al, 1994). If sufficient DNA can be extracted from archival abortion material, and subsequently be amplified by DOP-PCR, retrospective CGH analyses become feasible.…”

Section: Comparative Genom/c Hybrid/saf/on Fcghjmentioning

confidence: 99%

Genomic constitution of gametes and preimplantation embryos

Coonen¹

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Section: Comparative Genom/c Hybrid/saf/on Fcghjmentioning

confidence: 99%

Genomic constitution of gametes and preimplantation embryos

Coonen¹

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Application of in-situ hybridization techniques to study human preimplantation embryos: a review

Coonen¹

1998

Human Reproduction Update

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Although the first in-situ hybridization (ISH) experiments were performed more than two decades ago, it was not until 1987 that the technique was implemented in the study of preimplantation. Since then, several groups have explored the feasibility of using the ISH technique as a diagnostic tool to study the genomic constitution of human preimplantation embryos. Though a fair number of such ISH studies have been published and unravelling of the embryos' genomic secrets proceeds with great strides, there are still many challenges in this area of research. The scope of this review is to outline the research and diagnostic applications of single and multicolour (fluorescence) ISH to study the chromosomal content of human preimplantation embryos and to discuss the implications for human preimplantation genetic diagnosis. Current hybridization techniques are being discussed, including the capabilities and pitfalls of this approach when applied to human preimplantation embryos. Finally, future perspectives for the ISH technique in studying the genomic constitution of preimplantation embryos are reviewed.

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A Rapid Method of Fluorescent in situ Hybridization (FISH) to Paraffin-Embedded Tissues for Sexing Human Embryos and Fetuses: Optimal Conditions of Fixation and Proteinase K Treatment.

Cited by 2 publications

References 16 publications

Genomic constitution of gametes and preimplantation embryos

Genomic constitution of gametes and preimplantation embryos

Application of in-situ hybridization techniques to study human preimplantation embryos: a review

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