A rapid method to deplete endogenous DNA-binding proteins from reticulocyte lysate translation systems

Ebel, Thorsten T.; Sippel, Albrecht E.

doi:10.1093/nar/23.11.2076

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Trna-mediated /Lroteill {Tngilleering (Trampe)1

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1998

2012

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Cited by 4 publications

(2 citation statements)

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“…In certain scenarios, wheal gernl-based extracts are preferred since they lack endogenous 1l1anlnlalian transcription factors. Recently., a rnethod has been reported that renl0ves endogenous DNA-binding proteins froll1 the reticulocyte systenl prior to the translation reaction (Ebel and Sippel, 1995).…”

Section: Trna-mediated /Lroteill {Tngilleering (Trampe)mentioning

confidence: 99%

Cell-Free Protein Synthesis Systems: Biotechnological Applications

Gite¹,

Lim²,

Rothschild

2006

Biotechnology and Genetic Engineering Reviews

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Section: Trna-mediated /Lroteill {Tngilleering (Trampe)mentioning

confidence: 99%

Cell-Free Protein Synthesis Systems: Biotechnological Applications

Gite¹,

Lim²,

Rothschild

2006

Biotechnology and Genetic Engineering Reviews

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“…Many researchers investigating transcription‐factor binding use coupled wheat germ rather than rabbit reticulocyte translation systems, as wheat germ extract does not contain endogenous transcription factors such as NFκB (Sif and Gilmore, ). If the reticulocyte system must be used, it is possible to remove endogenous DNA‐binding proteins from the reticulocyte system by a rapid method using biotinylated DNA and streptavidin‐conjugated magnetic beads (Ebel and Sippel, ).…”

Section: New Applicationsmentioning

confidence: 99%

Overview of Eukaryotic In Vitro Translation and Expression Systems

Jagus¹,

Beckler

1998

CP Cell Biology

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The ability to investigate cellular processes in vitro permits detailed analysis of the process and its molecular components. Eukaryotic translation and expression is one system that has been well studied. This overview describes the development of in vitro systems, including such approaches as continuous‐flow systems, coupled transcription/translation, and the incorporation of non‐natural amino acids. It also discusses molecular and genetic studies to probe translation, including post‐translational fate of the synthesized proteins.

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