A rapid method with ultra‐high‐performance liquid chromatography–tandem mass spectrometry for simultaneous determination of five type B trichothecenes in traditional Chinese medicines

Abstract

A speedy and selective ultra-HPLC-MS/MS method for simultaneous determination of deoxynivalenol (DON), 3-acetyldeoxynivalenol (3-ADON), 15-ADON, nivalenol and fusarenon X in traditional Chinese medicines (TCMs) was developed. The method was based on one-step sample cleanup using reliable homemade cleanup cartridges. A linear gradient mobile-phase system, consisting of water containing 0.2% aqueous ammonia and acetonitrile/methanol (90:10, v/v) at a flow rate of 0.4 mL/min, and an Acquity UPLC HSS T3 column (100 mm x 2.1 mm, 1.8 microm) were employed to obtain the best resolution of the target analytes. [(13)C(15)]-DON was used as the internal standard to accomplish as accurate as possible quantitation. The established method was further validated by determining the linearity (R(2) > or = 0.9990), sensitivity (LOQ, 0.29-0.99 microg/kg), recovery (88.5-119.5%) and precision (RSD < or = 15.8%). It was shown to be a suitable method for simultaneous determination of DON, 3-ADON, 15-ADON, nivalenol and fusarenon X in various TCM matrices. The utility and practical impact of the method was demonstrated using different TCM samples.

Cumulative health risk assessment of co-occurring mycotoxins of deoxynivalenol and its acetyl derivatives in wheat and maize: Case study, Shanghai, China

“…In principle, when the point estimate is used for risk assessment, the variability and uncertainty of the food consumption and the contamination levels are not considered, resulting in the overestimation of the real exposure (Haighton et al, 2012;Han et al, 2010). To account for the abovementioned shortcomings, a full probabilistic model (Monte Carlo simulation) is recommended (Nakatani et al, 2011) as was the case in this study.…”

A method of analysis for T-2 toxin and neosolaniol by UPLC-MS/MS in apple fruit inoculated withTrichothecium roseum

“…Several methods have been reported for the analysis of trichothecenes (Lanoseth & Elen 1996;Avantaggiato et al 2004;Han et al 2010). However, most of these methods were developed for cereal grain such as wheat, barley, oat, maize, rice and their derived products (i.e.…”

Predominant mycobiota and aflatoxin content in Brazil nuts

“…According to Häubl et al (2005), to circumvent the matrix effects, internal (stable) standards can be used. An internal standard is an ideal analyte molecule labelled with an isotope, usually prepared by organic synthesis to substitute some 12 C atoms with 13 C atoms (Han et al 2010). Table 3 also shows that the current HPLC-FD method for AF has LOD and LOQ that are lower than These results are favourable when compared with those from previous years.…”