A rapid micro-agglutination technique for the detection of antibody to Legionella pneumophila serogroup 5

Constantine, C. E.; Wreghitt, T.G.

doi:10.1099/00222615-34-1-29

Cited by 6 publications

(4 citation statements)

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“…On readmission, he had fever (38.5°C), pulse of 160/min, respiration 24/min, and altered mental status. His white blood cell count (WBC) was 27,600/mm 3 , with 92% polymorphonuclear leukocytes. Chest radiographs showed bilateral, multiple, newly developed rounded opacities.…”

Section: Resultsmentioning

confidence: 99%

“…Four of 5 patients with L. pneumophila serogroup 5 disease previously died [2][3][4][5]: 2 sisters with underlying systemic lupus erythematosus treated with prednisone developed pulmonary nodules and microabscesses; I patient with carcinoma of esophagus developed pleural empyema, and 1 had chronic lymphocytic leukemia.…”

Section: Discussionmentioning

confidence: 99%

“…In contrast, only 6 patients with legionnaires' disease caused by L. pneumophila serogroup 5 were reported to the Centers for Disease Control and Prevention between 1980 and 1989 [1]. In a review of the literature, we found only 5 reported patients with L. pneumophila serogroup 5 disease [2][3][4][5]. Accordingly, little is known about the epidemiology and clinical manifestations of infection caused by L. pneumophila serogroup 5.…”

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confidence: 89%

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Nosocomial Legionnaires' Disease Caused by Legionella pneumophila Serogroup 5: Laboratory and Epidemiologic Implications

Chang

Jacobs

Colodny

et al. 1996

Journal of Infectious Diseases

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Environmental monitoring and clinical surveillance for Legionella species were done for 12 months as recommended by the Allegheny County Health Department (Pittsburgh). The water system of a hospital was found to be colonized with Legionella pneumophila serogroup 5. Three patients with nosocomial L. pneumophila serogroup 5 disease were subsequently diagnosed after laboratory tests for legionellae were made available for all patients with nosocomial pneumonia. All serogroup 5 isolates from the hospital water matched the 3 patient isolates by pulsed-field gel electrophoresis (PFGE). Furthermore, isolates found in the water supply dating back 10 years showed the same PFGE pattern. In contrast, 12 L. pneumophila serogroup 5 isolates from eight other institutions had different PFGE patterns. Routine environmental cultures were important in stimulating the application of Legionella laboratory testing, which subsequently identified unsuspected patients with nosocomial legionnaires' disease.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 89%

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Nosocomial Legionnaires' Disease Caused by Legionella pneumophila Serogroup 5: Laboratory and Epidemiologic Implications

Chang

Jacobs

Colodny

et al. 1996

Journal of Infectious Diseases

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“…Colon tissues were fixed in 4% formalin, and immunohistochemical and immunofluorescence staining was performed for human and mouse colon sections at the Histomorphology Platform of Zhejiang University, following the manufacturer's standard protocol (reagents purchased from Beijing Zhongshan Jinqiao Biotechnology Company). Human colon specimens were scored using Constantine's protocol (65), under high magnification, and integrated staining intensity and the percentage of positive cells were scored.…”

Section: Author Contributionsmentioning

confidence: 99%

Fbxw7 increases CCL2/7 in CX3CR1hi macrophages to promote intestinal inflammation

Song

et al. 2019

Journal of Clinical Investigation

103

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2E) and F4/80 + macrophages (Figure 2F) after DSS treatment for 5 days compared with expression in the colons of healthy mice. These results suggest that increased FBXW7 expression in monocytes and macrophages was correlated with local colonic inflammation in both humans and mice. Fbxw7 deficiency attenuates experimental colitis. To investigate the role of Fbxw7 in macrophages in colitis, LysM-Cre + Fbxw7 fl/fl (LysM + Fbxw7 fl/fl) mice and their control littermates (Fbxw7 fl/fl) were subjected to acute colitis induction using 3% DSS. Colitisinduced macroscopic changes (body weight loss, diarrhea, and rectal bleeding) were significantly alleviated in the LysM + Fbxw7 fl/fl mice compared with Fbxw7 fl/fl littermates (Figure 3A). LysM + Fbxw7 fl/fl mice sacrificed on day 9 displayed significantly longer colons (Figure 3B), milder epithelial damage, and deceased areas of mucosal ulceration (Figure 3C and Supplemental Figure 2A) compared with Fbxw7 fl/fl littermates. Moreover, expression levels of the tight junction genes Cldn1, Cldn2, Ocln, and Tjp1 (Supplemental Figure 2B) and of TJP1 protein (Supplemental Figure 2C) were significantly higher in the epithelia of LysM + Fbxw7 fl/fl mice compared with Fbxw7 fl/fl littermates after DSS treatment, which indicated that the epithelial barrier integrity was less disrupted in mice with myeloid-specific Fbxw7 deficiency. At the same time, LysM + Fbxw7 fl/fl mice showed significantly improved survival rates compared with Fbxw7 fl/fl littermates after 4% DSS treatment (Figure 3D), indicating that Fbxw7 deficiency protects mice from DSS-induced colitis. During the recovery period of intestinal inflammation, the rate of body weight gain was more rapid in LysM + Fbxw7 fl/fl mice than in Fbxw7 fl/fl littermates (Figure 3E). Moreover, LysM + Fbxw7 fl/fl mice had longer colon lengths than did their Fbxw7 fl/fl littermates (Figure 3F) on day 15. Similarly, TNBS-induced colon shortening, the disease activity index (DAI), body weight loss, and epithelial damage were also alleviated in LysM + Fbxw7 fl/fl mice compared with Fbxw7 fl/fl littermates (Supplemental Figure 3, AD). These findings indicate that myeloid Fbxw7 deficiency attenuates experimental colitis. Fbxw7 deficiency decreases proinflammatory MPh accumulation. Microbiota-induced inflammation is critical for the regulation of intestinal homeostasis. To determine whether the decreased DSS colitis susceptibility in LysM + Fbxw7 fl/fl mice was mediated by shifts in gut microbiota, we analyzed the fecal microbiota composition in LysM + Fbxw7 fl/fl mice and Fbxw7 fl/fl littermates. The relative abundance of bacteria at the phylum level and the species composition cluster according to operational taxonomic units (OTUs) among LysM + Fbxw7 fl/fl mice and Fbxw7 fl/fl littermates are shown in Supplemental Figure 4A and Figure 4A, respectively. Moreover, microbial community diversity (Figure 4B) and microbial community composition (Supplemental Figure 4B) were not significantly different between LysM + Fbxw7 fl/fl mice and Fbxw7 fl/fl li...

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Detection and occurrence of waterborne bacterial and viral pathogens

Emde¹,

Mao²,

Finch³

1992

Water Environment Research

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A rapid micro-agglutination technique for the detection of antibody to Legionella pneumophila serogroup 5

Cited by 6 publications

References 3 publications

Nosocomial Legionnaires' Disease Caused by Legionella pneumophila Serogroup 5: Laboratory and Epidemiologic Implications

Nosocomial Legionnaires' Disease Caused by Legionella pneumophila Serogroup 5: Laboratory and Epidemiologic Implications

Fbxw7 increases CCL2/7 in CX3CR1hi macrophages to promote intestinal inflammation

Detection and occurrence of waterborne bacterial and viral pathogens

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