A rapid mismatch polymerase chain reaction assay to detect carriers of complex vertebral malformation in Holstein cattle

Wang, Shuai; Huang, Hao; Zhao, Xiao‐Fan; Zhu, Huabin; Du, Weihua; Wang, Dong; Liu, Yan; Tong, Qin; Wang, Zongli

doi:10.1177/1040638712441605

Cited by 5 publications

(3 citation statements)

References 22 publications

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“…Twenty-six and thirty-four CVM carriers were identified, corresponding to the heterozygote carrier frequency of 21.6% (26) and 34% ( 14) respectively in Iran, and no mutant homozygote was found. In China, it has been reported that the frequency of the carrier animals are 10.48% (33), 2.92 % (29) and 15.58% (30) in Holstein cattle. In our country, Kulaklı and Akyüz (17) did not find carriers of CVM.…”

Section: Holştayn ıRkı Sığırlarda Sığır Lökosit Bağlanma Yetmezliği Faktör XI Eksikliği Ve Kompleks Vertebral Malformasyon Hastalıkları Gmentioning

confidence: 99%

Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle

Cakmak¹,

Yardibi²

2019

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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Hereditary diseases cause yield and economic loses. It is important to examine hereditary diseases at the molecular level and to remove diseases from the herd. In our study, it was aimed to determine allele frequencies of genes that cause bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation diseases in Holstein cattle. Blood samples were randomly taken from 300 Holstein cattle in different dairy farms in Kocaeli, Sakarya and Balıkesir provinces. Deoxyribonucleic acid samples were isolated from blood samples by using the standard ammonium acetate salt-out method. The target regions were amplified by polymerase chain reaction to determine the mutant alleles causing bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation. According to the nucleotide chromotograms of the samples subjected to bovine leukocyte adhesion deficiency analysis, it was determined that 4 out of 300 cattle were heterozygous and 296 were homozygous. Polymerase chain reaction procedure for factor XI deficiency disease was sufficient, while samples amplified by polymerase chain reaction for complex vertebral malformation disease were subjected to restriction particle length polymorphism. Factor XI deficiency and complex vertebral malformation disease genes were all homozygous normal.

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Section: Holştayn ıRkı Sığırlarda Sığır Lökosit Bağlanma Yetmezliği Faktör XI Eksikliği Ve Kompleks Vertebral Malformasyon Hastalıkları Gmentioning

confidence: 99%

Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle

Cakmak¹,

Yardibi²

2019

Ankara Üniversitesi Veteriner Fakültesi Dergisi

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“…The prevalence of CVM carriers in Thrace region of Turkey (3.2%) was lower than those for Holstein cattle reared in Japan (32.5% and 13.0%) [ 11 16 ], Denmark (31.0%) [ 24 ], Poland (24.8%) [ 20 ], Sweden (23.0%) [ 5 ], Germany (13.2%) [ 14 ], China (15.6%, 14.7%, 9.54%, and 7.7%) [ 7 23 26 27 ], and Slovakia (8.5%) [ 10 ], but it was higher than that in Iran [ 12 19 ] (0%). The instructions on procedures and principles associated with sperm imports to Turkey was revised for CVM-carrier bull sperm by the Ministry of Food Agriculture and Livestock in 2007.…”

Section: Discussionmentioning

confidence: 99%

“…Thereafter, sporadic CVM cases were reported from the USA [ 9 ] and the United Kingdom [ 18 ]. Subsequently, studies to identify CVM carriers were conducted in Japan [ 11 16 ], Germany [ 14 ], Sweden [ 5 ], Czech Republic [ 8 ], Poland [ 20 ], Turkey [ 15 ], China [ 7 23 26 27 ], Slovakia [ 10 ], and Iran [ 12 19 ]. Allele-specific polymerase chain reaction (AS-PCR) [ 11 ], created restriction-site PCR (CRS-PCR) [ 25 ], PCR with primer-introduced restriction analysis (PCR-PIRA) [ 13 ], and DNA sequencing [ 15 ] methods have been reported as capable of detecting the mutant allele that causes the CVM disorder.…”

Section: Introductionmentioning

confidence: 99%

Comparison of allele-specific PCR, created restriction-site PCR, and PCR with primer-introduced restriction analysis methods used for screening complex vertebral malformation carriers in Holstein cattle

Avanus

Altınel

2017

J Vet Sci

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Complex vertebral malformation (CVM) is an inherited, autosomal recessive disorder of Holstein cattle. The aim of this study was to compare sensitivity, specificity, positive and negative predictive values, accuracy, and rapidity of allele-specific polymerase chain reaction (AS-PCR), created restriction-site PCR (CRS-PCR), and PCR with primer-introduced restriction analysis (PCR-PIRA), three methods used in identification of CVM carriers in a Holstein cattle population. In order to screen for the G>T mutation in the solute carrier family 35 member A3 (SLC35A3) gene, DNA sequencing as the gold standard method was used. The prevalence of carriers and the mutant allele frequency were 3.2% and 0.016, respectively, among Holstein cattle in the Thrace region of Turkey. Among the three methods, the fastest but least accurate was AS-PCR. Although the rapidity of CRS-PCR and PCR-PIRA were nearly equal, the accuracy of PCR-PIRA was higher than that of CRS-PCR. Therefore, among the three methods, PCR-PIRA appears to be the most efficacious for screening of mutant alleles when identifying CVM carriers in a Holstein cattle population.

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Congenital, familial, and genetic disorders

2022

Large Animal Neurology

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A rapid mismatch polymerase chain reaction assay to detect carriers of complex vertebral malformation in Holstein cattle

Cited by 5 publications

References 22 publications

Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle

Detection of allele and genotype frequencies of bovine leukocyte adhesion deficiency, factor XI deficiency and complex vertebral malformation disease genes in Holstein cattle

Comparison of allele-specific PCR, created restriction-site PCR, and PCR with primer-introduced restriction analysis methods used for screening complex vertebral malformation carriers in Holstein cattle

Congenital, familial, and genetic disorders

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